Regulation of Hfq mRNA and Protein Levels in Escherichia coli and Pseudomonas aeruginosa by the Burkholderia cenocepacia MtvR sRNA

Grilo, André; Sousa, Sílvia A.; Feliciano, Joana R.; Costa, Paulo J.; Leitão, Jorge H.

doi:10.1371/journal.pone.0098813

Cited by 10 publications

(12 citation statements)

References 39 publications

(70 reference statements)

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“…A wide range of bacterial species were investigated in these studies, including Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa, Vibrio cholera, Salmonella typhimurium, Enterococcus faecalis, Neisseria menin-gitidis, Staphylococcus aureus , and Clostridium acetobu-tylicum . Seven studies were performed on E. coli , 30 , 35 , 39 , 41 , 44 , three on Pseudomonads , 26 , 31 , 45 and two on Salmonella spp. 27 , 46 The remaining genera were examined by a single study.…”

Section: Resultsmentioning

confidence: 99%

“…Antibiotic resistance-encoding genes were either deleted or introduced into the experimental strains. 30 , 31 , 35 – 49 This was followed by overexpression of sRNAs of interest in order to detect changes of antibiotic susceptibility. 35 – 49 …”

Section: Resultsmentioning

confidence: 99%

“…The included studies have shown that regulated expression of bacterial sRNAs is important in sustaining antibiotic- resistant phenotypes and conferring bacterial survival advantage. 26 – 31 , 47 Interestingly, experimental alteration of antibiotic-responsive sRNAs considerably increased bacterial susceptibility to various antibiotics. 30 , 31 , 49 …”

Section: Resultsmentioning

confidence: 99%

“…The change in the expression of these sRNAs maintains protein synthesis and ribosomal function in the presence of antimicrobial challenge, thereby ensuring bacterial survival. 26 , 27 , 31 Upon the exposure of antibiotics, genes coding proteins including metabolism, transport, transcriptional regulation, and ribosome proteins are unregulated. 26 In the presence of tigecycline and tetracycline, sRNA sYJ20 was upregulated in Salmonella typhimurium .…”

Section: Discussionmentioning

confidence: 99%

“… 26 – 29 More importantly, overexpression of some of these sRNAs increases antibiotic susceptibility of the otherwise highly resistant bacterial strains. 30 , 31 In this review, we performed a systematic search of the literature to discuss the therapeutic potential of these sRNAs in combating against the big problem of multidrug-resistant bacterial infection.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Potential and use of bacterial small RNAs to combat drug resistance: a systematic review

Chan¹,

Ho²,

Liu³

et al. 2017

IDR

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BackgroundOver the decades, new antibacterial agents have been developed in an attempt to combat drug resistance, but they remain unsuccessful. Recently, a novel class of bacterial gene expression regulators, bacterial small RNAs (sRNAs), has received increasing attention toward their involvement in antibiotic resistance. This systematic review aimed to discuss the potential of these small molecules as antibacterial drug targets.MethodsTwo investigators performed a comprehensive search of MEDLINE, EmBase, and ISI Web of Knowledge from inception to October 2016, without restriction on language. We included all in vitro and in vivo studies investigating the role of bacterial sRNA in antibiotic resistance. Risk of bias of the included studies was assessed by a modified guideline of Systematic Review Center for Laboratory Animal Experimentation (SYRCLE).ResultsInitial search yielded 432 articles. After exclusion of non-original articles, 20 were included in this review. Of these, all studies examined bacterial-type strains only. There were neither relevant in vivo nor clinical studies. The SYRCLE scores ranged from to 5 to 7, with an average of 5.9. This implies a moderate risk of bias. sRNAs influenced the antibiotics susceptibility through modulation of gene expression relevant to efflux pumps, cell wall synthesis, and membrane proteins.ConclusionPreclinical studies on bacterial-type strains suggest that modulation of sRNAs could enhance bacterial susceptibility to antibiotics. Further studies on clinical isolates and in vivo models are needed to elucidate the therapeutic value of sRNA modulation on treatment of multidrug-resistant bacterial infection.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Potential and use of bacterial small RNAs to combat drug resistance: a systematic review

Chan¹,

Ho²,

Liu³

et al. 2017

IDR

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Research Progress on Antibacterial Application with Nucleic Acid and Nucleic Acid Materials

Sun

Lin

2021

Advances in Nanomaterials-Based Cell Biology Research

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Functional investigation of the chromosomal ccdAB and hipAB operon in Escherichia coli Nissle 1917

Xia

et al. 2020

Appl Microbiol Biotechnol

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Toxin-antitoxin systems (TASs) have attracted much attention due to their important physiological functions. These small genetic factors have been widely studied mostly in commensal Escherichia coli strains, whereas the role of TASs in the probiotic E. coli Nissle 1917 (EcN) is still elusive. Here, the physiological role of chromosomally encoded type II TASs in EcN was examined. We showed that gene pair ECOLIN_00240-ECOLIN_00245 and ECOLIN_08365-ECOLIN_08370 were two functional TASs encoding CcdAB and HipAB, respectively. The homologs of CcdAB and HipAB were more conserved in E. coli species belonging to pathogenic groups, suggesting their important roles in EcN. CRISPRi-mediated repression of ccdAB and hipAB significantly reduced the biofilm formation of EcN in the stationary phase. Moreover, ccdAB and hipAB were shown to be responsible for the persister formation in EcN. Biofilm and persister formation of EcN controlled by the ccdAB and hipAB were associated with the expression of genes involved in DNA synthesis, SOS response, and stringent response. Besides, CRISPRi was proposed to be an efficient tool in annotating multiple TASs simultaneously. Collectively, our results advance knowledge and understanding of the role of TASs in EcN, which will enhance the utility of EcN in probiotic therapy. Key points• Two TASs in EcN were identified as hipAB and ccdAB.• Knockdown of HipAB and CcdAB resulted in decreased biofilm formation of EcN.• Transcriptional silencing of hipAB and ccdAB affected the persister formation of EcN.• An attractive link between TASs and stress response was unraveled in EcN.• CRISPRi afforded a fast and in situ annotation of multiple TASs simultaneously.

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Regulation of Hfq mRNA and Protein Levels in Escherichia coli and Pseudomonas aeruginosa by the Burkholderia cenocepacia MtvR sRNA

Cited by 10 publications

References 39 publications

Potential and use of bacterial small RNAs to combat drug resistance: a systematic review

Potential and use of bacterial small RNAs to combat drug resistance: a systematic review

Research Progress on Antibacterial Application with Nucleic Acid and Nucleic Acid Materials

Functional investigation of the chromosomal ccdAB and hipAB operon in Escherichia coli Nissle 1917

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