Phagocytosis of mycobacteria by zebrafish macrophages is dependent on the scavenger receptor Marco, a key control factor of pro-inflammatory signalling

Benard, Erica L.; Roobol, Stefan J.; Spaink, Herman P.; Meijer, Annemarie H.

doi:10.1016/j.dci.2014.07.022

Cited by 46 publications

(33 citation statements)

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“…In human alveolar macrophages, a specific protein that bound inhaled particles and bacteria was established to be MARCO [10]. Later, people used Mycobacterium marinum , a close relative of Mtb, to infect zebrafish, and found MARCO knockdown zebrafish suffered an increased bacterial burden [11]. Another study described how mice without MARCO showed a delay in the organization of marginal zone macrophages within the spleen [12].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the relationship between MARCO and CD36 single-nucleotide polymorphisms and susceptibility to pulmonary tuberculosis in a Chinese Han population

et al. 2017

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BackgroundGene polymorphisms impact greatly on a person’s susceptibility to pulmonary tuberculosis (PTB). Macrophage receptor with collagenous structure (MARCO) and CD36 are two scavenger receptors (SRs) that can recognize Mycobacterium tuberculosis (Mtb) and play a key role in tuberculosis infection. Gene polymorphisms of MARCO and CD36 may contribute to tuberculosis risk.MethodsTo investigate whether genetic polymorphisms of MARCO and CD36 are associated with susceptibility to PTB, genomic DNA samples from patients (n = 202) and healthy controls (n = 216) were collected and analyzed by polymerase chain reaction with high-resolution melting analysis.ResultsWe studied two single nucleotide polymorphisms (SNPs) in MARCO (rs12998782 and rs17009726) and three SNPs in CD36 (rs1194182, rs3211956 and rs10499859). Rs12998782 (P = 0.018) might be associated with susceptibility to PTB. Rs1194182 (P < 0.01) and rs10499859 (P < 0.001) might be associated with resistance to PTB. Rs17009726 and rs3211956 were not associated with susceptibility/resistance to PTB.ConclusionsThese data showed that MARCO rs12998782 may increase PTB risk while two SNPs of CD36, rs1194182 and rs10499859 may reduce the risk, indicating MARCO and CD36 as important receptors in response to PTB.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-017-2595-2) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the relationship between MARCO and CD36 single-nucleotide polymorphisms and susceptibility to pulmonary tuberculosis in a Chinese Han population

et al. 2017

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“…Marco is a class A scavenger receptor that serves as a pattern-recognition receptor for bacteria and is involved in host defense. [38][39][40] Intriguingly, when the functions of the remaining genes were annotated, 3 additional genes with putative roles in the recognition and removal of microbial pathogens were identified: Colec12, cdc42bpa, and Wfdc10. Colec12 encodes for scavenger receptor C-type lectin and has been implicated in recognition and phagocytosis of fungi and bacteria, 41,42 whereas cdc42 is required for cytoskeletal rearrangement during phagocytosis.…”

Section: Gene Expression Differences Between Host-and Donor-derived Amsmentioning

confidence: 99%

Transcriptome analysis highlights the conserved difference between embryonic and postnatal-derived alveolar macrophages

Gibbings

Goyal

Desch³

et al. 2015

Blood

195

188

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• Of the 30 000 genes, there are ;0.1% genes whose expression is linked to the origin of the cell rather than the environment.• Marco was most conserved by embryonic origin and not altered by the environment, whereas C1qb and Plbd1 were most conserved by adult origin.Alveolar macrophages (AMs) reside on the luminal surfaces of the airways and alveoli where they maintain host defense and promote alveolar homeostasis by ingesting inhaled particulates and regulating inflammatory responses. Recent studies have demonstrated that AMs populate the lungs during embryogenesis and self-renew throughout life with minimal replacement by circulating monocytes, except under extreme conditions of depletion or radiation injury. Here we demonstrate that on a global scale, environment appears to dictate AM development and function. Indeed, transcriptome analysis of embryonic host-derived and postnatal donor-derived AMs coexisting within the same mouse demonstrated >98% correlation and overall functional analyses were similar. However, we also identified several genes whose expression was dictated by origin rather than environment. The most differentially expressed gene not altered by environment was Marco, a gene recently demonstrated to have enhancer activity in embryonic-derived but not postnatal-derived tissue macrophages. Overall, we show that under homeostatic conditions, the environment largely dictates the programming and function of AMs, whereas the expression of a small number of genes remains linked to the origin of the cell. (Blood. 2015;126(11):1357-1366 Introduction Alveolar macrophages (AMs) reside on the luminal surfaces of the airways and airspaces where they serve critical roles in host defense and alveolar homeostasis, ingesting particulates and microbes that are constantly encountered in the lungs. Importantly, under most circumstances the phagocytosis of inhaled foreign agents is silent, such that inflammatory responses are activated only under circumstances when host defenses become overwhelmed.1 Indeed, compared with macrophages from other sites, AMs are relatively ineffective at initiating immune responses.2,3 Furthermore, compared with other tissue macrophages, they display a unique repertoire of cell surface molecules and have a distinct transcriptome profile. [4][5][6] AMs are now known to derive primarily from fetal liver monocytes and self-renew throughout life with minimal replenishment from circulating monocytes. [7][8][9][10][11][12][13][14][15] This self-renewal is not only maintained under steady-state conditions, but also during acute and chronic inflammation.16 These concepts were illustrated in lung-protected bone marrow (BM) chimera studies in which lead shields were used to protect AMs during radiation. Eight weeks after BM transplantation, the lungs of these chimeras contained AMs of host origin, whereas circulating monocytes were donor-derived. 16 In these chimeric mice, we showed that during inflammation (lipopolysaccharide or influenza A infection), BM donor-derived monocytes were rapidly...

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“…Particularly for the top ten blotch DE transcripts, six showed similar expression patterns in egg-spots, corresponding to two duplicated iridophore-related genes ( pnp4a and pnp5a) , the immunity-related gene ly6d , the ligand transporter-related gene ( ApoD) , and the two genes associated with egg-spots formation ( fhl2a and fhl2b) (Santos et al 2014) (Table 1). Besides, two transcripts were highly expressed in egg-spots, but low expressed in the blotch, corresponding to a novel transcript ENSONIT00000023612 and the immunity-related gene steap4 (Benard et al 2014) (Table 2). Fifteen transcripts were over-expressed in the blotch but down-regulated in egg-spots (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Revelation of the Genetic Basis for Convergent Innovative Anal Fin Pigmentation Patterns in Cichlid Fishes

Zhang

Xia

2017

Preprint

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Determining whether convergent novelties share a common genetic basis is vital to understanding the extent to which evolution is predictable. The convergent evolution of innovative anal fin pigmentation patterns in cichlid fishes is an ideal model for studying this question. Here, we focused on two patterns: 1) egg-spots, circular pigmentation patterns with different numbers, sizes and positions; and 2) the blotch, irregular pattern with no variation among species. How these two novelties originate and evolve remains unclear. Based on a thorough comparative transcriptomic and genomic analysis, we observed a common genetic basis with high evolutionary rates and similar expression levels between egg-spots and the blotch. But the associations of common genes with transcription factors and signalling pathways in the core gene network, as well as the integration of advantageous genes were observed specifically for egg-spots. Focusing on the whole transcriptomic level instead of limited numbers of candidate genes can explain the opposite conclusion between our study and the one from Santos et al. (2016), which suggested that no common genetic basis is shared between the blotch and egg-spots. Here, we propose that the re-use of the common genetic basis indicates important conservative functions (e.g., toolkit genes) for the origin of these convergent novel phenotypes, whereas independently evolved associations of common genes with transcription factors and signalling pathways (intrinsic factor) can free the evolution of egg-spots, and together with the integration of advantageous genes (extrinsic factor) can provide a clue to link egg-spots as a key innovation to the adaptive radiation in cichlid fishes. This hypothesis will further illuminate the mechanism of the origin and evolution of novelties in a broad sense.

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Phagocytosis of mycobacteria by zebrafish macrophages is dependent on the scavenger receptor Marco, a key control factor of pro-inflammatory signalling

Cited by 46 publications

References 69 publications

Evaluation of the relationship between MARCO and CD36 single-nucleotide polymorphisms and susceptibility to pulmonary tuberculosis in a Chinese Han population

Evaluation of the relationship between MARCO and CD36 single-nucleotide polymorphisms and susceptibility to pulmonary tuberculosis in a Chinese Han population

Transcriptome analysis highlights the conserved difference between embryonic and postnatal-derived alveolar macrophages

Revelation of the Genetic Basis for Convergent Innovative Anal Fin Pigmentation Patterns in Cichlid Fishes

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