Standardization of renal function evaluation in Wistar rats (<i>Rattus norvegicus</i>) from the Federal University of Juiz de Fora's colony

Castro, Bárbara Bruna Abreu de; Colugnati, Fernando Antônio Basile; Cenedeze, Marcos Antônio; Suassuna, Paulo Giovanni de Albuquerque; Sanders-Pinheiro, Hélady

doi:10.5935/0101-2800.20140023

Cited by 25 publications

(16 citation statements)

References 24 publications

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“…Moreover, renal I/R resulted in a significant increase in FE Na+ on the 2 time points of follow-up after ischaemia, suggesting a significant impairment of tubular function. These findings confirm that I/R injury to the kidney causes both glomerular and tubular dysfunctions, and are in agreement with those reported by others (Bussmann et al, 2014;Castro et al, 2014;Malek and Nematbakhsh, 2015;Punuru et al, 2014). Renal I/R injury is characterized by decreases in the glomerular filtration rate (GFR), tubular and glomerular damage, impairment in hemodynamic regulation and energy depletion (Malek and Nematbakhsh, 2015).…”

Section: Discussionsupporting

confidence: 91%

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Elsaid¹,

Khalil²,

Ibrahim³

et al. 2019

Acta Sci Pol Technol Aliment

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Background. The present work was designed to study the effects of methanolic stevia extracts and aerobic exercise and combination of both on renal I/R injury in male rats. Methods. 60 adult male Sprague-Dawley rats were subdivided into five equal groups as sham, control, exercise, stevia, and stevia plus exercise group. After 5 weeks of exercise and stevia, animals were exposed to 45 min of left renal ischemia and right nephrectomy followed by reperfusion. Serum creatinine, creatinine clearance, fractional Na excretion (FE Na +), malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT) levels in kidney tissues were measured. Also, renal histopathology and the expression of caspase-3 by immunohistochemical examination were done. Results. The results showed that stevia, exercise or combination of stevia and exercise caused a significant decrease in serum level of creatinine (p < 0.001) and FE Na + (p < 0.001) and an increase in creatinine clearance (p < 0.001). Moreover, this caused a significant decrease in (MDA; p < 0.046) and an increase in GSH (p < 0.01) and CAT (p < 0.01), as well as causing a significant decrease in caspase 3 expression compared to the control group. Conclusion. Pretreatment with either stevia or exercise of combination of both seem to have protective effects on renal I/R injury. However, the protective effect of exercise against renal I/R injury seems to be less than stevia. These effects might be due to attenuation of oxidative stress and apoptosis in kidney tissues.

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Section: Discussionsupporting

confidence: 91%

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Elsaid¹,

Khalil²,

Ibrahim³

et al. 2019

Acta Sci Pol Technol Aliment

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“…The glomerular filtration rate (GFR) is a well-known tool to measure the excretory capacity of the kidney. In clinical practice, GFR is obtained from the creatinine clearance in urine samples collected over 24 hours [32]. The significantly higher creatinine clearance rate in the EL 200 (mid dose) and EL 400 (high dose) groups compared to PCM alone group demonstrated the ability of EL to eliminate creatinine from blood into urine, eventually normalising creatinine content in the blood.…”

Section: Discussionmentioning

confidence: 99%

Nephroprotective Effect of Herbal Extract Eurycoma longifolia on Paracetamol-Induced Nephrotoxicity in Rats

Chinnappan

George

Thaggikuppe³

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Paracetamol (PCM) is a well-known drug widely used for its analgesic and antipyretic properties. PCM is generally considered as safe but overdose of PCM can cause nephrotoxicity. Traditionally, herbs have been used for the treatment of drug or toxin-induced renal disorders and numerous medicinal plants were tested for nephroprotection effect in PCM-induced nephrotoxicity model. The aim of the present study was to evaluate the protective effect of the herbal extract Eurycoma longifolia (EL) against PCM-induced nephrotoxicity rat model. Forty Wistar rats were randomly divided into five groups of eight rats each: control (vehicle 10 ml/kg), PCM alone (200 mg/kg PCM), EL 100 (EL 100 mg/kg+200 mg/kg PCM), EL 200 (EL 200 mg/kg+200 mg/kg PCM), and EL 400 (EL 400 mg/kg+200 mg/kg PCM). All animals from control group received vehicle daily and animals from groups PCM alone, EL 100, EL 200, and EL 400 received repeated dose of PCM and the assigned treatment of EL daily for a period of 14 days. On the 15th day, serum creatinine, blood urea nitrogen, protein, and albumin were measured in blood and creatinine clearance was measured in urine collected over 24 hours. Kidney sections of all experimental groups underwent histopathological analysis. There was a significant (p<0.05) increase in serum creatinine and blood urea levels in the PCM alone group compared to the treatment groups due to nephrotoxicity. In the treatment groups, there was a dose-dependent protection against PCM-induced changes observed in serum total protein, albumin, urea, and creatinine. Significant (p<0.05) drop was seen in serum creatinine and blood urea content in EL 200 and EL 400 groups. Creatinine clearance significantly increased for EL 200 (p<0.01) and EL 400 (p < 0.001) groups. Serum total protein and serum albumin content were significantly increased (p<0.05) in EL 200 and EL 400 groups compared to PCM alone group. Histopathological examination (H&E staining) of the rat kidneys revealed severe degeneration in the PCM alone group, while there was evidence of significant dose-dependent protection in the treatment groups against PCM-induced changes. The serum and urine biochemical results and histopathology analysis of the kidney indicate the nephroprotective potential of EL extract against PCM-induced nephrotoxicity.

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“…Creatinine was measured in urine and serum by Enzymatic Method (Creatinina K kit, Labtest Diagnóstico, Lagoa Santa, Brazil), which is based on Jaffe's reaction principle associated to the application of a correction index (8). This methodology minimizes the interference on creatinine determination.…”

Section: Creatinine Clearancementioning

confidence: 99%

Alternative pathways for angiotensin II production as an important determinant of kidney damage in endotoxemia

Rosa

Colucci

Yokota

et al. 2016

American Journal of Physiology-Renal Physiology

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Sepsis is an uncontrolled systemic inflammatory response against an infection and a major public health issue worldwide. This condition affects several organs, and, when caused by Gram-negative bacteria, kidneys are particularly damaged. Due to the importance of renin-angiotensin system (RAS) in regulating renal function, in the present study, we aimed to investigate the effects of endotoxemia over the renal RAS. Wistar rats were injected with Escherichia coli lipopolysaccharide (LPS) (4 mg/kg), mimicking the endotoxemia induced by Gram-negative bacteria. Three days after treatment, body mass, blood pressure, and plasma nitric oxide (NO) were reduced, indicating that endotoxemia triggered cardiovascular and metabolic consequences and that hypotension was maintained by NO-independent mechanisms. Regarding the effects in renal tissue, inducible NO synthase (iNOS) was diminished, but no changes in the renal level of NO were detected. RAS was also highly affected by endotoxemia, since renin, angiotensin-converting enzyme (ACE), and ACE2 activities were altered in renal tissue. Although these enzymes were modulated, only angiotensin (ANG) II was augmented in kidneys; ANG I and ANG 1-7 levels were not influenced by LPS. Cathepsin G and chymase activities were increased in the endotoxemia group, suggesting alternative pathways for ANG II formation. Taken together, our data suggest the activation of noncanonical pathways for ANG II production and the presence of renal vasoconstriction and tissue damage in our animal model. In summary, the systemic administration of LPS affects renal RAS, what may contribute for several deleterious effects of endotoxemia over kidneys.

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Standardization of renal function evaluation in Wistar rats (Rattus norvegicus) from the Federal University of Juiz de Fora's colony

Abstract: Was standardized: 24-hour urine collection without fasting. The deproteinization showed no benefit. The measurements were performed with spectrophotometer reliability. It generated a practical formula for estimating PT 24 hours through rP/C.

Cited by 25 publications

References 24 publications

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats [pdf]

Nephroprotective Effect of Herbal Extract Eurycoma longifolia on Paracetamol-Induced Nephrotoxicity in Rats

Alternative pathways for angiotensin II production as an important determinant of kidney damage in endotoxemia

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