Scuba diving induces nitric oxide synthesis and the expression of inflammatory and regulatory genes of the immune response in neutrophils

Sureda, Antoni; Batle, Juan M.; Capó, Xavier; Córdova, Alfredo; Tur, Josep A.; Pons, Antoni

doi:10.1152/physiolgenomics.00028.2014

Cited by 36 publications

(30 citation statements)

References 50 publications

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“…This is the first report of MPs expressing nitrotyrosine, a manifestation of protein nitration likely resulting from production of peroxynitrite or some similarly reactive nitrogen species. Increased presence of nitrotyrosine on MPs is consistent with results in a mouse model and also a report of nitrotyrosine elevations in neutrophils obtained from SCUBA divers (21,24). Because dietary ascorbic acid supplementation abrogates elevations of nitrotyrosine-bearing MPs, the results suggest that ascorbic acid acts at sites proximal to pressurerelated production of the reactive species capable of nitration reactions.…”

Section: Discussionsupporting

confidence: 88%

“…There is substantial evidence that oxidative stress occurs with SCUBA diving (3,5,17,(21)(22)(23). We found that prophylactic ingestion of ascorbic acid was effective for inhibiting MP elevations and neutrophil activation due to diving.…”

Section: Discussionmentioning

confidence: 81%

“…SCUBA diving causes upregulation of antioxidant genes and elevations of plasma and intracellular antioxidant enzyme levels (3,5,17,(21)(22)(23). These changes are assumed to occur due to elevations of O 2 partial pressure associated with breathing air at depth.…”

mentioning

confidence: 99%

“…A subset of MPs in mice subjected to high pressure have been shown to contain nitrogen dioxide, which could react with proteins to generate nitrotyrosine (28). Nitrotyrosine is elevated in neutrophils after SCUBA diving and in isolated neutrophils exposed to elevated partial pressures of He, N 2 , or Ar (21,24).…”

mentioning

confidence: 99%

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Ascorbic acid supplementation diminishes microparticle elevations and neutrophil activation following SCUBA diving

Yang

Barak

Dujić

et al. 2015

American Journal of Physiology-Regulatory, Integrative and Comp

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-Predicated on evidence that diving-related microparticle generation is an oxidative stress response, this study investigated the role that oxygen plays in augmenting production of annexin V-positive microparticles associated with open-water SCUBA diving and whether elevations can be abrogated by ascorbic acid. Following a cross-over study design, 14 male subjects ingested placebo and 2-3 wk later ascorbic acid (2 g) daily for 6 days prior to performing either a 47-min dive to 18 m of sea water while breathing air (ϳ222 kPa N2/59 kPa O2) or breathing a mixture of 60% O2/balance N2 from a tight-fitting face mask at atmospheric pressure for 47 min (ϳ40 kPa N2/59 kPa O2). Within 30 min after the 18-m dive in the placebo group, neutrophil activation, and platelet-neutrophil interactions occurred, and the total number of microparticles, as well as subgroups bearing CD66b, CD41, CD31, CD142 proteins or nitrotyrosine, increased approximately twofold. No significant elevations occurred among divers after ingesting ascorbic acid, nor were elevations identified in either group after breathing 60% O2. Ascorbic acid had no significant effect on post-dive intravascular bubble production quantified by transthoracic echocardiography. We conclude that high-pressure nitrogen plays a key role in neutrophil and microparticle-associated changes with diving and that responses can be abrogated by dietary ascorbic acid supplementation.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 81%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Ascorbic acid supplementation diminishes microparticle elevations and neutrophil activation following SCUBA diving

Yang

Barak

Dujić

et al. 2015

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Changes in antioxidant system were also obtained in previous studies after static and dynamic apneas [3,16,17,28]. In addition, scuba diving which is characterized by exercising in hyperoxia increases the mRNA levels of genes related with the inflammatory and immune response in neutrophils [31]. However, in the present study the stimulus is greater as the divers performed around 300 immersions during the 5 days monitored, resulting in a significant antioxidant response.…”

Section: Discussionsupporting

confidence: 71%

Competitive apnea diving sessions induces an adaptative antioxidant response in mononucleated blood cells

et al. 2015

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The aim was evaluating the effects of hypoxia/reoxygenation repetitive episodes during 5 days of apnea diving (3-day training/2-day competition) on peripheral blood mononuclear cells (PBMCs) antioxidant defenses, oxidative damage, and plasma xanthine oxidase activity. Blood samples, from seven professional apnea divers, were taken under basal conditions the previous morning to the first training session (pre-diving basal), 4 h after ending the competition (4 h post-diving) and the following morning (15 h after last dive) in basal conditions (post-diving basal). Glucose levels significantly decreased whereas triglycerides increased at 4 h post-diving, both returning to basal values at post-diving basal. Glutathione reductase and catalase activity significantly increased after 4 h post-diving remaining elevated at post-diving basal. Glutathione peroxidase and superoxide dismutase activities and catalase protein levels progressively increased after diving with significant differences respect to initial values at post-diving basal. No significant differences were observed in circulating PBMCs and oxidative damage markers. Plasma xanthine oxidase activity and nitrite levels, but not the inducible nitric oxide synthetase, significantly increased 4 h post-diving, returning to the basal values after 15 h. In conclusion, chronic and repetitive episodes of diving apnea during five consecutive days increased plasma xanthine oxidase activity and nitric oxide production which could enhance the signalling role of reactive oxygen and nitrogen species for PBMCs antioxidant adaptation against hypoxia/reoxygenation.

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Decompression Modelling and Algorithm

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2022

Engineering and Medicine in Extreme Environments

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Scuba diving induces nitric oxide synthesis and the expression of inflammatory and regulatory genes of the immune response in neutrophils

Abstract: Scuba diving which combines hyperoxia, hyperbaria, and acute exercise induces nitrosative damage with increased nitrotyrosine levels and an inflammatory response in neutrophils.

Cited by 36 publications

References 50 publications

Ascorbic acid supplementation diminishes microparticle elevations and neutrophil activation following SCUBA diving

Ascorbic acid supplementation diminishes microparticle elevations and neutrophil activation following SCUBA diving

Competitive apnea diving sessions induces an adaptative antioxidant response in mononucleated blood cells

Decompression Modelling and Algorithm

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