Determination by UPLC-MS of four dammarane-type saponins from heat-processed <i>Gynostemma pentaphyllum</i>

Wu, Qian; Jang, Moonhee; Piao, Xiang‐Lan

doi:10.1080/09168451.2014.882751

Cited by 17 publications

(12 citation statements)

References 21 publications

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“…Gyp-L has been demonstrated to act as a potential activators of AMP-activated protein kinase [35, 36]. Previous study by Piao et al has simply explored the anticancer activity of Gyp-L in human lung cancer cells [37].…”

Section: Discussionmentioning

confidence: 99%

Gypenoside L inhibits autophagic flux and induces cell death in human esophageal cancer cells through endoplasm reticulum stress-mediated Ca2+ release

Liao

Zheng

et al. 2016

Oncotarget

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Esophageal cancer is one of the leading cause of cancer mortality in the world. Due to the increased drug and radiation tolerance, it is urgent to develop novel anticancer agent that triggers nonapoptotic cell death to compensate for apoptosis resistance. In this study, we show that treatment with gypenoside L (Gyp-L), a saponin isolated from Gynostemma pentaphyllum, induced nonapoptotic, lysosome-associated cell death in human esophageal cancer cells. Gyp-L-induced cell death was associated with lysosomal swelling and autophagic flux inhibition. Mechanistic investigations revealed that through increasing the levels of intracellular reactive oxygen species (ROS), Gyp-L triggered protein ubiquitination and endoplasm reticulum (ER) stress response, leading to Ca2+ release from ER inositol trisphosphate receptor (IP3R)-operated stores and finally cell death. Interestingly, there existed a reciprocal positive-regulatory loop between Ca2+ release and ER stress in response to Gyp-L. In addition, protein synthesis was critical for Gyp-L-mediated ER stress and cell death. Taken together, this work suggested a novel therapeutic option by Gyp-L through the induction of an unconventional ROS-ER-Ca2+-mediated cell death in human esophageal cancer.

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Section: Discussionmentioning

confidence: 99%

Gypenoside L inhibits autophagic flux and induces cell death in human esophageal cancer cells through endoplasm reticulum stress-mediated Ca2+ release

Liao

Zheng

et al. 2016

Oncotarget

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“…The ginsenoside standards used in this study were isolated and purified from P. ginseng roots and red ginseng by a series of chromatography procedures in our laboratory, and the information for 30 ginsenosides was reported in our previous study [ 25 ]. The structures of other ginsenosides were determined based on the spectroscopic data (MS, hydrogen nuclear magnetic resonance ( 1 H-NMR), and carbon-13 ( 13 C)-NMR) in the previous literatures: fKa [ 26 ], nR4 [ 27 ], nR2-R [ 28 ], Rh1-R [ 29 ], Ra3 [ 26 ], M-Rb1 [ 30 ], M-Rc [ 31 ], M-Rb2 [ 30 ], M-Rd [ 31 ], CO [ 32 ], GyL [ 33 ], GyLI [ 33 ], nFt1 [ 34 ], Rg3-R [ 29 ], Mc [ 35 ], and CY [ 35 ]. Rf, Rk1, Ro, Rg2-R, PPD, and PPT were purchased from Sigma Aldrich (St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS

et al. 2017

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The effective production and usage of ginsenosides, given their distinct pharmacological effects, are receiving increasing amounts of attention. As the ginsenosides content differs in different parts of Panax ginseng, we wanted to assess and compare the ginsenosides content in the ginseng roots, leave, stems, and berries. To extract the ginsenosides, 70% (v/v) methanol was used. The optimal ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) method was used to profile various ginsenosides from the different parts of P. ginseng. The datasets were then subjected to multivariate analysis including principal component analysis (PCA) and hierarchical clustering analysis (HCA). A UPLC-QTOF/MS method with an in-house library was constructed to profile 58 ginsenosides. With this method, a total of 39 ginsenosides were successfully identified and quantified in the ginseng roots, leave, stem, and berries. PCA and HCA characterized the different ginsenosides compositions from the different parts. The quantitative ginsenoside contents were also characterized from each plant part. The results of this study indicate that the UPLC-QTOF/MS method can be an effective tool to characterize various ginsenosides from the different parts of P. ginseng.

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“…Then, internal standard protopanaxatriol was added to each solution. The various saponins in total saponin were then calculated and quantified by utilizing a formula as described by Wu et al [ 34 ]. The HPLC-MS analysis of flavonoids is similar to saponins; kaempferol (Sigma, USA) and rutin (Sigma, USA) were used as the flavonoid standards for analysis.…”

Section: Methodsmentioning

confidence: 99%

Gynostemma pentaphyllum Attenuates the Progression of Nonalcoholic Fatty Liver Disease in Mice: A Biomedical Investigation Integrated with In Silico Assay

Hong

Cai

Song

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Nonalcoholic fatty liver disease (NAFLD) is the most common type of liver disease in developed countries. Oxidative stress plays a critical role in the progression of NAFLD. Modern pharmacological study and clinical trials have demonstrated the remarkable antioxidant activity of Gynostemma pentaphyllum (GP) in chronic liver disease. One aim of this study was to explore the potential protective effects and mechanisms of action of GP extract on NAFLD. The in vivo results showed that GP extract could alleviate fatty degeneration and haptic fibrosis in NAFLD mice. For exploring the hepatoprotective mechanisms of GP, we used network pharmacology to predict the potential active components of GP and their intracellular targets in NAFLD. Based on the network pharmacology results, we further utilized biomedical assays to validate this in silico prediction. The results showed that Gypenoside XL could upregulate the protein level of PPARα in NAFLD; the transcription level of several PPARα downstream target genes such as acyl-CoA oxidase (ACO) and carnitine palmitoyltransferase-1 (CPT-1) also increased after Gypenoside XL treatment. The overexpression of ACO and CPT-1 may involve the hepatoprotective effects of GP and Gypenoside XL on NAFLD by regulating mitochondrial fatty acid β-oxidation.

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Determination by UPLC-MS of four dammarane-type saponins from heat-processed Gynostemma pentaphyllum

Cited by 17 publications

References 21 publications

Gypenoside L inhibits autophagic flux and induces cell death in human esophageal cancer cells through endoplasm reticulum stress-mediated Ca2+ release

Gypenoside L inhibits autophagic flux and induces cell death in human esophageal cancer cells through endoplasm reticulum stress-mediated Ca2+ release

Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS

Gynostemma pentaphyllum Attenuates the Progression of Nonalcoholic Fatty Liver Disease in Mice: A Biomedical Investigation Integrated with In Silico Assay

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