Eckols reduce dental pulp inflammation through the <scp>ERK</scp>1/2 pathway independent of <scp>COX</scp>‐2 inhibition

Paudel, Usha; Yh, Lee; Th, Kwon; Nh, Park; Bs, Yun; Ph, Hwang; Hk, Yi

doi:10.1111/odi.12266

Cited by 18 publications

(18 citation statements)

References 31 publications

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“…The RBL cells were stimulated by antigen-antibody reaction or A23187. According to previously reported phlorotannin concentration ranging 1 to 200 μM in cultured model cell experiments [7,8,10,26,27,28,29], we chose a concentration range from 10 to 200 μM in our experiments.…”

Section: Resultsmentioning

confidence: 99%

“…The same two phlorotannins at 100 μM suppressed COX-2 mRNA expression in RBL cells, compared with the other four phlorotannins (Figure 2). It has been reported that the anti-inflammatory effect of 6,6′-bieckol is via the inhibition of COX-2 expression and activity through suppression of NF-κB and ERK 1/2 signaling [28,29]. The suppression effect of 6,6′-bieckol on ear swelling could be attributable to greater suppression of COX-2 mRNA expression than to inhibition of COX-2 activity.…”

Section: Discussionmentioning

confidence: 99%

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Orally Administered Phlorotannins from Eisenia arborea Suppress Chemical Mediator Release and Cyclooxygenase-2 Signaling to Alleviate Mouse Ear Swelling

et al. 2018

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Phlorotannin is the collective term for polyphenols derived from brown algae belonging to the genera Ascopyllum, Ecklonia, Eisenia, Fucus and Sargassum etc. Since the incidence of allergies is currently increasing in the world, there is a focus on phlorotannins having anti-allergic and anti-inflammatory effects. In this study, six purified phlorotannins (eckol; 6,6′-bieckol; 6,8′-bieckol; 8,8′-bieckol; phlorofucofuroeckol (PFF)-A and PFF-B) from Eisenia arborea, orally administered to mice, were examined for their suppression effects on ear swelling. In considering the suppression, we also examined whether the phlorotannins suppressed release of chemical mediators (histamine, leukotriene B4 and prostaglandin E2), and mRNA expression and/or the activity of cyclooxygenase-2 (COX-2), using RBL-2H3 cells, a cultured mast cell model. Results showed that the phlorotnannins exhibited suppression effects in all experiments, with 6,8′-bieckol, 8,8′-bieckol and PFF-A showing the strongest of these effects. In conclusion, orally administered phlorotannins suppress mouse ear swelling, and this mechanism apparently involves suppression of chemical mediator release and COX-2 mRNA expression or activity. This is the first report of the anti-allergic effects of the orally administered purified phlorotannins in vivo. Phlorotannins show potential for use in functional foods or drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Orally Administered Phlorotannins from Eisenia arborea Suppress Chemical Mediator Release and Cyclooxygenase-2 Signaling to Alleviate Mouse Ear Swelling

et al. 2018

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“…This finding suggests that phelligridin D has antioxidant, as well as anti‐inflammatory properties, and is effective against chronic inflammation via downregulation of ICAM‐1 and VCAM‐1. COX‐2 is responsible for prostaglandin synthesis, and related to acute inflammation at the site of inflammation . On the other hand, some recent evidence focusing on the inhibition of COX‐2 is deleterious to cardiovascular patients, and carries the risk of upper gastrointestinal bleeding and perforation, because it increases thrombotic tendencies .…”

Section: Discussionmentioning

confidence: 99%

“…This experiment used 3‐9 passages for PDLCs from each individual. Mineralization experiments for alkaline phosphatase (ALP) and Alizarin red S staining were carried out on cells cultured in 50 μg/mL of ascorbic acid, 10 mmol/L of β‐glycerophosphate and 100 nmol/L of dexamethasone as previously described …”

Section: Methodsmentioning

confidence: 99%

Anti‐inflammatory character of Phelligridin D modulates periodontal regeneration in lipopolysaccharide‐induced human periodontal ligament cells

Kim

Takanche

Yun

et al. 2018

J of Periodontal Research

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“…Celecoxib (CLX), 4-[5-(4-methylphenyl)-3-trifluromethyl-1H -pyrazol -1-yl} benzenesulfon-amide is a cycloxygenase-2 (COX-2) inhibitor and widely used as analgesic and antiinflammatory (NSAID) in treatment of osteoarthritis, rheumatoid arthritis, familial adenomatous polyposis (FAP), primary dysmenorrhea and dental practice 1,2,3 .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2017

IJPSR

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ABSTRACT:One of the major challenges in pharmaceutical development is the poor dissolution performance of drugs. Celecoxib (CLX) is a poorly water soluble drug with its bioavailability being limited by its poor dissolution. In this study spray drying method was employed to prepare CLX: PVP K30:HPB (hydroxypropyl β-cyclodextrin) amorphous ternary system (ATS). Statistical experimental design was employed to investigate the combined effect of two experimental factors, i.e., % of polyvinayl pyrrolidone (PVP) K30 and % of HPB on saturation solubility (SS), dissolution efficiency (DE) and mean dissolution time (MDT), considered as the responses to be optimized. Design of experiment was used in the context of quality by design, which requires a multivariate approach for understanding the multifactorial relationships among experimental factors. Central composite design allowed for defining a design space. Desirability function was used to attain simultaneous optimization of all responses. The desired goals were achieved for SS, DE and MDT. Experimental values obtained from the optimized formulations were very close to the predicted values, thus confirming the validity of the generated mathematical model. These results demonstrated the effectiveness of the proposed jointed use of PVP K30 and HPB, as well as the usefulness of the multivariate approach for the preparation of ATS. Validated optimum ATS were characterized by DSC, XRD, SEM and particle size analysis. Characterization results confirmed the formation of amorphous ternary system with average particle size 727.9±260.6nm.

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Eckols reduce dental pulp inflammation through the ERK1/2 pathway independent of COX‐2 inhibition

Abstract: This study elucidates that EB1 and EB5 have different types of anti-inflammatory property and help in dentin formation. Therefore, these compounds derived from marine algae of E. bicyclis may be used as selective therapeutic strategies for pulpitis and oral diseases.

Cited by 18 publications

References 31 publications

Orally Administered Phlorotannins from Eisenia arborea Suppress Chemical Mediator Release and Cyclooxygenase-2 Signaling to Alleviate Mouse Ear Swelling

Orally Administered Phlorotannins from Eisenia arborea Suppress Chemical Mediator Release and Cyclooxygenase-2 Signaling to Alleviate Mouse Ear Swelling

Anti‐inflammatory character of Phelligridin D modulates periodontal regeneration in lipopolysaccharide‐induced human periodontal ligament cells

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