Functional Role of Arginine During the Peri-implantation Period of Pregnancy. II. Consequences of Loss of Function of Nitric Oxide Synthase NOS3 mRNA in Ovine Conceptus Trophectoderm1

Wang, Xiaoqiu; Frank, J.; Xu, Jing; Dunlap, Kathrin A.; Satterfield, M. Carey; Burghardt, Robert C.; Romero, Jared J.; Hansen, Thomas; Wu, Guoyao

doi:10.1095/biolreprod.114.121202

Cited by 31 publications

(17 citation statements)

References 47 publications

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“…Nitric oxide synthase 3 has been shown to be a key enzyme for NO production in ovine TE. Knockdown by using an in vivo morpholino antisense oligonucleotide for NOS3 resulted in thin, small and under developed conceptuses (Wang et al 2014b). These conceptuses also had decreased SLC7A1 message in the TE as well.…”

Section: Discussionmentioning

confidence: 99%

Arginine increases development of in vitro-produced porcine embryos and affects the protein arginine methyltransferase–dimethylarginine dimethylaminohydrolase–nitric oxide axis

Redel¹,

Tessanne²,

Spate³

et al. 2015

Reprod. Fertil. Dev.

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Culture systems promote development at rates lower than the in vivo environment. Here, we evaluated the embryo’s transcriptome to determine what the embryo needs during development. A previous mRNA sequencing endeavor found SLC7A1, an arginine transporter, to be up-regulated in in vitro compared to in vivo cultured embryos. We added different concentrations of arginine to our culture medium to meet the needs of the embryo. Increasing arginine from 0.12 to 1.69 mM improved the number of embryos that developed to the blastocyst stage. These blastocysts also had more total nuclei compared to controls and specifically, more trophectoderm nuclei. Embryos cultured in 1.69 mM arginine had lower SLC7A1 levels and had higher abundance of messages involved with glycolysis (HK1, HK2, and GPT2) and decreased expression of genes involved with blocking the TCA cycle (PDK1) as well as the pentose phosphate pathway (TALDO1). PRMT1, PRMT3 and PRMT5 expression throughout development was not affected by arginine. DDAH1 and DDAH2 message was found to be differentially regulated through development and DDAH2 protein was localized to the nuclei of blastocysts. Arginine has a positive effect on preimplantation development and may be affecting the NO-DDAH-PRMT axis.

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Section: Discussionmentioning

confidence: 99%

Arginine increases development of in vitro-produced porcine embryos and affects the protein arginine methyltransferase–dimethylarginine dimethylaminohydrolase–nitric oxide axis

Redel¹,

Tessanne²,

Spate³

et al. 2015

Reprod. Fertil. Dev.

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“…NO, CO, and H 2 S) to regulate nutrient metabolism. 2,18,26,27 For example, dietary arginine enhances the abundance of phosphorylated mammalian target of the rapamycin (mTOR), eukaryotic initiation factor (eIF) 4E-binding protein-1 (4E-BP1), and ribosomal protein S6 kinase 1 (S6K1), as well as the formation of the active eIF4E-eIF4G complex, but reduces the abundance of the inactive 4E-BP1-eIF4E complex in skeletal muscle, leading to increased protein synthesis and whole-body growth. 28 In addition, dietary glutamine enhances intestinal integrity, cell survival, and villus height in association with activation of mTOR cell signaling, while attenuating weaning-induced reduction in occludin, claudin-1, zonula occluden (ZO)-2, and ZO-3 protein abundances.…”

Section: Regulation Of Cell Signaling Pathwaysmentioning

confidence: 99%

Dietary essentiality of “nutritionally non-essential amino acids” for animals and humans

Hou

Yin

2015

Exp Biol Med (Maywood)

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227

127

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Based on growth or nitrogen balance, amino acids (AA) had traditionally been classified as nutritionally essential (indispensable) or non-essential (dispensable) for animals and humans. Nutritionally essential AA (EAA) are defined as either those AA whose carbon skeletons cannot be synthesized de novo in animal cells or those that normally are insufficiently synthesized de novo by the animal organism relative to its needs for maintenance, growth, development, and health and which must be provided in the diet to meet requirements. In contrast, nutritionally non-essential AA (NEAA) are those AA which can be synthesized de novo in adequate amounts by the animal organism to meet requirements for maintenance, growth, development, and health and, therefore, need not be provided in the diet. Although EAA and NEAA had been described for over a century, there are no compelling data to substantiate the assumption that NEAA are synthesized sufficiently in animals and humans to meet the needs for maximal growth and optimal health. NEAA play important roles in regulating gene expression, cell signaling pathways, digestion and absorption of dietary nutrients, DNA and protein synthesis, proteolysis, metabolism of glucose and lipids, endocrine status, men and women fertility, acid-base balance, antioxidative responses, detoxification of xenobiotics and endogenous metabolites, neurotransmission, and immunity. Emerging evidence indicates dietary essentiality of ''nutritionally non-essential amino acids'' for animals and humans to achieve their full genetic potential for growth, development, reproduction, lactation, and resistance to metabolic and infectious diseases. This concept represents a new paradigm shift in protein nutrition to guide the feeding of mammals (including livestock), poultry, and fish.

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“…In vivo Arg-treatment enhanced placental functions during advanced stages of pregnancy in sheep [46][47][48]71,73]. Furthermore, in vitro studies have demonstrated the importance of Arg for uterine and conceptus growth and functions during the peri-implantation period of pregnancy in sheep [74][75][76]. However, very little is known about potential Arg effects on any reproductive processes, including uterine functions in nonpregnant animals.…”

Section: Revised 12mentioning

confidence: 99%

Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

et al. 2017

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The aim of this study was to evaluate lipid droplet (LD) expression in uteri of FSH-treated or nontreated sheep administered with arginine (Arg) or vehicle (saline, Sal) and fed a control (C), excess (overfed, O) or restricted (underfed, U) diet. In experiment 1, ewes from each diet were randomly assigned to Arg or Sal treatments administered three times daily starting on Day 0 of the first estrous cycle until blood sample and uterine tissue collection at the early- or mid-luteal phase of the second estrous cycle or the late-luteal phase of the first estrous cycle. In experiment 2, ewes were injected twice daily with FSH on Days 13 to 15 of the first estrous cycle, and blood samples and uterine tissue were collected at the early- and mid-luteal phases of the second estrous cycle. Cryopreserved in optimum cutting temperature (OCT) compound, cross-sections of uterine horn were stained with boron-dipyrromethene (BODIPY; marker of LDs) followed by 4',6-diamidino-2-phenylindole (DAPI) staining and image analysis to determine the proportion (%) of area occupied by LD in luminal epithelium (LE) and endometrial glands (EGs). Control ewes maintained, O ewes gained, and U ewes lost body weight during the experiments. Serum progesterone concentration was not affected by nutritional plane or Arg treatment and was 5.5-fold greater in FSH- than Sal-treated ewes. LDs were detected in LE and superficial EG (close to LE) but not deep EG, or other uterine compartments, and area occupied by LD was greater in LE than in EG. In experiment 1, in LE and EG, area occupied by LDs was greater in C than in O or U; greater in Arg than in Sal; and greater at the late-, less at mid-, and least at early-luteal phases. In experiment 2, in LE and EG, area occupied by LDs was greater at mid- than in early-luteal phase. Comparison of data from FSH-treated and nontreated ewes (e.g., experiment 1 vs. experiment 2) demonstrated that FSH increased the area occupied by LD in LE and EG regardless of diet. Interactions between FSH treatment, stage of the estrous cycle, and plane of nutrition demonstrated that FSH increased the area occupied by LD in LE and EG at the mid-luteal phase in O and U. Thus, LDs are differentially distributed in uterine compartments, and area occupied by LD in endometrium is affected by nutritional plane, Arg or FSH, and stage of the estrous cycle. Such changes in dynamics of LD in the endometrium during the estrous cycle indicate their specific role in uterine functions.

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Functional Role of Arginine During the Peri-implantation Period of Pregnancy. II. Consequences of Loss of Function of Nitric Oxide Synthase NOS3 mRNA in Ovine Conceptus Trophectoderm1

Cited by 31 publications

References 47 publications

Arginine increases development of in vitro-produced porcine embryos and affects the protein arginine methyltransferase–dimethylarginine dimethylaminohydrolase–nitric oxide axis

Arginine increases development of in vitro-produced porcine embryos and affects the protein arginine methyltransferase–dimethylarginine dimethylaminohydrolase–nitric oxide axis

Dietary essentiality of “nutritionally non-essential amino acids” for animals and humans

Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

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