Inhibition of Epithelial to Mesenchymal Transition by E-cadherin Up-regulation via Repression of Slug Transcription and Inhibition of E-cadherin Degradation

Abstract

Background: Epithelial-mesenchymal transition (EMT) is an important program in tumor metastasis. Results: SMAR1 inhibits EMT by up-regulating E-cadherin in a dual manner via repression of Slug transcription and inhibition of E-cadherin degradation. Conclusion: SMAR1 functions as a critical protein in regulating EMT. Significance: This study provides a potential mechanism for the contribution of SMAR1 in inhibiting breast cancer metastasis.

A new role for the PI3K/Akt signaling pathway in the epithelial-mesenchymal transition

“…Slug is also an important molecule in the EMT in cancer cells. 41 Slug overexpression in breast cancer can simultaneously downregulate E-cadherin, CK8, and CK9. When Slug is silenced in prostate cells, cellular proliferation is inhibited, the cell cycle is arrested at the G0 to G1 phase, and expression of integrins a6 and b4, which form an adhesion receptor for extracellular matrix (ECM) components, are suppressed at both the transcriptional and translational levels.…”

New Insights into the Role of Podoplanin in Epithelial–Mesenchymal Transition

“…One is the increase in cellular motility driven by podoplanin interacting with the ERM proteins-Rho GTPases axis; the second is what we call "effective cohesiveness" in the cells. Effective cohesiveness designates a sensor mechanism or metabolite that would integrate the overall adhesive properties of a cell, and could be regulated by transcriptional and epigenetic mechanisms, protein stability, presence in the plasma membrane and context-specific mechanisms (like, for instance, the properties of the extracellular matrix) (Adhikary et al, 2014;Boulter et al, 2012;Engl et al, 2014;Friedl et al, 2014;Gueron et al, 2014;Marjoram et al, 2014;McGrail et al, 2014;Murali and Rajalingam, 2014;Orgaz et al, 2014;Sadok and Marshall, 2014;Thiery et al, 2012;50 Zegers and Friedl, 2014). If podoplanin is expressed in a cellular context with low effective cohesiveness, it will promote mesenchymal motility that will end up in EMT.…”

Aspirin Suppresses the Acquisition of Chemoresistance in Breast Cancer by Disrupting an NFκB–IL6 Signaling Axis Responsible for the Generation of Cancer Stem Cells

“…Primers are listed in Supplementary Table S2. The PCR products were analyzed by 2% agarose gel electrophoresis (32,33).…”