25-hydroxycholesterol interacts differently with lipids of the inner and outer membrane leaflet – The Langmuir monolayer study complemented with theoretical calculations

Wnętrzak, Anita; Chachaj‐Brekiesz, Anna; Kuś, Karolina; Filiczkowska, Anna; Lipiec, Ewelina; Kobierski, Jan; Petelska, Aneta D.; Dynarowicz-Łątka, Patrycja

doi:10.1016/j.jsbmb.2021.105909

Cited by 10 publications

(3 citation statements)

References 66 publications

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“…For systems composed of 25-OH mixed with membrane phospholipids characteristic for the outer (PC) and cytosolic (PE) leaflet it was shown that the interaction strength is primarily determined by the type of phospholipid polar head [ 121 ]. Namely, strong, attractive interactions leading to the formation of surface complexes were observed for 25-OH/PC, while weak or repulsive interactions occurred in 25-OH/PE systems.…”

Section: Interactions In Multi-component Biomimetic Systemsmentioning

confidence: 99%

“…In contrast, for unsaturated PC (25-OH/DOPC), only one specific orientation of oxysterol was shown (anchoring with C(3)-OH in water). All these indicate that the transport of 25-OH between the membrane leaflets can proceed without changing the orientation of oxysterol molecule, which is thermodynamically favourable [ 121 ]. This explains significant differences in the transmembrane transport rate of 25-OH and other chain-oxidized oxysterols compared with their ring-oxidized analogues or cholesterol [ 122 , 123 ].…”

Section: Interactions In Multi-component Biomimetic Systemsmentioning

confidence: 99%

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Advantages of the classical thermodynamic analysis of single—and multi-component Langmuir monolayers from molecules of biomedical importance—theory and applications

Dynarowicz-Latka,

Wnętrzak,

Chachaj-Brekiesz

2024

J. R. Soc. Interface.

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The Langmuir monolayer technique has been successfully used for decades to model biological membranes and processes occurring at their interfaces. Classically, this method involves surface pressure measurements to study interactions within membrane components as well as between external bioactive molecules (e.g. drugs) and the membrane. In recent years, surface-sensitive techniques were developed to investigate monolayers in situ ; however, the obtained results are in many cases insufficient for a full characterization of biomolecule-membrane interactions. As result, description of systems using parameters such as mixing or excess thermodynamic functions is still relevant, valuable and irreplaceable in biophysical research. This review article summarizes the theory of thermodynamics of single- and multi-component Langmuir monolayers. In addition, recent applications of this approach to characterize surface behaviour and interactions (e.g. orientation of bipolar molecules, drug-membrane affinity, lateral membrane heterogeneity) are presented.

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Section: Interactions In Multi-component Biomimetic Systemsmentioning

confidence: 99%

Section: Interactions In Multi-component Biomimetic Systemsmentioning

confidence: 99%

Advantages of the classical thermodynamic analysis of single—and multi-component Langmuir monolayers from molecules of biomedical importance—theory and applications

Dynarowicz-Latka,

Wnętrzak,

Chachaj-Brekiesz

2024

J. R. Soc. Interface.

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“…Cholesterol is a regulator of signaling molecules and functional proteins, and it also happens to be the binding site of AmB affinity to cell membranes. Dipalmitoyl phosphatidylcholine (DPPC) is a kind of saturated phospholipid that is often present in the outer cell membrane leaflet ( Wnętrzak et al, 2021 ). Due to its clear phase transitions and well-defined mechanical and thermodynamic properties, DPPC has been widely used in Langmuir monolayers as a membrane model ( Ruiz et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of high sodium ion level on the interaction of AmB with a cholesterol-rich phospholipid monolayer

Wang,

Qiang,

et al. 2024

Front. Mol. Biosci.

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Invasive fungal infections are a primary reason for high mortality in immunocompromised people, especially in critically ill patients, such as intensive care unit (ICU) patients, advanced cancer patients, or severe burn patients. Hypernatremia also can increase mortality in severely ill patients. Amphotericin B (AmB) is the gold standard for treating infections, but in severely ill patients, AmB can cause hematotoxicity when administered intravenously due to its interaction with cholesterol on red blood cell membranes. This results in limited doses of AmB and affects the treatment of infections. The proportion of cholesterol molecules in membrane lipids in red blood cells is as high as 50 mol%, and the sodium ions can influence the interaction between AmB and lipids on the membrane. Therefore, in the complex clinical situation of a severely ill patient with a fungal infection and hypernatremia, the interaction between amphotericin B and the red blood cell membranes is worth studying in depth. In this work, the interaction between AmB and the dipalmitoyl phosphatidylcholine (DPPC)/cholesterol mixed monolayer in the presence of high sodium ion levels was studied when the proportion of cholesterol was 50%. The results show that the effect of AmB on reducing the monolayer’s area at a high level of sodium ions is slightly stronger at 30 mN/m. The effect of AmB on reducing the elastic modulus of the DPPC/Chol monolayer is significantly weakened by a high sodium ion level, compared with the level of sodium ions at normal physiological concentration. The higher the sodium ion concentration, the weaker the intermolecular force of the DPPC/Chol/AmB mixed monolayers. The scanning electron microscope (SEM) and atomic force microscopy (AFM) observations suggest that at a high sodium ion level, the presence of AmB significantly reduces the surface roughness of the DPPC/Chol monolayer. AmB may bind to cholesterol molecules, and it isolates cholesterol from the monolayer, resulting in a reduced height of the cholesterol-rich monolayer and an increasingly dispersed monolayer region. The results are beneficial to understanding the mechanism of impact of a high sodium ion level on the relationship between AmB and red blood cell membranes rich in cholesterol and are valuable for understanding the hemolytic toxicity of AmB to red blood cells at a high sodium ion level.

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The Structure of Oxysterols Determines Their Behavior at Phase Boundaries: Implications for Model Membranes and Structure–Activity Relationships

Wnętrzak,

Chachaj-Brekiesz,

Kobierski

et al. 2023

Advances in Experimental Medicine and Biology

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25-hydroxycholesterol interacts differently with lipids of the inner and outer membrane leaflet – The Langmuir monolayer study complemented with theoretical calculations

Cited by 10 publications

References 66 publications

Advantages of the classical thermodynamic analysis of single—and multi-component Langmuir monolayers from molecules of biomedical importance—theory and applications

Advantages of the classical thermodynamic analysis of single—and multi-component Langmuir monolayers from molecules of biomedical importance—theory and applications

Effect of high sodium ion level on the interaction of AmB with a cholesterol-rich phospholipid monolayer

The Structure of Oxysterols Determines Their Behavior at Phase Boundaries: Implications for Model Membranes and Structure–Activity Relationships

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