Impact of Third-Generation-Cephalosporin Administration in Hatcheries on Fecal Escherichia coli Antimicrobial Resistance in Broilers and Layers

Baron, Sandrine; Jouy, Éric; Larvor, Emeline; Eono, F.; Bougeard, Stéphanie; Kempf, Isabelle

doi:10.1128/aac.03106-14

Cited by 62 publications

(66 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite the high prevalence at day 7, CMY-2-E. coli was not able to persist in the chickens. Other studies in poultry have also shown a decreasing prevalence of antibiotic resistant bacteria, with and without the use of antibiotics (Diarra et al, 2007;Baron et al, 2014;Huijbers et al, 2015). However, most of these studies report limited reduction.…”

Section: Resultsmentioning

confidence: 84%

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dame-Korevaar

Fischer

Stegeman

et al. 2017

Veterinary Microbiology

View full text Add to dashboard Cite

A B S T R A C TExtended-spectrum β-lactamase and plasmid mediated AmpC β-lactamase (ESBL/pAmpC) producing bacteria are resistant to Extended Spectrum Cephalosporins (ESC), and are present in all levels of the broiler production chain. We determined the prevalence, concentration, and persistence of ESBL/pAmpC-Escherichia coli in a broiler parent flock during the rearing and laying period. One-day old chickens were housed in four separate pens. Until week 33 no antibiotics or coccidiostatics were used. During rearing 57 chickens in each pen (n = 228), and in the laying period two groups of 33 chickens were individually sampled (n = 66). Environmental samples were taken from week 16 onwards. ESBL/pAmpC-E. coli presence was determined by selective culturing. In the samples of week 16-19 the concentration of ESBL/pAmpC-E. coli was determined. All ESC-resistant isolates found were positive for pAmpC gene bla CMY-2 located on IncA/C plasmids, in several E. coli MLST types. CMY-2-E. coli prevalence decreased from 91% (95%CI 86-94%) at day 7 (week 1) to 0% (95%CI 0-5%) in week 21. However, CMY-2-E. coli remained present in the environmental samples during the whole study. CMY-2-E. coli concentration varied between detection limit (< 10^3) and 2·10^4 cfu/g faeces. The sharp reduction of CMY-2-E. coli in this broiler parent flock in absence of antibiotics suggests a selective disadvantage of bla CMY-2 on IncA/C plasmids on animal level. The underlying mechanism should be studied further as this may provide new insights on how to reduce ESBL/pAmpC prevalence and transmission in the broiler production chain.

show abstract

Section: Resultsmentioning

confidence: 84%

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dame-Korevaar

Fischer

Stegeman

et al. 2017

Veterinary Microbiology

View full text Add to dashboard Cite

show abstract

“…Unfortunately, as expected, it has been demonstrated that the use of antimicrobial agents in husbandry is directly related to the incidence of resistant bacteria in FPA (Baron et al . ; Chantziaras et al . ).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance in Escherichia coli in husbandry animals: the African perspective

Alonso

Zarazaga

Sallem

et al. 2017

Letters in Applied Microbiology

133

106

View full text Add to dashboard Cite

In the last few years, different surveillances have been published in Africa, especially in northern countries, regarding antimicrobial resistance among husbandry animals. Information is still scarce, but the available data show a worrying picture. Although the highest resistance rates have been described against tetracycline, penicillins and sulphonamides, prevalence of plasmidmediated quinolone resistance genes and extended spectrum b-lactamase (ESBL) are being increasingly reported. Among ESBLs, the CTX-M-1 group was dominant in most African surveys. Within this group, CTX-M-15 was the main variant both in animals and humans, except in Tunisia where CTX-M-1 was more frequently detected among Escherichia coli from poultry. Certain bla CTX-M-15 -harbouring clones (ST131/B2 or ST405/D) are mainly identified in humans, but they have also been reported in livestock species from Tanzania, Nigeria or Tunisia. Moreover, several reports suggest an inter-host circulation of specific plasmids (e.g. bla CTX-M-1 -carrying IncI1/ST3 in Tunisia, IncY-and Incuntypeable replicons co-harbouring qnrS1 and bla CTX-M-15 in Tanzania and the worldwide distributed bla CTX-M-15 -carrying IncF-type plasmids). International trade of poultry meat seems to have contributed to the spread of other ESBL variants, such as CTX-M-14, and clones. Furthermore, first descriptions of OXA-48-and OXA-181-producing E. coli have been recently documented in cattle from Egypt, and the emergent plasmid-mediated colistin resistance mcr-1 gene has been also identified in chickens from Algeria, Tunisia and South Africa. These data reflect the urgent need of a larger regulation in the use of veterinary drugs and the implementation of surveillance programmes in order to decelerate the advance of antimicrobial resistance in this continent.

show abstract

“…Investigations of ESBL and AmpC‐mediated β‐lactam and cephalosporin resistance among poultry pointed to the use of cephalosporins in hatcheries (administered in ovo or at hatching) as a likely driver for colonization of birds by E. coli (and, in one study, Salmonella ) that were cephalosporin resistant (Dutil et al., ; Persoons et al., ), with one study showing hatchery use to be highly significant in a multivariable regression model for the flock‐level prevalence of cephalosporin resistant E. coli (Baron et al., ). More broadly, recent rapid expansion of ESBL‐type resistance among nonclonal isolates of E. coli from livestock strongly suggests horizontal transmission as the dominant mode of resistance acquisition (Scientific Advisory Group on Antimicrobials of the Committee for Medicinal Products for Veterinary Use, ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Davies

Wales

2019

Comp Rev Food Sci Food Safe

120

View full text Add to dashboard Cite

Resistance to therapeutic antimicrobial agents is recognized as a growing problem for both human and veterinary medicine, and the need to address the issue in both of these linked domains is a current priority in public policy. Efforts to limit antimicrobial resistance (AMR) on farms have so far focused on control of the supply and use of antimicrobial drugs, plus husbandry measures to reduce infectious disease. In the United Kingdom and some other countries, substantial progress has been made recently against targets on agricultural antimicrobial drug use. However, evidence suggests that resistant pathogenic and commensal bacteria can persist and spread within and between premises despite declining or zero antimicrobial drug use. Reasons for this are likely complex and varied but may include: bacterial adaptations to ameliorate fitness costs associated with maintenance and replication of resistance genes and associated proteins, horizontal transmission of genetic resistance determinants between bacteria, physical transfer of bacteria via movement (of animals, workers, and equipment), ineffective cleaning and disinfection, and co‐selection of resistance to certain drugs by use of other antimicrobials, heavy metals, or biocides. Areas of particular concern for public health include extended‐spectrum cephalosporinases and fluoroquinolone resistance among Enterobacteriaceae, livestock‐associated methicillin‐resistant Staphylococcus aureus, and the emergence of transmissible colistin resistance. Aspects of biosecurity have repeatedly been identified as risk factors for the presence of AMR on farm premises, but there are large gaps in our understanding of the most important risk factors and the most effective interventions. The present review aims to summarize the present state of knowledge in this area, from a European perspective.

show abstract

Impact of Third-Generation-Cephalosporin Administration in Hatcheries on Fecal Escherichia coli Antimicrobial Resistance in Broilers and Layers

Cited by 62 publications

References 27 publications

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Antibiotic resistance in Escherichia coli in husbandry animals: the African perspective

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Contact Info

Product

Resources

About