Tetralogy of Fallot: aorto-pulmonary collaterals and pulmonary arteries have distinctly different transcriptomes

Ma, Xiaoyuan; Barboza, Laura; Siyahian, Arpi; Reinhartz, Olaf; Maeda, Keisuke; Reddy, V. Mohan; Hanley, Frank L.; Riemer, R. Kirk

doi:10.1038/pr.2014.101

Cited by 8 publications

(4 citation statements)

References 17 publications

(19 reference statements)

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“…That being said, recently a novel approach to evaluate the genetic signatures of pulmonary arteries, AP collaterals, and the aorta was recently undertaken by investigators at Stanford University. 40 Through this study comparing the transcriptomes of these subpopulations of blood vessels, differences in gene expression were identified between the groups. These expression differences spanned several gene ontology categories including developmental processes, anatomical structural development, cell differentiation, and positive regulation of cell migration to name a few.…”

Section: Innovationmentioning

confidence: 99%

An Overview of Pulmonary Atresia and Major Aortopulmonary Collateral Arteries

Presnell

Blankenship

Cheatham

et al. 2015

World J Pediatr Congenit Heart Surg

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Pulmonary atresia with ventricular septal defect and major aortopulmonary collateral arteries (PA/VSD/MAPCAs) is a rare and complex congenital cardiac lesion that has historically carried a poor prognosis. With advancements in surgical management, we have seen an improvement in the outcomes for children affected by this disease. However, this population continues to present challenges due to the complex anatomy and physiology associated with PA/VSD/MAPCA. This summary of material presented during one of the nursing sessions of the 2014 Meeting of the Pediatric Cardiac Intensive Care Society provides an overview for those in cardiac intensive care units who do not have a large experience with this lesion. We will review the anatomy, physiology, surgical approach, postoperative management strategies, and cardiac catheter intervention options for PA/VSD/MAPCAs. We will also discuss recent innovations that may lead to continued improvement in outcomes for this challenging patient population.

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Section: Innovationmentioning

confidence: 99%

An Overview of Pulmonary Atresia and Major Aortopulmonary Collateral Arteries

Presnell

Blankenship

Cheatham

et al. 2015

World J Pediatr Congenit Heart Surg

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“…Real‐time polymerase chain reaction (PCR) was performed as previously reported7 to confirm expression of selected genes identified by microarray analysis. Briefly, total RNA from the 16 RNA pools used for array and additional experiments was reverse‐transcribed (RT) to cDNA using the Superscript II RNase H Reverse Transcriptase kit (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

Critical Role of Coaptive Strain in Aortic Valve Leaflet Homeostasis: Use of a Novel Flow Culture Bioreactor to Explore Heart Valve Mechanobiology

Maeda

Chalajour

et al. 2016

JAHA

Self Cite

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BackgroundAortic valve (AV) disease presents critical situations requiring surgery in over 2% of the US population and is increasingly the reason for cardiac surgery. Throughout the AV cycle, mechanical forces of multiple types and varying intensities are exerted on valve leaflets. The mechanisms whereby forces regulate leaflet homeostasis are incompletely understood. We used a novel flow bioreactor culture to investigate alteration of AV opening or closure on leaflet genes.Methods and ResultsCulture of rat AV was conducted in a flow bioreactor for 7 days at 37°C under conditions approximating the normal stroke volume. Three force condition groups were compared: Cycling (n=8); always open (Open; n=3); or always closed (Closed; n=5). From each culture, AV leaflets were pooled by force condition and RNA expression evaluated using microarrays. Hierarchical clustering of 16 transcriptome data sets from the 3 groups revealed only 2 patterns of gene expression: Cycling and Closed groups clustered together, whereas Open AV were different (P<0.05). Sustained AV opening induced marked changes in expression (202 transcripts >2‐fold; P<0.05), whereas Closed AV exhibited similar expression pattern as Cycling (no transcripts >2‐fold; P<0.05). Comparison with human sclerotic and calcific AV transcriptomes demonstrated high concordance of >40 Open group genes with progression toward disease.ConclusionsFailure of AV to close initiates an extensive response characterized by expression changes common to progression to calcific aortic valve disease. AV coaptation, whether phasic or chronic, preserved phenotypic gene expression. These results demonstrate, for the first time, that coaptation of valve leaflets is a fundamentally important biomechanical cue driving homeostasis.

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“…And when MAPCAs do not narrow, T1/T2 vascular disease develops in the distal vessels due to "PþQ. [45][46][47][48] Developmental origin…”

Section: Histopathological Typesmentioning

confidence: 99%

Pulmonary hypertension’s variegated landscape: a snapshot

Kulik

Austin

2017

Pulm. circ.

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The many types of pulmonary hypertension (PH) are so protean in their biological origin, histological expression, and natural history that it is difficult to create a summary picture of the disease, or to easily compare and contrast characteristics of one type of PH with another. For newcomers to the field, however, such a picture would facilitate a broad understanding of PH. In this paper, we suggest that four characteristics are fundamental to describing the nature of various types of PH, and that taken together they define a number of patterns of PH expression. These characteristics are histopathology, developmental origin, associated clinical conditions, and potential for resolution. The “snapshot” is a way to concisely display the ways that these signal characteristics intersect in select specific types of PH, and is an effort to summarize these patterns in a way that facilitates a “big picture” comprehension of this disease.

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Tetralogy of Fallot: aorto-pulmonary collaterals and pulmonary arteries have distinctly different transcriptomes

Cited by 8 publications

References 17 publications

An Overview of Pulmonary Atresia and Major Aortopulmonary Collateral Arteries

An Overview of Pulmonary Atresia and Major Aortopulmonary Collateral Arteries

Critical Role of Coaptive Strain in Aortic Valve Leaflet Homeostasis: Use of a Novel Flow Culture Bioreactor to Explore Heart Valve Mechanobiology

Pulmonary hypertension’s variegated landscape: a snapshot

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