Reduction of PCR-amplifiable DNA by ethylene oxide treatment of forensic consumables

Neureuther, Katharina; Rohmann, Edyta; Hilken, Manuela; Sonntag, Marie-Luise; Herdt, Silke; Koennecke, Thomas; Jacobs, Roland; Adamski, Michalina; Reisbacher, Stefan; Alfs, Knut; Strain, Peter; Bastisch, Ingo

doi:10.1016/j.fsigen.2014.06.006

Cited by 10 publications

(5 citation statements)

References 16 publications

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“…2). This result is unusual and not demonstrated by prior studies exploring similar effects [17,18,[38][39][40]42]. It is unlikely that inherent template damage or cellular function is responsible for these observations, since the doses applied are beyond those expected to initiate any adaptive DNA repair response [45][46][47].…”

mentioning

confidence: 53%

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Degradation of nuclear and mitochondrial DNA after γ-irradiation and its effect on forensic genotyping

Goodwin

Wotherspoon

Gahan

et al. 2020

Forensic Sci Med Pathol

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confidence: 53%

“…The robustness of STRs to γ-radiation has been demon-378 strated for several STR kits and cell substrates (e.g. blood, 379 saliva) capable of full profiles up to 50 kGy [18,38,39].…”

mentioning

confidence: 99%

Degradation of nuclear and mitochondrial DNA after γ-irradiation and its effect on forensic genotyping

Goodwin

Wotherspoon

Gahan

et al. 2020

Forensic Sci Med Pathol

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“…Although such contamination may occur incidentally, investigation-related contamination can be controlled and should be prevented by effective countermeasures. Among DNA decontamination techniques in forensic science, 4,5,10–12 autoclaving and UV irradiation are prevalent and easy to use in laboratory settings. Our results indicate that the combination of autoclaving and UV irradiation is a more effective optional countermeasure for prevention of amplified STR product contamination in routine cleaning.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have evaluated the effectiveness of sterilisation techniques for DNA decontamination, including ultraviolet (UV), gamma and electron-beam irradiation, ethylene oxide treatment and autoclaving. 4,5,8–13 Autoclaving and UV irradiation are easy-to-use methods, widely applied for eliminating DNA prior to use of instruments and consumables in forensic laboratories. Gefrides et al 4 reported that autoclaving for 120 min at 121°C eliminated PCR-amplifiable DNA from 10 µL of dried saliva.…”

Section: Introductionmentioning

confidence: 99%

Elimination of contaminating amplified short tandem repeat products by autoclaving and ultraviolet irradiation

Ohta

Tanaka

2017

Med Sci Law

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DNA contamination can result in false interpretation of short tandem repeat (STR) DNA typing. Proper decontamination is particularly required in forensic DNA laboratories where probative value of the evidence may be affected. The aim of this study was to establish an effective DNA decontamination procedure for amplified STR products focusing on laboratory-related contamination. We verified the effectiveness of thermally and temporally extended autoclaving and ultraviolet irradiation for the elimination of contaminating amplified STR products. STR amplification products were prepared using a control genomic DNA template and generated using the AmpFℓSTR Identifiler Plus and Yfiler polymerase chain reaction amplification kits. In this study, the contaminants were dried before decontamination treatment, which resembles actual contamination situations. One microlitre of amplified STR products was eliminated by a combination of autoclaving (128°C, 420 min) and UV irradiation (60 J/cm). Our results reveal that the combination treatment represents an effective DNA decontamination procedure and a practicable method in standard level laboratories. Finally, we propose a comprehensive approach for forensic DNA laboratories to implement to minimise contamination issues and guarantee provision of authentic results.

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“…The determination of DNA quantity for degraded samples was subject to error due to the amplicon length used for the polymerase chain reaction (PCR)-based quantitation [4]. As alternative to the quantitation, theĤ proxy proposed by Tvedebrink et al [1] calculated from the peak height has been used as an indicator for the amount of DNA.Ĥ is defined by the sum of all peak heights from a given known profile divided by the sum of the number of heterozygous and homozygous genotypes (homozygous are multiplied by two).…”

Section: Methodsmentioning

confidence: 99%

Drop-out model optimization on degraded DNA samples

Duchamp

Bastisch

Schneider

2015

Forensic Science International: Genetics Supplement Series

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A B S T R A C T Two statistical models have been developed to interpret locus drop-out resulting from degraded DNA samples. These models are based on an experimental dataset of 600 genotyping experiments. The first model presents the probability of locus drop-out against the average peak height with a lower and upper confidence interval. For a given peak height average, the probability of drop-out can be quite wide. This probability decreases rapidly when the profile quality increases. For the second model, we have fitted the probability of locus drop-out against the number of base pairs with a lower and upper confidence interval. The probability of drop-out is increasing along with the number of base pairs. Despite DNA degradation effects, the drop-out probability does not get higher than 0.5.

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Reduction of PCR-amplifiable DNA by ethylene oxide treatment of forensic consumables

Cited by 10 publications

References 16 publications

Degradation of nuclear and mitochondrial DNA after γ-irradiation and its effect on forensic genotyping

Degradation of nuclear and mitochondrial DNA after γ-irradiation and its effect on forensic genotyping

Elimination of contaminating amplified short tandem repeat products by autoclaving and ultraviolet irradiation

Drop-out model optimization on degraded DNA samples

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