Cefotaxime-ResistantSalmonella entericain Travelers Returning from Thailand to Finland

Gunell, Marianne; Aulu, Laura; Jalava, Jari; Lukinmaa-Åberg, Susanna; Österblad, Monica; Ollgren, Jukka; Huovinen, Pentti; Siitonen, Anja; Hakanen, Antti

doi:10.3201/eid2007.131744

Cited by 17 publications

(15 citation statements)

References 14 publications

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“…The occurrence of Salmonella serovar 4,[5],12:i:- constitutes an animal husbandry and public hygiene concern because the pathogenicity of these strains in farm animals and humans is considered to be comparable to that of Salmonella serovar Typhimurium [13, 19]. Additionally, resistance to antimicrobials, including third-generation cephalosporins and plasmid- mediated quinolone resistance (PMQR) genes, have been reported in Salmonella serovar 4,[5],12:i:- strains [18]. It is generally believed that one of the reasons for the increased prevalence of antimicrobial-resistant strains in food-producing animals is the usage of antimicrobials in rearing practices [43].…”

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confidence: 99%

Genotypic and phenotypic characterization of Salmonella enterica subsp. enterica serovar Typhimurium monophasic variants isolated in Thailand and Japan

Murase

Ozaki

Phuektes

2018

The Journal of Veterinary Medical Science

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Monophasic variants of Salmonella enterica serovar Typhimurium isolated in Thailand and Japan were characterized to elucidate the genetic basis of the monophasic phenotype, genetic relatedness, and antimicrobial resistance. A total of 20 Salmonella isolates agglutinated with anti-O4 and anti-H:i serum and not agglutinated with either anti-H:1 or anti-H:2 serum were identified as monophasic variants of Salmonella serovar Typhimurium because they harbored IS200, specific to this serovar, and lacked the fljB gene. An allele-specific PCR-based genotyping method that detects a clade-specific single nucleotide polymorphism indicated that seven swine isolates and one human isolate from Thailand were grouped into clade 1; five isolates from layer chicken houses and layer chicken feces from Japan were grouped into clade 8, together with two Salmonella serovar Typhimurium isolates from chicken houses in Japan; and five isolates from swine feces from Thailand and two isolates from layer chicken feces from Japan were grouped into clade 9. Multilocus sequencing typing demonstrated that sequence type (ST) 34 isolates were solely grouped into clade 9. Clade 1 and 8 isolates were assigned as ST19. Pulsed-field gel electrophoresis revealed multiple types within each of the clades. The presence of antimicrobial resistance genes and plasmid replicon type, of the clade 1 and 9 isolates were comparable to those reported for epidemic strains of monophasic variants. Our results suggest that monitoring monophasic variants of serovar Typhimurium is important for understanding of the spread of these variants in Thailand and Japan.

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confidence: 99%

Genotypic and phenotypic characterization of Salmonella enterica subsp. enterica serovar Typhimurium monophasic variants isolated in Thailand and Japan

Murase

Ozaki

Phuektes

2018

The Journal of Veterinary Medical Science

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“…Choleraesuis is reported to have evolved through gene deletion and sequence alternations to become a very efficient and successful pathogen among non-typhoidal Salmonella, particularly with regard to its pathogenicity and antimicrobial resistance [3,29]. These deletions and sequence alterations bring out ESBL and ampC genes, and qnr-related resistance due to their mobile nature, making the treatment for invasive Salmonella infections challenging [40]. The abundant use of antibiotics in human and veterinary medicine and in food production has led to antimicrobial drug resistance [41].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial profile of Salmonella enterica serotype Choleraesuis from free-range swine in Kakamega fish market, western Kenya

Onyango

Ndeda

Wandili

et al. 2014

J Infect Dev Ctries

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Introduction: Salmonella enterica subspecies enterica serovar Choleraesuis is a host-adapted, facultative, intracellular pathogen that causes swine paratyphoid. Its antimicrobial resistance presents a challenge to feed manufacturing industries. However, stopping antibiotics in animal feed would have economic implications for the industry. Methodology: Conventional microbial methods for isolation and identification of S. Choleraesuis were employed. The isolates were subjected to screening against 17 antimicrobial agents and genotyping of resistance markers by PCR. The data were then analyzed and presented in percentages. Results: Phenotypically, 43 out of 95 isolates showed multidrug resistance. Among the 17 antibiotics tested, resistance was observed as follows: sulphonamides (45

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“…Salmonella are gram-negative bacteria and common causes of diarrheal diseases affecting millions of people all over the world, and notably some resistant serotypes have emerged. Salmonella enterica serovar Typhimurium ( S. enterica serovar Typhimurium) is the most frequently reported serovar causing human salmonellosis worldwide ( Bugarel et al, 2012 ; Gunell et al, 2014 ). In recent decades, a monophasic variant of S. enterica serovar Typhimurium (named S. enterica serovar 4,5,12:i:-) that is deficient in the expression of the phase-2 flagellar antigen has emerged.…”

Section: Introductionmentioning

confidence: 99%

A Thermostable, Modified Cathelicidin-Derived Peptide With Enhanced Membrane-Active Activity Against Salmonella enterica serovar Typhimurium

Klubthawee

Aunpad

2021

Front. Microbiol.

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Foodborne illness caused by consumption of food contaminated with Salmonella is one of the most common causes of diarrheal disease and affects millions of people worldwide. The rising emergence and spread of antimicrobial resistance, especially in some serotypes of Salmonella, has raised a great awareness of public health issues worldwide. To ensure safety of the food processing chain, the development of new food preservatives must be expedited. Recently, thermal- and pH-stable antimicrobial peptides have received much attention for use in food production, and represent safe alternatives to chemical preservatives. A 12-mer cathelicidin-derived, α-helical cationic peptide, P7, displayed rapid killing activity, against strains of drug-resistant foodborne Salmonella enterica serovar Typhimurium and its monophasic variant (S. enterica serovar 4,5,12:i:-) and had minimal toxicity against mouse fibroblast cells. P7 tended to form helical structure in the membrane-mimic environments as evaluated by circular dichroism (CD) spectroscopy. The action mode of P7 at the membrane-level was affirmed by the results of flow cytometry, and confocal, scanning and transmission electron microscopy. P7 killed bacteria through binding to bacterial membranes, penetration and the subsequent accumulation in S. enterica serovar Typhimurium cytoplasm. This induced membrane depolarization, permeabilization, and sequential leakage of intracellular substances and cell death. Except for sensitivity to proteolytic digestive enzymes, P7 maintained its inhibitory activity against S. enterica serovar Typhimurium in the presence of different conditions [various salts, extreme pHs and heat (even at 100°C)]. Moreover, the peptide is unlikely to induce bacterial resistance in vitro. Taken together, this study demonstrated that the membrane-permeabilizing P7 peptide has much potential as a new antimicrobial agent for use in food processing and preservation.

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Cefotaxime-ResistantSalmonella entericain Travelers Returning from Thailand to Finland

Cited by 17 publications

References 14 publications

Genotypic and phenotypic characterization of <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Typhimurium monophasic variants isolated in Thailand and Japan

Genotypic and phenotypic characterization of <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Typhimurium monophasic variants isolated in Thailand and Japan

Antimicrobial profile of Salmonella enterica serotype Choleraesuis from free-range swine in Kakamega fish market, western Kenya

A Thermostable, Modified Cathelicidin-Derived Peptide With Enhanced Membrane-Active Activity Against Salmonella enterica serovar Typhimurium

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