Involvement of multiple phytoene synthase genes in tissue- and cultivar-specific accumulation of carotenoids in loquat

Fu, Xiumin; Feng, Chao; Wang, Chunyan; X, Yin; Lu, Pengjun; Grierson, Don; Xu, Changjie; Chen, Kunsong

doi:10.1093/jxb/eru257

Cited by 71 publications

(72 citation statements)

References 43 publications

(60 reference statements)

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“…JA2 and JA2L cause stomatal closure and opening, respectively (Du et al, 2014) while OsSWN1 and 2 control different aspects of secondary wall formation (Yoshida et al, 2013). Similarly, four phytoene synthase ( PSY ) genes of loquat function differently (Fu et al, 2014) though they cause carotenoid production. Penetration-resistance genes PEN1, PEN2 , and PEN3 function in overlapping as well as distinct manners in cell wall-based defense (Johansson et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Three Rice NAC Transcription Factors Heteromerize and Are Associated with Seed Size

et al. 2016

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NACs are plant-specific transcription factors (TFs) involved in multiple aspects of development and stress. In rice, three NAC TF encoding genes, namely ONAC020, ONAC026, and ONAC023 express specifically during seed development, at extremely high levels. They exhibit significantly strong association with seed size/weight with the sequence variations located in the upstream regulatory region. Concomitantly, their expression pattern/levels during seed development vary amongst different accessions with variation in seed size. The alterations in the promoter sequences of the three genes, amongst the five rice accessions, correlate with the expression levels to a certain extent only. In terms of transcriptional properties, the three NAC TFs can activate and/or suppress downstream genes, though to different extents. Only ONAC026 is localized to the nucleus while ONAC020 and ONAC023 are targeted to the ER and cytoplasm, respectively. Interestingly, these two proteins interact with ONAC026 and the dimers localize in the nucleus. Trans-splicing between ONAC020 and ONAC026 results in three additional forms of ONAC020. The transcriptional properties including activation, repression, subcellular localization and heterodimerization of trans-spliced forms of ONAC020 and ONAC026 are different, indicating toward their role as competitors. The analysis presented in this paper helps to conclude that the three NAC genes, which are associated with seed size, have independent as well as overlapping roles during the process and can be exploited as potential targets for crop improvement.

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Section: Introductionmentioning

confidence: 99%

Three Rice NAC Transcription Factors Heteromerize and Are Associated with Seed Size

et al. 2016

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“…Phytoene synthase (PSY) regulates carotenoid metabolic flux in the downstream enzymatic steps for the biosynthesis of lycopene, α-carotene and β-carotene (Sandmann et al, 2006; Cazzonelli and Pogson, 2010; Grassi et al, 2013; Fu et al, 2014). Many plant species are known to have multiple copies of PSY gene (Gallagher et al, 2004; Clotault et al, 2008; Giorio et al, 2008; Li et al, 2008; Fu et al, 2014; Ampomah-Dwamena et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Regulation of Banana Phytoene Synthase (MaPSY) Expression, Characterization and Their Modulation under Various Abiotic Stress Conditions

et al. 2017

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Phytoene synthase (PSY) is a key regulatory enzyme of carotenoid biosynthesis pathway in plants. The present study examines the role of PSY in carotenogenesis and stress management in banana. Germplasm screening of 10 Indian cultivars showed that Nendran (3011.94 μg/100 g dry weight) and Rasthali (105.35 μg/100 g dry weight) contained the highest and lowest amounts of β-carotene, respectively in ripe fruit-pulp. Nendran ripe pulp also showed significantly higher antioxidant activity as compared to Rasthali. Meta-analysis of three banana PSY genes (MaPSY1, MaPSY2, and MaPSY3) was performed to identify their structural features, subcellular, and chromosomal localization in banana genome. The distinct expression patterns of MaPSY1, MaPSY2, and MaPSY3 genes were observed in various tissues, and fruit developmental stages of these two contrasting cultivars, suggesting differential regulation of the banana PSY genes. A positive correlation was observed between the expression of MaPSY1 and β-carotene accumulation in the ripe fruit-peel and pulp of Nendran. The presence of stress responsive cis-regulatory motifs in promoter region of MaPSY genes were correlated with the expression pattern during various stress (abscisic acid, methyl jasmonate, salicylic acid and dark) treatments. The positive modulation of MaPSY1 noticed under abiotic stresses suggested its role in plant physiological functions and defense response. The amino acid sequence analysis of the PSY proteins in contrasting cultivars revealed that all PSY comprises conserved domains related to enzyme activity. Bacterial complementation assay has validated the functional activity of six PSY proteins and among them PSY1 of Nendran (Nen-PSY1) gave the highest activity. These data provide new insights into the regulation of PSY expression in banana by developmental and stress related signals that can be explored in the banana improvement programs.

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“…The researches to reveal the mechanism for the variation of carotenoid content have been done in maize and demonstrated that the genetic variation in the β-carotene hydroxylase gene -CR-TRB1 caused reduced transcript expression correlating with higher carotene concentrations (Yan et al 2010). In previous research, the alternative splicing of PSY gene affected the carotenoid contents (Fu et al 2014). In this study, the analysis of gene coding sequences showed that only two transcripts of ZDS genes were truncated.…”

Section: Discussionmentioning

confidence: 54%

“…Beta-carotene and lutein are the major carotenoids in the loquat flesh (Zhou et al 2007); the functional analysis of multiple phytoene synthase genes indicated that this gene family is involved in the accumulation of loquat carotenoids (Fu et al 2014). However, no thorough transcriptome information for red-fleshed and white-fleshed fruits has been provided for these two loquat accessions, which may reveal more clues for genes involved in carotenoid accumulation.…”

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confidence: 99%

Comparative transcriptomic analysis reveals a series of single nucleotide polymorphism between red- and white-fleshed loquats (Eriobotrya japonica)

Sun¹,

Li²,

Chen³

et al. 2017

CZECH J GENET PLANT

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Sun S., Li J., Chen D., Xie H., Tu M., Jia L., Jiang G. (2017): Comparative transcriptomic analysis reveals a series of single nucleotide polymorphism between red-and white-fleshed loquats (Eriobotrya japonica). Czech J. Genet. Plant Breed., 53: 97−106.Loquat (Eriobotrya japonica) is an economically important crop and red-fleshed cultivars have a much higher carotenoid content than white-fleshed cultivars. We used Illumina RNA-seq technology to gain a global overview of the loquat transcriptome from a mixture of fruit samples at different developmental stages for both red-fleshed and white-fleshed loquat. A total of 94.98 million paired-end short reads were obtained and 61 586 unigenes were generated from de novo assembly with an average length of 817 bp. Among these unigenes, 44 710 unigenes were annotated by blast against Nr, Swissprot, GO, COG and KEGG databases. For these annotated unigenes, 123 biosynthesis pathways were predicted by mapping these unigenes to the reference canonical pathways and 41 unigenes were predicted to be involved in carotenoid biosynthesis. RT-qPCR analysis showed that the expression level of the LCYB gene was higher in red-fleshed loquat and the CRTRB gene had a higher expression level in white-fleshed loquat. Comparative analysis of the two transcriptomes revealed 2396 single nucleotide polymorphisms (SNPs) between red-and white-fleshed loquats. The majority of SNPs identified between the two loquat cultivars were nonsense mutations and one out of eleven SNPs in candidate genes involved in carotenoid biosynthesis was a sense mutation. This suggests that the analysis based on transcriptomes can reveal key genes related to the carotenoid biosynthesis and more carotene in red-fleshed loquat cultivars may result from both more carotene produced by the higher expression of LCYB genes and less carotene converted because of the low expression of the CRTRB gene. All these results from the transcriptome analysis will be useful for the elucidation of genetic differences between red-and white-fleshed loquat fruits and further functional analysis for genes responsible for carotenoid accumulation.

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Involvement of multiple phytoene synthase genes in tissue- and cultivar-specific accumulation of carotenoids in loquat

Abstract: SummaryFour phytoene synthase genes and several variants were characterized, and their evolution and function in differential carotenoid accumulation in leaf, peel, and flesh of white- and red-fleshed loquats were established.

Cited by 71 publications

References 43 publications

Three Rice NAC Transcription Factors Heteromerize and Are Associated with Seed Size

Three Rice NAC Transcription Factors Heteromerize and Are Associated with Seed Size

Regulation of Banana Phytoene Synthase (MaPSY) Expression, Characterization and Their Modulation under Various Abiotic Stress Conditions

Comparative transcriptomic analysis reveals a series of single nucleotide polymorphism between red- and white-fleshed loquats (Eriobotrya japonica)

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