Antioxidant and Hepatoprotective Activity of Veronica ciliata Fisch. Extracts Against Carbon Tetrachloride-Induced Liver Injury in Mice

Yin, Li; Wei, Lei; Fu, Rao; Ding, Li‐Sheng; Guo, Yiran; Tang, Lin; Chen, Fang

doi:10.3390/molecules19067223

Cited by 26 publications

(23 citation statements)

References 20 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2 b). This result confirms a previous report [ 9 ]. The analysis of the 9 sub-fractions (A − I) from the EtOAc fraction, showed that Fraction E possessed the greatest antioxidant activity (Fig.…”

Section: Resultssupporting

confidence: 94%

See 1 more Smart Citation

Bioactivity-guided isolation of antioxidant and anti-hepatocarcinoma constituents from Veronica ciliata

Yin

Tan

et al. 2016

Chemistry Central Journal

Self Cite

View full text Add to dashboard Cite

BackgroundVeronica ciliata Fisch., widely distributed in western China, has been traditionally used in Tibetan Medicine as a treatment for hepatitis, cholecystitis, rheumatism, and urticaria. However, V. ciliata Fisch. has not been subjected to detailed chemical constitution analysis and the bioactive studies were restricted to its crude extracts. It is necessary to investigate the active chemical components of these extracts and identify their biological effects.ResultsFour iridoid glycosides, (veronicoside, cataposide, amphicoside, and verminoside) were isolated from the ethyl acetate fraction. Among these compounds, veronicoside and verminoside were isolated for the first time from this plant. These compounds exhibited strong antioxidant activity and inhibitory activity on HepG2 cell proliferation. The antioxidant activity of verminoside was equal to Vc. Cataposide, amphicoside and verminoside had stronger anti-hepatocarcinoma activity than 5-fluorouracil.ConclusionsFour iridoid glycosides,(veronicoside, cataposide, amphicoside and verminoside) were isolated from the extract of V. ciliata Fisch. using bioassay-guided screening.Among these compounds, veronicoside and verminoside were isolated for the first time from this plant. The above results indicated that these compounds were the active chemical components responsible for the antioxidant and anti-hepatocarcinoma properties of V. ciliata Fisch. The underlying mechanism of their bioactivity is worthy of further investigation.Graphical abstractBioactivity-guided isolation of antioxidant and anti-hepatocarcinoma constituents from Veronica ciliata

show abstract

Section: Resultssupporting

confidence: 94%

“…The extracts of V. ciliata Fisch. were reported to have strong antioxidant activities and significantly protective effects against acute hepatotoxicity induced by CCl 4 [ 9 ]. Five iridoid glycosides and three derivatives of benzoic acid have been isolated from V. ciliata Fisch.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity-guided isolation of antioxidant and anti-hepatocarcinoma constituents from Veronica ciliata

Yin

Tan

et al. 2016

Chemistry Central Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, GSH combines with harmful substances produced by CCl 4 in the liver and reduces liver damage in order to protect the liver. Previous studies have indicated that GSH may be used to detect the liver damage caused by CCl 4 (23,24). Elevation of TG levels indicated higher fatty acid content, while in a clinical context very high levels of TG are usually associated with liver disease (25).…”

Section: Discussionmentioning

confidence: 99%

Preventive activity of banana peel polyphenols on CCl4-induced experimental hepatic injury in Kunming mice

Wang

Xia

Zhu

et al. 2016

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

The aim of the present study was to evaluate the preventive effects of banana peel polyphenols (BPPs) against hepatic injury. Mice were divide into normal, control, 100 mg/kg and 200 mg/kg banana peel polyphenol and silymarin groups. All the mice except normal mice were induced with hepatic damage using CCl. The serum and tissue levels of mice were determined by a kit and the tissues were further examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. BPPs reduced the serum levels of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase in a CCl-induced mouse model of hepatic injury. Furthermore, BPPs reduced the levels of malondialdehyde and triglyceride, while increasing glutathione levels in the serum and liver tissues of mice. In addition, the effects of 200 mg/kg treatment were more evident, and these effects were comparable to those of the drug silymarin. Serum levels of the cytokines, interleukin (IL)-6, IL-12, tumor necrosis factor (TNF)-α and interferon-γ, were reduced in the mice treated with BPPs compared with injury control group mice, and these levels were comparable to those of the normal and silymarin-treated groups. Histopathological examination indicated that BPPs were able to reduce the extent of CCl-induced liver tissue injury and protect the liver cells. Furthermore, the mRNA and protein expression levels of the inflammation-associated factors cyclooxygenase-2, nitric oxide synthase, TNF-α and IL-1β were reduced in mice treated with BPPs compared with the control group mice. Mice that received 200 mg/kg BPP exhibited reduced expression levels of these factors compared with mice that received 100 mg/kg BPP. In conclusion, the results of the present study suggested that BPPs exert a good preventive effect against hepatic injury.

show abstract

“… 1 Toxic chemicals, xenobiotics, alcohol consumption, malnutrition, anemia, medications, autoimmune disorders, and viral infections 2 , 3 are some major causes of liver disease; among which, medications are the most common contributing factors. 4 Drug-induced liver damage accounts for more than 60% of all cases in the United States of America, and it is the leading cause of acute liver failure. 5 …”

Section: Introductionmentioning

confidence: 99%

In vitro Antioxidant and in vivo Hepatoprotective Activities of Root Bark Extract and Solvent Fractions of Croton macrostachyus Hochst. Ex Del. (Euphorbiaceae) on Paracetamol-Induced Liver Damage in Mice

Tafere¹,

Tuem²,

Gebre³

et al. 2020

JEP

View full text Add to dashboard Cite

Background Liver disease is a major public health threat, particularly in developing countries. Several medicinal plants and formulations have been claimed to have liver protective activities. The present study aimed to evaluate in vitro antioxidant and in vivo hepatoprotective activities of root bark extracts of Croton macrostachyus (Euphorbiaceae). Methods Free radical scavenging activity of crude extract and solvent fractions of the plant was conducted using the DPPH assay method. Hepatoprotective activities of the crude extract and solvent fractions of the plant were carried out based on paracetamol-induced liver damage in mice. Serum biomarkers (AST, ALT, ALP, total bilirubin and total protein) were assessed to find out the effect. Histopathological examination was also carried out for all groups of mice to further confirm the findings. Results Antioxidant assay revealed that the crude extract, aqueous fraction and chloroform fraction of Croton macrostachyus exhibited free radical scavenging activity with IC 50 values of 128.6, 168.9, and 406 µg/mL, respectively. Pretreatment of the mice with the crude extract and solvent fractions of Croton macrostachyus significantly reduced ALP (p<0.001), ALT (p<0.001), and AST (p<0.001) levels at all the administered doses compared to the toxic group. The crude extract and chloroform fraction decreased total bilirubin level at doses of 200 mg/kg (P<0.05) and 400 mg/kg (P<0.001). Pretreatment of the mice with 400 mg/kg of the crude extract and aqueous fraction elevated total protein value compared to the paracetamol treated group (P<0.05). The hepatoprotective activities of the plant extracts were confirmed by histopathological studies. Conclusion From this study, it can be concluded that the crude extract and solvent fractions of Croton macrostachyus demonstrated antioxidant and hepatoprotective activities.

show abstract

Antioxidant and Hepatoprotective Activity of Veronica ciliata Fisch. Extracts Against Carbon Tetrachloride-Induced Liver Injury in Mice

Cited by 26 publications

References 20 publications

Bioactivity-guided isolation of antioxidant and anti-hepatocarcinoma constituents from Veronica ciliata

Bioactivity-guided isolation of antioxidant and anti-hepatocarcinoma constituents from Veronica ciliata

Preventive activity of banana peel polyphenols on CCl4-induced experimental hepatic injury in Kunming mice

<p>In vitro Antioxidant and in vivo Hepatoprotective Activities of Root Bark Extract and Solvent Fractions of <em>Croton macrostachyus</em> Hochst. Ex Del. (<em>Euphorbiaceae</em>) on Paracetamol-Induced Liver Damage in Mice</p>

Contact Info

Product

Resources

About