Enhanced anaerobic biodegradation of OCDD-contaminated soils by Pseudomonas mendocina NSYSU: Microcosm, pilot-scale, and gene studies

Tu, Y.T.; Liu, J.K.; Lin, Wei-Chih; Jing, Lin; Kao, C. L.

doi:10.1016/j.jhazmat.2014.06.014

Cited by 22 publications

(13 citation statements)

References 49 publications

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“…All bottles were sealed with Teflon-lined rubber septa. Procedures of mineral solution preparation is described in Tu et al (2014). Table 1 shows the components of six groups of aerobic microcosms.…”

Section: Methodsmentioning

confidence: 99%

“…Microcosms were incubated at room temperature (20 8C) in dark. The soil extraction procedures and OCDD analytical methods were described in Tu et al (2014). The OCDD degradation efficiency was determined as a percentage of the initial OCDD concentration.…”

Section: Methodsmentioning

confidence: 99%

“…Although many microbes could biodegrade low-chlorinated dioxins, relatively less knowledge is available about the biodegradation of higher-chlorinated dioxins aerobically (Jeon et al, 2016;Urbaniak, 2013;Tu et al, 2014). The goals of this microcosm study were to assess the (1) effectiveness of enhancing OCDD biodegradation under aerobic conditions by P. mendocina NSYSU with the addition of lecithin, (2) effectiveness of inducing OCDD ring-cleavage genes by PCP and OCDD addition using the gene inducement method, and (3) genes responsible for aerobic OCDD biodegradation.…”

Section: Introductionmentioning

confidence: 99%

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Aerobic Biodegradation of OCDD by P. Mendocina NSYSU: Effectiveness and Gene Inducement Studies

Jing

Lin

Liu

et al. 2017

Water Environment Research

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The goals of this study were to assess the effectiveness of (1) enhancing octachlorinated dibenzo-p-dioxin (OCDD) biodegradation under aerobic conditions by Pseudomonas mendocina NSYSU (P. Mendocina NSYSU) with the addition of lecithin, and (2) inducing OCDD ring-cleavage genes by pentachlorophenol (PCP) and OCDD addition. P. Mendocina NSYSU could biodegrade OCDD via aerobic cometabolism and lecithin was used as a primary substrate. Approximately 74 and 67% of OCDD biodegradation was observed after 60 days of incubation with lecithin and glucose supplement, respectively. Lecithin was also used as the solubilization additive resulting in OCDD solubilization and enhanced bioavailability of OCDD to P. Mendocina NSYSU. Two intradiol and extradiol ring-cleavage dioxygenase genes (Pmen_0474 and Pmen_2526) were identified from gene analyses. Gene concentration was significantly enhanced after the inducement by PCP and OCDD. Higher gene inducement efficiency was obtained using PCP as the inducer, and Pmen_2526 played a more important role in OCDD biodegradation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aerobic Biodegradation of OCDD by P. Mendocina NSYSU: Effectiveness and Gene Inducement Studies

Jing

Lin

Liu

et al. 2017

Water Environment Research

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“…Each batch bottle contained 1 mL of lecithin (or 1 mL of mineral medium solution), 20 g of site soils, 5 mL of P. mendocina NSYSU solution as inocula (or 5 mL of mineral solution), and 35 mL of nutrient mineral medium (autoclaved before use) in a 70-mL serum bottle, which was sealed with Teflon-lined rubber septa. The components of nutrient mineral solution (no carbon component) were described in Tu et al (2014). The procedures of anaerobic microcosm preparation were described in Tu et al (2014).…”

Section: Batch Studymentioning

confidence: 99%

“…The components of nutrient mineral solution (no carbon component) were described in Tu et al (2014). The procedures of anaerobic microcosm preparation were described in Tu et al (2014). Table 1 lists the constituents of different microcosms.…”

Section: Batch Studymentioning

confidence: 99%

Bioremediation of Ocdf-Contaminated Soils by Novel Bacterial Strain

Jing

Chen²,

Sung

et al. 2017

Appl Ecol Env Res

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Abstract. In this study, effectiveness of using Pseudomonas mendocina NSYSU (P. mendocina NSYSU) on the bioremediation of octachlorinated dibenzofuran (OCDF)-polluted soils was evaluated through microcosm experiments and gene analys is under anaerobic conditions. The major tasks were to investigate the (1) feasibility of enhancing anaerobic OCDF dechlorination by P. mendocina NSYSU and lecithin, and (2) functional genes for the anaerobic biodegradation of OCDF. Results show that P. mendocina NSYSU was able to degrade OCDF through the anaerobic dechlorinating mechanisms. Results show that lecithin was used as the carbon source and OCDF desorption additive during the dechlorination of OCDF. Lecithin supplement could enhance the biodegradation rate of OCDF. Up to 68 and 61% of OCDF was removed after a 64-day operation with lecithin and nutrient broth supplement, respectively. Results indicate that primary substrate supplement is required for the enhancement of reductive dechlorination of OCDF. Five functional genes encoding the hydrolase in P. mendocina NSYSU were identified. The detected specific genes played important roles in OCDF dechlorination. Results reveal that a bioremediation system using P. mendocina NSYSU as the inocula would be a cost-effective and acceptable remedial system to remediate furan-polluted soils.

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Alteration of the Catalytic Reaction Trajectory of a Vicinal Oxygen Chelate Enzyme by Directed Evolution

et al. 2022

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The vicinal oxygen chelate (VOC) metalloenzyme superfamily catalyzes a highly diverse set of reactions with the mechanism characterized by the bidentate coordination of vicinal oxygen atoms to metal ion centers, but there remains a lack of a platform to steer the reaction trajectories, especially for o-quinone metabolizing pathways. Herein, we present the directedevolution-enabled bifunctional turnover of ChaP, which is a homotetramer and represents an unprecedented VOC enzyme class. Unlike the ChaP catalysis of extradiol-like o-quinone cleavage and concomitant αketo acid decarboxylation, a group of ChaP variants (CVs) catalyze intradiol-like o-quinone deconstruction and CO 2 liberation from the resulting o-hydroxybenzoic acid scaffolds with high regioselectivity. Enzyme crystal structures, labeling experiments and computational simulations corroborated that the D49L mutation allows the metal ion to change its coordination with the tyrosine phenoxy atoms in different monomers, thereby altering the reaction trajectory with the regiospecificity further improved by the follow-up replacement of the Y92 residue with any of alanine, glycine, threonine, and serine. The study highlights the unpredicted catalytic versatility and enzymatic plasticity of VOC enzymes with biotechnological significance.

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Enhanced anaerobic biodegradation of OCDD-contaminated soils by Pseudomonas mendocina NSYSU: Microcosm, pilot-scale, and gene studies

Cited by 22 publications

References 49 publications

Aerobic Biodegradation of OCDD by P. Mendocina NSYSU: Effectiveness and Gene Inducement Studies

Aerobic Biodegradation of OCDD by P. Mendocina NSYSU: Effectiveness and Gene Inducement Studies

Bioremediation of Ocdf-Contaminated Soils by Novel Bacterial Strain

Alteration of the Catalytic Reaction Trajectory of a Vicinal Oxygen Chelate Enzyme by Directed Evolution

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