Associated changes in the transcription levels of IL-17A and tight junction-associated genes in the duodenal mucosa of rhesus macaques repeatedly exposed to simian/human immunodeficiency virus

Zhang, Wenjun; Wang, Yue; Yu, Kai; Duan, Jia-Zhong; Yao, Wenrong; Wang, Yan; Yang, Rongge; Yang, Guan‐Wen

doi:10.1016/j.yexmp.2014.07.007

Cited by 7 publications

(9 citation statements)

References 52 publications

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“…, Group I) were compared with that from SHIV negative rhesus macaques (Fig. , Group II) . As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…All the tissues used in this study were archived frozen tissues from rhesus macaques ( Macaca mulatta ) of Chinese origin. Tissues from the gastrointestinal (GI) tract including biopsies from duodenal mucosa of 20 rhesus macaques 2 weeks before and 10 days after repeated rectal inoculations with SHIV‐SF162P4 , and necropsy samples of duodenum, jejunum, ileum, caecum, colon and rectum from 5 normal rhesus macaques and 5 SHIV/SIV chronically infected rhesus macaques were examined . The rhesus macaques for necropsy samples had been inoculated repeatedly with SHIV‐SF162P4 via rectal route as described previously , and later with a single dose of SHIV‐SF162P4 and SIV mac251 intravenously.…”

Section: Methodsmentioning

confidence: 99%

“…However, knowledge of the rhesus macaque immune system is limited and the expression of pIgR and FcRn in these animals has not been well addressed. Previously, IL‐17A transcription levels were found altered in the duodenal mucosa of SHIV‐infected rhesus macaques . In addition, it was found that Th17 cells could enhance pIgR expression in mice , and IL‐17 could induce pIgR expression and IgA transcytosis .…”

Section: Introductionmentioning

confidence: 99%

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Alteration of Polymeric Immunoglobulin Receptor and Neonatal Fc Receptor Expression in the Gut Mucosa of Immunodeficiency Virus‐Infected Rhesus Macaques

Wang¹,

Yang

2016

Scand J Immunol

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Polymeric immunoglobulin receptors (pIgR) and neonatal Fc receptors (FcRn) are crucial immunoglobulin (Ig) receptors for the transcytosis of immunoglobulins, that is IgA, IgM and IgG, the levels of which in mucosal secretions were altered in both HIV-and SIV-infected individuals. To gain an insight into the changes of pIgR and FcRn expression after immunodeficiency virus (SHIV/SIV) infection, real-time RT-PCR methods were established and the mRNA levels of pIgR and FcRn in normal and SHIV/SIV-infected rhesus macaques were quantitatively examined. It was found that the levels of pIgR mRNA were within a range of 10 7 copies per million copies of GAPDH mRNA in the gut mucosa of rhesus macaques, which were up to 55 times higher than that in the oral mucosa, the highest among the non-gut tissues examined. Levels of FcRn mRNA were generally lower than that of pIgR, and the levels of FcRn mRNA in the gut mucosa were also lower than that in most non-gut tissues examined.

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“…, Group I) were compared with that from SHIV negative rhesus macaques (Fig. , Group II) . As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Alteration of Polymeric Immunoglobulin Receptor and Neonatal Fc Receptor Expression in the Gut Mucosa of Immunodeficiency Virus‐Infected Rhesus Macaques

Wang¹,

Yang

2016

Scand J Immunol

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“…In our previous study indicated that complex interactions occur between diverse types of enteroendocrine cells and various immune cells through paracrine mechanisms or via mechanisms dependent on cell-to-cell contact; such interactions might play key roles in maintaining the gut mucosal barrier integrity of rhesus macaques [40]. Then our another new study revealed that acute simian/human immunodeficiency viruses (SHIV), and by extension HIV infection could affect the expression of gastrointestinal tract epithelial tight junction associated genes, probably through IL-17A and other immune alterations [41]. These two studies talked about integrity of the gut mucosal barrier, which had effect on microbial translocation.…”

Section: Microbial Translocation and Immune Active In Hiv/siv Infectionmentioning

confidence: 94%

Can We Control the Progression of HIV by Monitor Markers of Microbial Translocation?

Zhang¹,

Guo²

2016

J Antivir Antiretrovir

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This review summarizes microbial translocation in HIV infection. Microbial translocation can be measured by bacterial products in plasma, such as LPS and bacterial DNA or RNA fragments, or indirectly by LBP, sCD14, EndoCAb, and antiflagellin antibodies. In some study, these markers had contrary results. May be microbial translocation is not the sole driver of HIV progression. Several lines of research indicated that a major contributor to immune activation and disease progression during HIV infection was microbial translocation. Although successful treatment with ART increased GALT CD4 + T cells and suppresses HIV RNA, the numbers of these cells did not return to prior levels. There is a negative effect of mucosal immune dysfunction and microbial translocation on HIV disease progression in the presence of ART. The topic of microbial translocation and immune activation in HIV infection still is a research focus.

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“…The total RNA for real‐time RT‐PCR was extracted from the thymus, spleen, inguinal lymph node, tonsil, skin, trachea, lung, heart, liver, kidney, pancreas, vagina, oral cavity, proximal duodenum, proximal jejunum, distal ileum, caecum, proximal colon, and rectum of Chinese rhesus macaques. Total RNA from 10–20 mg of each type of tissues or 10 7 PBMCs was extracted with RNAPrep Pure Tissue Kit (Tiangen, Beijing, China) as described previously . Extracted RNA was eluted with 100 μl RNase‐Free water and divided into small aliquots and stored at −80°.…”

Section: Methodsmentioning

confidence: 99%

Molecular characteristics of rhesus macaque interleukin‐22: cloning, in vitro expression and biological activities

Wang

Cui

et al. 2018

Immunology

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Interleukin-22 (IL-22) is a potential therapeutic agent for diseases driven by epithelial injury. To characterize the IL-22 expressed by rhesus macaques, animals that are irreplaceable for human disease research, rhesus macaque IL-22 (rhIL-22) was cloned and expressed, and its biological activity and in vivo distribution were examined. It was found that the rhIL-22 gene consists of five introns and six exons, including a short non-coding exon starting 22 bp downstream of a putative TATA box. The amino acid sequence of rhIL-22 showed 95·5% identity to that of humans, and it shared two conserved disulphide bonds, three N-glycosylation sites and all the critical residues for binding to IL-22R1. High levels of IL-22 mRNA were observed in the liver, pancreas, lymphoid tissues and especially in the outer-body barriers such as the intestinal tract of rhesus macaques. Functionally, purified rhIL-22 has a similar but a little earlier effect on signal transducer and activator of transcription 3 phosphorylation at Tyr705 compared with that of commercial human IL-22. The expression of the antibacterial proteins β-defensin-2, S100A8, S100A9, RegIIIα and Muc1 by HT-29 cells was largely upregulated after stimulation with rhIL-22. Recombinant rhIL-22 could also significantly promote the proliferation of human intestinal epithelial cells without affecting cell apoptosis. These data indicate that rhesus macaque IL-22 is highly similar to that of humans in both structure and function, and tests of therapeutic effects of human IL-22 on human diseases in rhesus macaques are warranted.

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Associated changes in the transcription levels of IL-17A and tight junction-associated genes in the duodenal mucosa of rhesus macaques repeatedly exposed to simian/human immunodeficiency virus

Cited by 7 publications

References 52 publications

Alteration of Polymeric Immunoglobulin Receptor and Neonatal Fc Receptor Expression in the Gut Mucosa of Immunodeficiency Virus‐Infected Rhesus Macaques

Alteration of Polymeric Immunoglobulin Receptor and Neonatal Fc Receptor Expression in the Gut Mucosa of Immunodeficiency Virus‐Infected Rhesus Macaques

Can We Control the Progression of HIV by Monitor Markers of Microbial Translocation?

Molecular characteristics of rhesus macaque interleukin‐22: cloning, in vitro expression and biological activities

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