Effects of duodenal switch alone or in combination with sleeve gastrectomy on body weight and lipid metabolism in rats

Gudbrandsen, Oddrun Anita; Kodama, Yosuke; Mjøs, Svein A.; Zhao, Chun‐Mei; Johannesen, Helene; Brattbakk, Hans-Richard; Haugen, Christine E.; Kulseng, Bård; Mellgren, Gunnar; Chen, Duan

doi:10.1038/nutd.2014.22

Cited by 3 publications

(2 citation statements)

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“…After evaporating the solvent the samples were derivatized to fatty acid methyl esters (FAME) by direct esterification in methanolic HCl at 90 °C for 2 h under nitrogen atmosphere (Meier et al 2006 ). FAMEs were extracted and analyzed by gas chromatography as described in Gudbrandsen et al ( 2014 ). The samples were run in a randomized sequence and the FAME reference mixture GLC-461 (Nu-Chek Prep, Elysian, MN, USA) was analyzed as every 10th sample.…”

Section: Methodsmentioning

confidence: 99%

Predictive associations between serum fatty acids and lipoproteins in healthy non-obese Norwegians: implications for cardiovascular health

et al. 2015

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A battery of methods for multivariate data analysis has been used to assess the associations between concentrations of fatty acids (FAs) and lipoprotein subclasses and particle size in serum for a normolipidemic population of ethnic Norwegians living in the rural Fjord region. Significant gender differences were found in the lipoprotein and FA patterns. Predictive FA patterns were revealed for lipoprotein features of importance for cardiovascular (CV) health. Thus, the subclasses of atherogenic small and very small low density lipoprotein (LDL) particles and the same subclasses of high density lipoprotein (HDL) particles were associated with a pattern of saturated FAs and mono-unsaturated C16-C18 FAs. Eicosapentaenoic acid (EPA) and the ratio of EPA to arachidonic acid (AA) had strongest associations to features that promotes CV health: (i) large average size of HDL and LDL particles, and, (ii) small average size of very low density lipoprotein (VLDL) particles. Total concentration of HDL in both genders correlated to EPA, but docosahexaenoic acid (DHA) correlated just as strongly for women. For men, docosapentaenoic acid (DPA) showed stronger association to HDL concentration than EPA. For both genders, concentration of large LDL particles showed associations to levels of EPA, but stronger to DHA and DPA. High values of EPA/AA seem to be the strongest single biomarker for good CV health in both men and women.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-015-0886-4) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Predictive associations between serum fatty acids and lipoproteins in healthy non-obese Norwegians: implications for cardiovascular health

et al. 2015

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“…They used the Bligh and Dyer method with the addition of heneicosanoic acid as an internal standard. Then, the extracts were methylated in anhydrous MeOH containing 2.5 M HCl (100 °С; 2 h) and extracted twice with isooctane, and methyl esters were quantified using GC with an FID [ 187 ].…”

Section: Preparation Of Different Sample Typesmentioning

confidence: 99%

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

et al. 2020

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Obesity is associated with alterations in the composition and amounts of lipids. Lipids have over 1.7 million representatives. Most lipid groups differ in composition, properties and chemical structure. These small molecules control various metabolic pathways, determine the metabolism of other compounds and are substrates for the syntheses of different derivatives. Recently, lipidomics has become an important branch of medical/clinical sciences similar to proteomics and genomics. Due to the much higher lipid accumulation in obese patients and many alterations in the compositions of various groups of lipids, the methods used for sample preparations for lipidomic studies of samples from obese subjects sometimes have to be modified. Appropriate sample preparation methods allow for the identification of a wide range of analytes by advanced analytical methods, including mass spectrometry. This is especially the case in studies with obese subjects, as the amounts of some lipids are much higher, others are present in trace amounts, and obese subjects have some specific alterations of the lipid profile. As a result, it is best to use a method previously tested on samples from obese subjects. However, most of these methods can be also used in healthy, nonobese subjects or patients with other dyslipidemias. This review is an overview of sample preparation methods for analysis as one of the major critical steps in the overall analytical procedure.

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Active Smarter Kids (ASK): Rationale and design of a cluster-randomized controlled trial investigating the effects of daily physical activity on children’s academic performance and risk factors for non-communicable diseases

Resaland¹,

Moe²,

Aadland³

et al. 2015

BMC Public Health

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BackgroundEvidence is emerging from school-based studies that physical activity might favorably affect children’s academic performance. However, there is a need for high-quality studies to support this. Therefore, the main objective of the Active Smarter Kids (ASK) study is to investigate the effect of daily physical activity on children’s academic performance. Because of the complexity of the relation between physical activity and academic performance it is important to identify mediating and moderating variables such as cognitive function, fitness, adiposity, motor skills and quality of life (QoL). Further, there are global concerns regarding the high prevalence of lifestyle-related non-communicable diseases (NCDs). The best means to address this challenge could be through primary prevention. Physical activity is known to play a key role in preventing a host of NCDs. Therefore, we investigated as a secondary objective the effect of the intervention on risk factors related to NCDs. The purpose of this paper is to describe the design of the ASK study, the ASK intervention as well as the scope and details of the methods we adopted to evaluate the effect of the ASK intervention on 5thgrade children.Methods & designThe ASK study is a cluster randomized controlled trial that includes 1145 fifth graders (aged 10 years) from 57 schools (28 intervention schools; 29 control schools) in Sogn and Fjordane County, Norway. This represents 95.3 % of total possible recruitment. Children in all 57 participating schools took part in a curriculum-prescribed physical activity intervention (90 min/week of physical education (PE) and 45 min/week physical activity, in total; 135 min/week). In addition, children from intervention schools also participated in the ASK intervention model (165 min/week), i.e. a total of 300 min/week of physical activity/PE. The ASK study was implemented over 7 months, from November 2014 to June 2015. We assessed academic performance in reading, numeracy and English using Norwegian National tests delivered by The Norwegian Directorate for Education and Training. We assessed physical activity objectively at baseline, midpoint and at the end of the intervention. All other variables were measured at baseline and post-intervention. In addition, we used qualitative methodologies to obtain an in-depth understanding of children’s embodied experiences and pedagogical processes taking place during the intervention.DiscussionIf successful, ASK could provide strong evidence of a relation between physical activity and academic performance that could potentially inform the process of learning in elementary schools. Schools might also be identified as effective settings for large scale public health initiatives for the prevention of NCDs.Trial registrationClinicaltrials.gov ID nr: NCT02132494. Date of registration, 6th of May, 2014.

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Effects of duodenal switch alone or in combination with sleeve gastrectomy on body weight and lipid metabolism in rats

Cited by 3 publications

References 33 publications

Predictive associations between serum fatty acids and lipoproteins in healthy non-obese Norwegians: implications for cardiovascular health

Predictive associations between serum fatty acids and lipoproteins in healthy non-obese Norwegians: implications for cardiovascular health

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

Active Smarter Kids (ASK): Rationale and design of a cluster-randomized controlled trial investigating the effects of daily physical activity on children’s academic performance and risk factors for non-communicable diseases

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