Oestrogen and progesterone receptor expression in subtypes of canine mammary tumours in intact and ovariectomised dogs

Mainenti, Marta; Rasotto, Roberta; Carnier, Paolo; Zappulli, Valentina

doi:10.1016/j.tvjl.2014.06.003

Cited by 22 publications

(24 citation statements)

References 21 publications

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“…Thus, there might be an association between low ESR1, PGR and PRLR expression and the associated prognosis of the tumor. Similarly, a previous study which measured ESR1/PGR in specific histological subtypes in 113 CMT by IHC found an association between low hormone receptor labeling and aggressive neoplastic behavior of the histological subtypes [ 21 ]. Similar results have been reported in human breast cancer for both ESR1 and PGR expression [ 27 – 28 ].…”

Section: Discussionmentioning

confidence: 68%

“…The aim of the present study was to investigate a multiplex bDNA assay for analyzing hormone receptor gene expression in canine mammary tissue comparing fresh frozen and FFPE samples. Until now, most studies evaluating hormone receptors in CMT have used IHC [ 21 – 24 ] referring to the method of choice in human breast cancer. However, results of IHC can be diverse depending on the sample fixation type and time, antibody clones and assay interpretation utilized in different studies [ 2 ].…”

Section: Discussionmentioning

confidence: 99%

“…PR levels were only lower in malignant tumors of the FFPE samples, but not in the fresh frozen specimens. This might be due to small sample numbers (fresh frozen n = 16, FFPE n = 13) considering that in a recent study, ESR1/PGR in malignant mammary tumors was found to be significantly reduced in 30 neutered females compared to 49 intact females with malignant mammary tumors [ 21 ]. Thus, the influence of castration time on mammary tumor development and hormone receptor expression needs to be further evaluated.…”

Section: Discussionmentioning

confidence: 99%

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Hormone Receptor Expression Analyses in Neoplastic and Non-Neoplastic Canine Mammary Tissue by a Bead Based Multiplex Branched DNA Assay: A Gene Expression Study in Fresh Frozen and Formalin-Fixed, Paraffin-Embedded Samples

et al. 2016

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Immunohistochemistry (IHC) is currently considered the method of choice for steroid hormone receptor status evaluation in human breast cancer and, therefore, it is commonly utilized for assessing canine mammary tumors. In case of low hormone receptor expression, IHC is limited and thus is complemented by molecular analyses. In the present study, a multiplex bDNA assay was evaluated as a method for hormone receptor gene expression detection in canine mammary tissues. Estrogen receptor (ESR1), progesterone receptor (PGR), prolactin receptor (PRLR) and growth hormone receptor (GHR) gene expressions were evaluated in neoplastic and non-neoplastic canine mammary tissues. A set of 119 fresh frozen and 180 formalin-fixed, paraffin-embedded (FFPE) was comparatively analyzed and used for assay evaluation. Furthermore, a possible association between the hormone receptor expression in different histological subtypes of canine malignant mammary tumors and the castration status, breed and invasive growth of the tumor were analyzed. The multiplex bDNA assay proved to be more sensitive for fresh frozen specimens. Hormone receptor expression found was significantly decreased in malignant mammary tumors in comparison to non-neoplastic tissue and benign mammary tumors. Among the histological subtypes the lowest gene expression levels of ESR1, PGR and PRLR were found in solid, anaplastic and ductal carcinomas. In summary, the evaluation showed that the measurement of hormone receptors with the multiplex bDNA assay represents a practicable method for obtaining detailed quantitative information about gene expression in canine mammary tissue for future studies. Still, comparison with IHC or quantitative real-time PCR is needed for further validation of the present method.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Hormone Receptor Expression Analyses in Neoplastic and Non-Neoplastic Canine Mammary Tissue by a Bead Based Multiplex Branched DNA Assay: A Gene Expression Study in Fresh Frozen and Formalin-Fixed, Paraffin-Embedded Samples

et al. 2016

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“…In veterinary medicine, some studies have shown that the expression of one or both receptors was more frequent in benign tumors, and in general, its expression was related to a more favorable prognosis [ 87 , 89 , 91 ]. Though, in other studies, increased levels of estrogen, progesterone and prolactin were found in cases of malignant neoplasms [ 81 , 95 ].…”

Section: Hormone Receptorsmentioning

confidence: 99%

Current biomarkers of canine mammary tumors

et al. 2018

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Mammary tumors are the second most common neoplasia in dogs. Due to the high similarity of canine mammary tumors (CMT) to human breast cancers (HBC), human biomarkers of HBC are also detectable in cases of CMT. The evaluation of biomarkers enables clinical diagnoses, treatment options and prognosis for bitches suffering from this disease. The aim of this article is to give a short summary of the biomarkers of CMT based on current literature. Very promising biomarkers are miRNAs, cancer stem cells, and circulating tumor cells, as well as mutations of the breast cancer 1 gene (BRCA1) and breast cancer 2 gene (BRCA2). Until now, the most studied and reliable biomarkers of CMT have remained antigen Ki-67 (Ki-67), endothelial growth factor receptor, human epidermal growth factor receptor 2 (HER-2), estrogen receptor, progesterone receptor and cyclooxygenase 1 (COX-2), which can be detected in both serum and tissue samples using different molecular methods. However, carcinoembryonic antigen and cancer antigen 15-3 (CA 15-3), while poorly studied, seem to be good biomarkers, especially for the early detection and prognosis of CMT. We will also mention the following: proliferative cell nuclear antigen, tumor protein p53 (p53), E-cadherin, vascular endothelial growth factor, microRNAs, cancer stem cells and circulating tumor cells, which can also be useful biomarkers. Although many studies have been conducted so far, the estimation of biomarkers in cases of CMT is still not a common practice, and more detailed research should be done.

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“…Die Rezeptorexpression für Sexualsteroidhormone unterscheidet sich zudem zwischen benignen und malignen Neoplasien der Mamma und normalem Mammagewebe (23,37). Die geringere Expression der Steroidrezeptoren in malignen Tumoren weist auf einen Verlust der Steroidabhängigkeit während der Entstehung bösartiger Tumoren hin (66,67,72) und erklärt, warum ein protektiver Effekt bei einer Kastration im fortgeschrittenen Alter vor allem hinsichtlich der Entstehung benigner Neoplasien besteht (58,84). Liegen bereits maligne MT vor, scheint die gleichzeitig mit der MT-Entfernung durchgeführte Kastration bei der Mehrzahl der Hunde den Krankheitsverlauf nicht zu beeinflussen (111).…”

Section: Mammatumorenunclassified

Kastration der Hündin – neue und alte Erkenntnisse zu Vor- und Nachteilen

Arlt¹,

Wehrend²,

Reichler³

2017

Tierarztl Prax Ausg K

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ZusammenfassungDie Beratung von Hundehaltern bezüglich der Vorteile und Nachteile sowie des optimalen Zeitpunktes der Kastration einer Hündin ist komplex und sollte sich auf verlässliche Daten aus der Wissenschaft stüt-zen sowie individuelle Belange berücksichtigen. Bei genauerer Betrachtung der aktuellen Publikationen zu diesem Thema fällt auf, dass einige lange für robust gehaltene Informationen etwas angepasst oder aktualisiert werden müssen. Ein offensichtlicher Vorteil der elektiven Kastration ist, dass Erkrankungen der Ovarien und sexualsteroidabhängige Erkrankungen wie Metropathien nicht auftreten. Weiterhin ist ein gewisser protektiver Effekt einer frühzeitigen Kastration auf die Entstehung von Mammatumoren anzunehmen, wenngleich dafür derzeit eine schwache wissenschaftliche Evidenz besteht und der Effekt vermutlich geringer ausfällt, als in einigen älteren Publikationen angegeben wurde. Die Kastration hat jedoch auch Nachteile. Als häufigste negative Folge wird seit Jahrzehnten die Harninkontinenz beschrieben. Aussagen zur Inzidenz sowie zu weiteren Einflussfaktoren, wie Kastrationszeitpunkt in Bezug zur Pubertät, sind jedoch uneinheitlich. Aktuell werden vor allem das gehäufte Auftreten verschiedener Tumorerkrankungen, die nicht direkt mit dem Reproduktionstrakt in Verbindung stehen, sowie ein höheres Risiko für Erkrankungen des Bewegungsapparats bei kastrierten Tieren diskutiert. Weitere Einflussfaktoren wie der Ernährungszustand des Hundes, Alter und/oder Haltung wurden jedoch häufig nicht erfasst. Die Datenlage zur Auswirkung der Kastration auf den Stoffwechsel und das Immunsystem der Hündin ist derzeit ungenügend. Wünscht der Tierbesitzer eine Kastration, bedarf es in jedem Fall einer eingehenden Beratung, die individuelle, rasse-und haltungsbezogene Aspekte einschließt. Derzeit ist eine Kastration am ehesten zwischen der ersten und zweiten Läufigkeit zu empfehlen, da ein gewisser protektiver Effekt auf die Entstehung von Mammatumoren anzunehmen ist und das Risiko unerwünschter Nebenwirkungen nach heutigem Wissensstand moderat ausfällt. KeywordsNeutering of female dogs, ovariohysterectomy, guidance in decision making, side effects SummaryAdvice for dog owners regarding the Pros and Cons and optimal time for neutering a female dog is complex and should be based on recent and valid research data as well as individual aspects. After taking a closer look at the literature, it becomes clear that some putative robust information has to be revised or updated. An obvious advantage of neutering a female dog is that ovarian diseases and sexual steroiddependent diseases, including metropathy, no longer occur. In addition, it is likely that an early neutering reduces the risk to a certain extent of mammary neoplasia, even if the scientific basis for this observation is weak. The effect might be less than some earlier publications suggest. Disadvantages of neutering female dogs include urinary incontinence, which was postulated decades earlier. However, reported incidences and findings regarding factors that in...

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Oestrogen and progesterone receptor expression in subtypes of canine mammary tumours in intact and ovariectomised dogs

Cited by 22 publications

References 21 publications

Hormone Receptor Expression Analyses in Neoplastic and Non-Neoplastic Canine Mammary Tissue by a Bead Based Multiplex Branched DNA Assay: A Gene Expression Study in Fresh Frozen and Formalin-Fixed, Paraffin-Embedded Samples

Hormone Receptor Expression Analyses in Neoplastic and Non-Neoplastic Canine Mammary Tissue by a Bead Based Multiplex Branched DNA Assay: A Gene Expression Study in Fresh Frozen and Formalin-Fixed, Paraffin-Embedded Samples

Current biomarkers of canine mammary tumors

Kastration der Hündin – neue und alte Erkenntnisse zu Vor- und Nachteilen

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