Determination of a deuterohemin–peptide conjugate in rat plasma by liquid chromatography–tandem mass spectrometry and application to a preclinical pharmacokinetic study

Wang, Hao; Sun, Yan; Guo, Wei; Fang, Chunxue; Fawcett, J. Paul; Li, Wei; Gao, Yin; Yang, Yan; Gu, Jingkai

doi:10.1016/j.jpba.2014.06.026

Cited by 11 publications

(4 citation statements)

References 22 publications

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“…[6][7][8][9] It is appropriate to mention the measurement of the steady-state level of ROS-modified products by liquid chromatography coupled with mass spectrometry (LC/MS) identification. [33][34][35] The preceding paragraphs demonstrated that serious attention has to be paid to the problem of stability of ROMS in biological systems. Indeed, recognition of ROS homeostasis as a dynamic process may help to understand better the functioning of living organisms.…”

Section: Essential Biomarkers Of Oxidative Stress To Be Used For Clasmentioning

confidence: 99%

Time-course and intensity-based classifications of oxidative stresses and their potential application in biomedical, comparative and environmental research

Lushchak

2016

Redox Report

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Section: Essential Biomarkers Of Oxidative Stress To Be Used For Clasmentioning

confidence: 99%

Time-course and intensity-based classifications of oxidative stresses and their potential application in biomedical, comparative and environmental research

Lushchak

2016

Redox Report

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“…In addition, research on the extent of enantioselective binding of lansoprazole enantiomers to human serum proteins demonstrated that R-(+)lansoprazole was more tightly bound to serum proteins than S-(−)lansoprazole (Zhou, Yan, Pan, & Zeng, 2008). LC-MS/MS has became increasingly prevalent in biochemical analysis owing to its better sensitivity and selectivity, and its ability to provide high sample throughput (De et al, 2010;Wang et al, 2014). Better yet, R-(+)-lansoprazole elicited enhanced pharmacodynamics activity compared with racemic lansoprazole (Hotha et al, 2012;Kukulka, Eisenberg, & Nudurupati, 2011;Parekh, Oldfield, & Johnson, 2014 10-250 ng/mL) and the use of large volumes of plasma for intricate sample preparations (Cirilli et al, 2009;Jia et al, 2012;Miura, Tada, & Suzuki, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, the sample extraction of those methods was not ideal for high-throughput bioanalysis. LC-MS/MS has became increasingly prevalent in biochemical analysis owing to its better sensitivity and selectivity, and its ability to provide high sample throughput (De et al, 2010;Wang et al, 2014). Wang et al reported a column-switching LC-MS/MS method for the quantification of lansoprazole enantiomers in dog plasma, while the column-switching procedure required complicated and specific equipment (Wang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Enantioselective analysis of lansoprazole in rat plasma by LC–MS/MS: Application to a stereoselective pharmacokinetic study

Wen

et al. 2018

Biomedical Chromatography

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A rapid, sensitive and enantioselective method was developed and fully validated for the separation and determination of lansoprazole enantiomers in rat plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analytes and the internal standard (esomeprazole) were both extracted from plasma samples by liquid-liquid extraction with diethyl ether-dichloromethane (70:30; v/v). Satisfactory resolution (R = 2.0) was achieved within 7.3 min on a Chiralpak ID column (250 × 4.6 mm, 5 μm) employing acetonitrile-water (60:40, v/v) as the mobile phase at a flow rate of 0.6 mL/min. The acquisition of mass spectrometric data was performed in the multiple reaction monitoring mode coupled with a positive electrospray ionization source. A comprehensive validation of this method was rigorously conducted over the concentration range of 1.00-500.0 ng/mL for both enantiomers. All of the validation data demonstrated that the desirable linearity, sensitivity, accuracy, precision, recovery and stability were attained from the proposed approach. The established method was successfully applied to a stereoselective pharmacokinetic study of lansoprazole enantiomers in rat plasma after oral administration of 3 mg/kg racemic lansoprazole or dexlansoprazole. No chiral inversion was observed during the experimental procedure.

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“…Deuterohemin His peptide-6 (DhHP-6; Dh-β-AHTVEK-NH2), is a novel microperoxidase mimetic with a molecular weight of 1,230 Da. It has been demonstrated that DhHP-6 can enter cells and can exhibit high enzyme-activity to scavenge free radicals effectively ( 7 , 8 ). Furthermore, DhHP-6 has been observed to increase survival rates in Caenorhabditis elegans by promoting the elimination of oxidative stress ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

DhHP-6 attenuates cerebral ischemia-reperfusion injury in rats through the inhibition of apoptosis

Yan

et al. 2017

Molecular Medicine Reports

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As a novel reactive oxygen species (ROS) scavenger, deuterohemin His peptide-6 (DhHP-6) has been demonstrated to prolong the lifespan of Caenorhabditis elegans and has also exhibited protective effects in myocardial ischemia-reperfusion injury. Whether similar effects occur during cerebral ischemia-reperfusion (CIR) injury remains to be elucidated. The present study evaluated the function of DhHP-6 and its underlying mechanisms in a middle cerebral artery occlusion (MCAO) model in rats. The focal transient MCAO model was implemented using the Longa method of ischemia for 2 h followed by reperfusion for 22 h in male Wistar rats. DhHP-6 was administered at the onset of reperfusion via intraperitoneal injection. The infarct volume, brain edema, brain apoptosis and neurological function were evaluated 24 h following stroke. To further determine the role of DhHP-6 in CIR injury, the levels of ROS and malondialdehyde (MDA), the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), and the protein expression levels of B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax), cleaved caspase-3, cytochrome c, Bcl-2 and phosphorylated-Akt/Akt were measured in ischemic cortex tissues. The results demonstrated that DhHP-6 significantly improved infarct volume, brain edema and neurological deficits, and reduced the percentage of TUNEL-positive cells. The levels of ROS and MDA were decreased, whereas no significant changes in the activities of SOD, CAT and GSH-Px were observed. The levels of Bax, cleaved caspase-3, and cytochrome c were downregulated, whereas the levels of Bcl-2 and p-Akt/Akt were upregulated. The results of the present study indicated that DhHP-6 may offer therapeutic potential for cerebral ischemia. The neuroprotective effects of DhHP-6 maybe mediated by its anti-oxidative properties, anti-apoptotic activities, or activation of the phosphoinositide 3-kinase/Akt survival pathway.

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Determination of a deuterohemin–peptide conjugate in rat plasma by liquid chromatography–tandem mass spectrometry and application to a preclinical pharmacokinetic study

Cited by 11 publications

References 22 publications

Time-course and intensity-based classifications of oxidative stresses and their potential application in biomedical, comparative and environmental research

Time-course and intensity-based classifications of oxidative stresses and their potential application in biomedical, comparative and environmental research

Enantioselective analysis of lansoprazole in rat plasma by LC–MS/MS: Application to a stereoselective pharmacokinetic study

DhHP-6 attenuates cerebral ischemia-reperfusion injury in rats through the inhibition of apoptosis

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