Function of mechanically lengthened jejunum after restoration into continuity

Sullins, Veronica F.; Wagner, Justin P.; Walthers, Christopher M.; Chiang, Elvin; Lee, Steven L.; Wu, Benjamin M.; Dunn, James C.Y.

doi:10.1016/j.jpedsurg.2014.01.033

Cited by 17 publications

(6 citation statements)

References 25 publications

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“…Additionally, increase in insulin-like growth factor (IGF-I) expression has been localized to the muscularis propria of lengthened segments and could account for thickened muscularis [ 38 ]. In prior studies, intestinal motility and absorptive function remained intact despite muscularis thickening in these animals [ 19 ]. Other studies have also demonstrated return to normal muscularis thickness once the physical force is removed [ 39 ].…”

Section: Discussionmentioning

confidence: 93%

Three-dimensionally printed surface features to anchor endoluminal spring for distraction enterogenesis

et al. 2018

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Spring-mediated distraction enterogenesis has been studied as a novel treatment for short bowel syndrome (SBS). Previous approaches are limited by multiple surgeries to restore intestinal continuity. Purely endoluminal devices require a period of intestinal attachment for enterogenesis. The purpose of this study is to modify the device to prevent premature spring migration in a porcine model. Two models were created in juvenile mini-Yucatan pigs for the placement of three-dimensionally printed springs. (1) Two Roux-en-y jejunojenostomies with two Roux limbs were made. A spring with bidirectional hooked surface features was placed in one Roux limb and a spring with smooth surface was placed in the other Roux limb. (2) The in-continuity model had both hooked and smooth surface springs placed directly in intestinal continuity. Spring location was evaluated by weekly radiographs, and the intestine was retrieved after 2 to 4 weeks. Springs with smooth surfaces migrated between 1 to 3 weeks after placement in both porcine models. Springs with bidirectional hooked surface features were anchored to the intestine for up to 4 weeks without migration. Histologically, the jejunal architecture showed significantly increased crypt depth and muscularis thickness compared to normal jejunum. Bidirectional features printed on springs prevented the premature migration of endoluminal springs. These novel spring anchors allowed for their endoluminal placement without any sutures. This approach may lead to the endoscopic placement of the device for patients with SBS.

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Section: Discussionmentioning

confidence: 93%

Three-dimensionally printed surface features to anchor endoluminal spring for distraction enterogenesis

et al. 2018

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“…To evaluate for absorption, we utilized a previously described method of isolated intestinal segments to measure carbohydrate and peptide absorption in SERTKO mice compared to WT (Sullins et al ). In our study we analyzed for the absorption of glucose and a fluorescent‐tagged peptide D‐Ala‐Lys‐AMCA, and found significant increases for both substrates in SERTKO mice compared to WT.…”

Section: Discussionmentioning

confidence: 99%

“…Glucose absorption in isolated and perfused intestinal segments was measured, as previously described (Sullins et al ). Briefly, three mice from each group were fasted overnight prior to induction of anesthesia and laparotomy in a warmed and humidified isolette.…”

Section: Methodsmentioning

confidence: 99%

Potentiated serotonin signaling in serotonin re‐uptake transporter knockout mice increases enterocyte mass and small intestinal absorptive function

2019

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Genetic knockout of the serotonin reuptake transporter (SERT) potentiates serotonin signaling and increases crypt-cell proliferation, neuroplasticity, and mucosal surface area. However, it remains unknown whether these changes occur throughout the small intestine and whether they increase nutrient absorption. We hypothesized that serotonin-mediated mucosal growth would occur throughout the intestine and would increase enterocyte mass and absorptive function. Following institutional approval, intestinal segments spanning the bowel were harvested from 10 to 12 week-old SERT knockout (SERTKO) and wild-type (WT) C57Bl/6 mice. Histologic sections were used to measure villus height (VH), crypt depth (CD), and crypt proliferation index (CPI). Plasma citrulline was measured colorimetrically. Glucose and peptide absorption in isolated segments of small bowel were calculated using a previously described method for quantification after luminal instillation of substrate. At baseline, morphometric (VH/CD) and proliferative (CPI) parameters varied from jejunum to ileum. Enhanced 5-HT signaling significantly increased plasma citrulline levels and morphometric/proliferative parameters in all regions analyzed. Glucose absorption in WT mice varied throughout the small intestine, and SERTKO mice demonstrated significant increases in the middle and distal bowel. WT peptide absorption was similar throughout the small bowel, and SERTKO mice had significant increases in the proximal and distal bowel. Enhanced serotonin signaling results in increased morphometric and proliferative parameters throughout the small intestine, and results in increased enterocyte mass and intestinal absorptive function. These data further advance the concept that the serotonin system is an attractive therapeutic target for increasing functional intestinal mucosa.

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“…As we and others have previously shown, distraction enterogenesis produces significant, sustained intestinal lengthening with preservation of intestinal function [11][12][13]. This is characterized by mesenteric neovascularization, increased villus height and crypt depth, muscular hypertrophy, and increased epithelial cell proliferation [9,14,15].…”

Section: Introductionmentioning

confidence: 89%

A novel double-balloon catheter device for fully endoluminal intestinal lengthening

et al. 2014

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Function of mechanically lengthened jejunum after restoration into continuity

Cited by 17 publications

References 25 publications

Three-dimensionally printed surface features to anchor endoluminal spring for distraction enterogenesis

Three-dimensionally printed surface features to anchor endoluminal spring for distraction enterogenesis

Potentiated serotonin signaling in serotonin re‐uptake transporter knockout mice increases enterocyte mass and small intestinal absorptive function

A novel double-balloon catheter device for fully endoluminal intestinal lengthening

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