Characterization and Molecular Mapping of a New Virescent Mutant in Rice

Zhang, Qunyu; Xue, Dexing; Li, Xiaoyü; Long, Yunming; Zeng, Xiaoqun; Liu, Yao-Guang

doi:10.1016/j.jgg.2014.01.010

Cited by 9 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chlorophyll a was reduced more than chlorophyll b (Table 1). The malfunctioning chloroplast and reduced chlorophyll biosynthesis in yellow young leaves seem to be a common feature for many temperature-dependent virescent mutants such as those found in Arabidopsis [20], cotton [32]; rice [7,18,26,33,34,35,36,37,38], maize [39], wheat [40], and apple [41]. Biswal et al [42] showed that the biosynthesis of chlorophyll a is closely linked to chloroplast development.…”

Section: Discussionmentioning

confidence: 99%

“…In the rice virescent mutant v14, the second and third leaves displayed an albino phenotype, but from the fourth leaf stage, all leaves including the albino ones are recovered to normal green [38]. The cells of the second and third leaves of the v14 mutant lack mature thylakoid membrane systems and starch grain but normal chloroplast structure can be observed in the fourth leaf of the same mutant.…”

Section: Discussionmentioning

confidence: 99%

“…The cells of the second and third leaves of the v14 mutant lack mature thylakoid membrane systems and starch grain but normal chloroplast structure can be observed in the fourth leaf of the same mutant. Zhang et al [38] reasoned that there may be different regulation pathways that are involved in chloroplast development in rice. Another possible explanation is that there might be other CsaCNGCs homologs with redundant functional activities in greening stages.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.)

Han

Zhang

Wang

et al. 2016

IJMS

View full text Add to dashboard Cite

Leaf color mutants are common in higher plants that can be used as markers in crop breeding or as an important tool in understanding regulatory mechanisms in chlorophyll biosynthesis and chloroplast development. In virescent leaf mutants, young leaves are yellow in color, which gradually return to normal green when the seedlings grow large. In the present study, we conducted phenotypic characterization and genetic mapping of the cucumber virescent leaf mutant 9110Gt conferred by the v-1 locus. Total chlorophyll and carotenoid content in 9110Gt was reduced by 44% and 21%, respectively, as compared with its wild type parental line 9110G. Electron microscopic investigation revealed fewer chloroplasts per cell and thylakoids per chloroplast in 9110Gt than in 9110G. Fine genetic mapping allowed for the assignment of the v-1 locus to a 50.4 kb genomic DNA region in chromosome 6 with two flanking markers that were 0.14 and 0.16 cM away from v-1, respectively. Multiple lines of evidence supported CsaCNGCs as the only candidate gene for the v-1 locus, which encoded a cyclic-nucleotide-gated ion channel protein. A single nucleotide change in the promoter region of v-1 seemed to be associated with the virescent color change in 9110Gt. Real-time PCR revealed significantly lower expression of CsaCNGCs in the true leaves of 9110Gt than in 9110G. This was the first report that connected the CsaCNGCs gene to virescent leaf color change, which provided a useful tool to establish linkages among virescent leaf color change, chloroplast development, chlorophyll biosynthesis, and the functions of the CsaCNGCs gene.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.)

Han

Zhang

Wang

et al. 2016

IJMS

View full text Add to dashboard Cite

show abstract

“…The V14 locus was previously mapped as a 162-kb region on chromosome 7, using a stage-conditional virescent - 14 ( v14 ) mutant of Taichung 65 (T65), a japonica cultivar [ 36 ]. This mutant develops albinism in the first two true leaves at 25 °C and returns green thereafter [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

A rice mTERF protein V14 sustains photosynthesis establishment and temperature acclimation in early seedling leaves

et al. 2021

View full text Add to dashboard Cite

Background Plant mitochondrial transcription termination factor (mTERF) family members play important roles in development and stress tolerance through regulation of organellar gene expression. However, their molecular functions have yet to be clearly defined. Results Here an mTERF gene V14 was identified by fine mapping using a conditional albino mutant v14 that displayed albinism only in the first two true leaves, which was confirmed by transgenic complementation tests. Subcellular localization and real-time PCR analyses indicated that V14 encodes a chloroplastic protein ubiquitously expressed in leaves while spiking in the second true leaf. Chloroplastic gene expression profiling in the pale leaves of v14 through real-time PCR and Northern blotting analyses showed abnormal accumulation of the unprocessed transcripts covering the rpoB-rpoC1 and/or rpoC1-rpoC2 intercistronic regions accompanied by reduced abundance of the mature rpoC1 and rpoC2 transcripts, which encode two core subunits of the plastid-encoded plastid RNA polymerase (PEP). Subsequent immunoblotting analyses confirmed the reduced accumulation of RpoC1 and RpoC2. A light-inducible photosynthetic gene psbD was also found down-regulated at both the mRNA and protein levels. Interestingly, such stage-specific aberrant posttranscriptional regulation and psbD expression can be reversed by high temperatures (30 ~ 35 °C), although V14 expression lacks thermo-sensitivity. Meanwhile, three V14 homologous genes were found heat-inducible with similar temporal expression patterns, implicating their possible functional redundancy to V14. Conclusions These data revealed a critical role of V14 in chloroplast development, which impacts, in a stage-specific and thermo-sensitive way, the appropriate processing of rpoB-rpoC1-rpoC2 precursors and the expression of certain photosynthetic proteins. Our findings thus expand the knowledge of the molecular functions of rice mTERFs and suggest the contributions of plant mTERFs to photosynthesis establishment and temperature acclimation.

show abstract

“…Many virescent leaf genes have been positional cloned in these plants. For examples, five rice virescent mutant genes have been cloned including V1 (Kusumi et al, 2011), V2 (Sugimoto et al, 2004;Sugimoto et al, 2007), V3 (Yoo et al, 2009), YSA (Su et al, 2012) and v14 (Zhang et al, 2014). Xing et al (2014) identified a virescent yellow-like (vyl) maize mutant and validated one of the proteolytic subunits of the chloroplast Clp protease complex (Chr.9_ClpP5 gene) as the candidate gene for vyl.…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping and Transcriptome Analysis of Virescent Leaf Gene v-2 in Cucumber (Cucumis sativus L.)

et al. 2020

View full text Add to dashboard Cite

Leaf color mutants are the ideal materials to explore the pathways of chlorophyll metabolism, chloroplast development and photosynthesis system. In this study, a new virescent leaf mutant 104Y was identified by spontaneous mutation, whose cotyledon and upper five true leaves were yellow color. The yellow true leaves gradually turned green from top to bottom with increased chlorophyll contents. Genetic analysis indicated that the virescent leaf was controlled by one single recessive gene v-2, which was accurately mapped into 36.0-39.7 Mb interval on chromosome 3 by using BSA-seq and linkage analysis. Fine mapping analysis further narrowed v-2 into 73-kb genomic region including eight genes with BC 1 and F 2 populations. Through BSA-seq and cDNA sequencing analysis, only one nonsynonymous mutation existed in the Csa3G890020 gene encoding auxin F-box protein was identified, which was predicted as the candidate gene controlling virescent leaf. Comparative transcriptome analysis and quantitative real-time PCR analysis revealed that the expression level of Csa3G890020 was not changed between EC1 and 104Y. However, RNA-seq analysis identified that the key genes involved in chlorophyll biosynthesis and auxin signaling transduction network were mainly down-regulated in 104Y compared with EC1, which indicated that the regulatory functions of Csa3G890020 could be performed at post-transcriptional level rather than transcriptional level. This is the first report to map-based clone an auxin F-box protein gene related to virescent leaf in cucumber. The results will exhibit a new insight into the chlorophyll biosynthesis regulated by auxin signaling transduction network.

show abstract

Characterization and Molecular Mapping of a New Virescent Mutant in Rice

Cited by 9 publications

References 20 publications

Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.)

Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.)

A rice mTERF protein V14 sustains photosynthesis establishment and temperature acclimation in early seedling leaves

Fine Mapping and Transcriptome Analysis of Virescent Leaf Gene v-2 in Cucumber (Cucumis sativus L.)

Contact Info

Product

Resources

About