In vitroeffects ofYunnan Baiyaoon canine hemangiosarcoma cell lines

Abstract

Yunnan Baiyao is a Chinese herbal medicine that has been utilized for its anti-inflammatory, haemostatic, wound healing and pain relieving properties in people. It has been utilized in the veterinary profession to control bleeding in dogs with hemangiosarcoma (HSA) and has been anecdotally reported to prolong survival times in dogs with this neoplasm. This study evaluated the in vitro activity of Yunnan Baiyao against three canine HSA cell lines after treatment with increasing concentrations of Yunnan Baiyao (50, 100, 200, 400, 600 and 800 µg mL(-1) ) at 24, 48 and 72 h. Mean half maximum inhibitory concentration (IC50 ) at 72 h for DEN, Fitz, SB was 369.9, 275.9 and 325.3 µg mL(-1) , respectively. Caspase-3/7 activity increased in correlation with the IC50 in each cell line which was confirmed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, APO-BRDU Kit; BD Biosciences, San Jose, CA, USA) assay. VEGF in cell supernatant was also quantified. Overall, the study found that Yunnan Baiyao causes dose and time dependent HSA cell death through initiation of caspase-mediated apoptosis, which supports future studies involving Yunnan Baiyao.

Use of Yunnan Baiyao and epsilon aminocaproic acid in dogs with right atrial masses and pericardial effusion

“…As such, 1 capsule twice daily requires only 1–2 packets of YB be dispensed for the dog to have sufficient medications until the recheck examination. YB was recently found to induce dose‐ and time‐dependent canine HSA cell death in vitro through initiation of caspase‐mediated apoptosis . This study demonstrated that the average inhibitory concentration of YB for 3 different canine HSA cell lines was 350.17 μg/mL .…”

“…YB was recently found to induce dose‐ and time‐dependent canine HSA cell death in vitro through initiation of caspase‐mediated apoptosis . This study demonstrated that the average inhibitory concentration of YB for 3 different canine HSA cell lines was 350.17 μg/mL . However, it is unclear how many capsules per day would be required to attain this inhibitory concentration in dogs.…”

“…The proposed mechanism is enhanced expression of surface glycoproteins on platelets under conditions of stimulation, which shortens bleeding and clotting times . In addition, YB has been found to induce dose‐ and time‐dependent canine HSA cell death in vitro through initiation of caspase‐mediated apoptosis …”

Long‐term postoperative effects of administration of allogeneic blood products in 104 dogs with hemangiosarcoma

“…To the best of authors' knowledge, this is the first published study to evaluate the clinical effects of Yunnan Baiyao in dogs with splenic HSA. Yunnan Baiyao slows the growth of cultured HSA cells in vitro, but little clinical data are available . A retrospective study of dogs with pericardial effusion from right atrial masses found that Yunnan Baiyao was not associated with a delay in recurrence of clinical signs after pericardiocentesis .…”

“…Yunnan Baiyao slows the growth of cultured HSA cells in vitro, but little clinical data are available. 43 A retrospective study of dogs with pericardial effusion from right atrial masses found that Yunnan Baiyao was not associated with a delay in recurrence of clinical signs after pericardiocentesis. 44 Our data provide evidence to support an association between prolonged DFI and Yunnan Baiyao administration.…”