Epitope mapping of the infectious hematopoietic necrosis virus glycoprotein by flow cytometry

Xu, Liming; Liu, Miao; Zhao, Juanjuan; Cao, Yanghui; Yin, Jing; Liu, Hongbai; Lü, Tao

doi:10.1007/s10529-014-1586-2

Cited by 12 publications

(4 citation statements)

References 15 publications

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“…The Ja serotype of IPNV ChRtm213 33 and the J genotype IHNV Sn1203 32 are laboratory stocks. IPNV ChRtm213 was propagated in CHSE-214 cells and IHNV Sn1203 was propagated in EPC cells, as described previously 16, 51 .…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have demonstrated the co-infection of rainbow trout with IHNV and IPNV under natural conditions 30, 31 . Therefore, in this study, a bivalent DNA vaccine was constructed with the G gene of Chinese IHNV isolate Sn1203 32 and the VP2–VP3 genes of Chinese IPNV isolate ChRtm213 33 . Here, we report the successful design and construction of this bivalent DNA vaccine, designated pCh-IHN/IPN, which induced protective immune responses against IHNV infection, IPNV infection, and co-infection with IHNV and IPNV in the rainbow trout.…”

Section: Introductionmentioning

confidence: 99%

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Bivalent DNA vaccine induces significant immune responses against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus in rainbow trout

Zhao

Liu

et al. 2017

Sci Rep

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Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens of salmon and trout. An active bivalent DNA vaccine was constructed with the glycoprotein gene of Chinese IHNV isolate Sn1203 and VP2–VP3 gene of Chinese IPNV isolate ChRtm213. Rainbow trout (5 g) were vaccinated by intramuscular injection with 1.0 µg of the bivalent DNA vaccine and then challenged with an intraperitoneal injection of IHNV, IPNV, or both, at 30 and 60 days post-vaccination (d.p.v.). High protection rates against IHNV were observed, with 6% and 10% cumulative mortality, respectively, compared with 90–94% in the mock-vaccinated groups. IPNV loads (531-fold and 135-fold, respectively) were significantly reduced in the anterior kidneys of the vaccinated trout. Significant protection against co-infection with IHNV and IPNV was observed, with cumulative mortality rates of 6.67% and 3.33%, respectively, compared with 50.0% and 43.3%, respectively, in the mock-vaccinated groups. No detectable infective IHNV or IPNV was recovered from vaccinated trout co-infected with IHNV and IPNV. The bivalent DNA vaccine increased the expression of Mx-1 and IFN-γ at 4, 7, and 15 d.p.v, and IgM at 21 d.p.v., and induced high titres (≥160) of IHNV and IPNV neutralizing antibodies at 30 and 60 d.p.v.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bivalent DNA vaccine induces significant immune responses against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus in rainbow trout

Zhao

Liu

et al. 2017

Sci Rep

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“…Several approaches have been used for the search of immunogenic proteins for viral and bacterial vaccines. In the case of viral vaccines, reverse genetics have been used for the identification of immunogenic proteins for IPNV [ 74 ], peptide scanning for NNV [ 75 ] and IPNV [ 76 ], phage display for infectious salmon anemia virus (ISAV) [ 77 ] and IHNV [ 78 ] while bioinformatics has been used to identify immunogenic proteins for SGIV [ 79 ]. The search of bacterial immunogenic proteins is mostly centered on identifying antigens that provide protective immunity across variant serotypes of the same bacterial species.…”

Section: Application Of Genomic Studies In Vaccine Developmentmentioning

confidence: 99%

Teleosts Genomics: Progress and Prospects in Disease Prevention and Control

Munang’andu

Galindo-Villegas

David

2018

IJMS

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Genome wide studies based on conventional molecular tools and upcoming omics technologies are beginning to gain functional applications in the control and prevention of diseases in teleosts fish. Herein, we provide insights into current progress and prospects in the use genomics studies for the control and prevention of fish diseases. Metagenomics has emerged to be an important tool used to identify emerging infectious diseases for the timely design of rational disease control strategies, determining microbial compositions in different aquatic environments used for fish farming and the use of host microbiota to monitor the health status of fish. Expounding the use of antimicrobial peptides (AMPs) as therapeutic agents against different pathogens as well as elucidating their role in tissue regeneration is another vital aspect of genomics studies that had taken precedent in recent years. In vaccine development, prospects made include the identification of highly immunogenic proteins for use in recombinant vaccine designs as well as identifying gene signatures that correlate with protective immunity for use as benchmarks in optimizing vaccine efficacy. Progress in quantitative trait loci (QTL) mapping is beginning to yield considerable success in identifying resistant traits against some of the highly infectious diseases that have previously ravaged the aquaculture industry. Altogether, the synopsis put forth shows that genomics studies are beginning to yield positive contribution in the prevention and control of fish diseases in aquaculture.

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“…Infectious hematopoietic necrosis (IHN) is an acute infectious disease that affects fishes and is caused by the infectious hematopoietic necrosis virus (IHNV), which is highly common in salmonids. IHN can cause 90 to 100% mortality in rainbow trout ( Oncorhynchus mykiss ) depending on differences in host species, virus strains, and environmental conditions [ 8 , 9 ]. Based on the significant economic losses caused by infectious hematopoietic necrosis to the global rainbow trout aquaculture industry each year, the World Organization for Animal Health (OIE) listed it as an animal disease that must be declared during import and export.…”

Section: Introductionmentioning

confidence: 99%

Andrographolide Alleviates Oxidative Damage and Inhibits Apoptosis Induced by IHNV Infection via CTSK/BCL2/Cytc Axis

Liu,

Li,

Zhao

et al. 2023

IJMS

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Infectious hematopoietic necrosis virus (IHNV) is an important pathogen that causes significant economic losses to salmon trout farming. Although vaccines have been invented for the treatment of IHNV, findings from our previous survey show that breeding enterprises and farmers require effective oral drugs or immune enhancers. However, studies on the development of oral drugs are limited. In the present study, we used bioinformatics methods to predict the protein targets of andrographolide (Andro) in IHNV. Cells were infected with IHNV, and the effect of andrographolide was explored by evaluating the expression levels of genes implicated in oxidative stress, activities of antioxidant enzymes, and the expression of genes implicated in apoptosis and necrosis. In the present study, cells were divided into NC, IHNV, IHNV+10 μM andrographolide, and IHNV+20 μM andrographolide groups. qRT-PCR was performed to determine the expression level of genes, and an antioxidant enzyme detection kit was used to evaluate the activities of antioxidant enzymes. Fluorescent staining was performed using a reactive oxygen species detection kit (ROS) and Hoechst 33342/PI double staining kit, and the mechanism of alleviation of apoptosis and oxidative stress andrographolide after IHNV infection was determined. The results indicated that andrographolide inhibits viral growth by binding to the NV protein of IHNV and increasing the antioxidant capacity of the body through the CTSK/BCL2/Cytc axis, thereby inhibiting the occurrence of IHNV-induced apoptosis. This is the first study to explore the antagonistic mechanism of action of andrographolide in alleviating IHNV infection. The results provide valuable information on alternative strategies for the treatment of IHNV infection during salmon family and provide a reference for the use of andrographolide as an antioxidant agent in agricultural settings.

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Epitope mapping of the infectious hematopoietic necrosis virus glycoprotein by flow cytometry

Cited by 12 publications

References 15 publications

Bivalent DNA vaccine induces significant immune responses against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus in rainbow trout

Bivalent DNA vaccine induces significant immune responses against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus in rainbow trout

Teleosts Genomics: Progress and Prospects in Disease Prevention and Control

Andrographolide Alleviates Oxidative Damage and Inhibits Apoptosis Induced by IHNV Infection via CTSK/BCL2/Cytc Axis

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