New CODIS core loci allele frequencies for 96,400 Brazilian individuals

Aguiar, Vitor R. C.; Castro, A.; Almeida, Vanessa C.O.; Malta, Frederico Scott Varella; Ferreira, Alexandre de Aguiar; Louro, Iúri Drumond

doi:10.1016/j.fsigen.2014.04.015

Cited by 15 publications

(4 citation statements)

References 12 publications

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“…Capillary electrophoresis was performed in an ABI 3500 instrument (Life Technologies, 36-cm array, POP-4, Data Collection v1.2, GeneMapper ID-X v1.4). Maternity Indexes were calculated based on Brazilian allelic frequencies published elsewhere (Aguiar et al, 2014;Hessab et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Research Article Resolving a human identification case for the Rio de Janeiro Police with massively parallel sequencing of mtDNA using a proposed pipeline

Bottino¹,

Silva²

2021

Genet. Mol. Res.

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The forensic community is concerned about the quality control of mitotypes reported, as probability estimates may be biased to the disadvantage of a suspect when there is incomplete data. This is particularly important when using new technology such as Massively Parallel Sequencing (MPS), which performs simultaneous analysis of millions of DNA fragments, generating vast amounts of data from small amounts of sample, providing more extensive genetic information. The use of MPS for complex forensic evidence analysis could save time and resources, and provide reliable data for extremely degraded DNA samples. However, data analysis methods can be challenging to implement in a forensic labs routine. Here, we propose a reliable and straightforward pipeline for mtDNA data analysis in forensic samples using MPS, and we used this pipeline to solve a human identification case by Rio de Janeiro Police who had recovered a severely putrefied body that could not be identified by conventional autosomal STR analysis. Mitochondrial DNA (mtDNA) from samples were sequenced on Ion Torrent PGM. Raw data from the sequencing was analyzed in two main pipeline protocols: quality control and forensic analysis. The first includes the analyses of raw ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 20 (1): gmr18737 C. Bottino et al. 2data and contamination control. The second generates haplotypes, allowing haplogroup classification, and biogeographic inference, as well as verification of a matrilineal relationship. This straightforward freeware pipeline for mtDNA data analysis should facilitate the implementation of this type of system in forensic lab routine. Using our pipeline, the definition of the samples being of African origin was more precise when considering the whole mtGenome and not only the control region, which is usually used for routine forensic analysis. The final analysis was consistent with the existence of a matrilineal relationship between the alleged son (bone sample recovered from the sea) and the mother.

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Section: Methodsmentioning

confidence: 99%

Research Article Resolving a human identification case for the Rio de Janeiro Police with massively parallel sequencing of mtDNA using a proposed pipeline

Bottino¹,

Silva²

2021

Genet. Mol. Res.

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“…Forensic anthropology teams from the Medical Examiner's Office André Roquette (IMLAR) conducted procedures related to the primary identification, including sampling for DNA analysis. Simultaneously, other steps had to be taken in the criminal investigation: such as confirmation of death; determination of the cause of death; and determination of aggravating or qualifying circumstances that played a part in the victim's death [8].…”

Section: Post Mortem Samplesmentioning

confidence: 99%

“…A forensic geneticist was in attendance at the collection center for consultations during the course of AM sampling. Before sampling the families, the collection team had already provided elimination samples (voluntarily collected DNA samples from individuals not involved in the alleged crime), which were input in the CODIS (Combined DNA Index System database from FBI) elimination category [8]. Whatman TM EasiCollect, devices from GE Healthcare which were previously assigned to collect samples of convicted offenders to input in CODIS database were redirected to the sampling of DVI AM samples.…”

Section: Ante-mortem (Am) Samplingmentioning

confidence: 99%

“…We used the software Familías version 3.2.5 to achieve statistical confirmation of the matches and to calculate likelihood ratio (LR) to indicate the strength of evidence, using a compiled database [8] [13] for allele frequency. The DNA laboratory team chose not to use the prior probability since part of this event could be categorized as an open event and then it was impossible to estimate the number of victims.…”

Section: Dna Matching and Statistical Analysismentioning

confidence: 99%

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Challenges in Implementing DVI Best Practice Guidelines Due to the Brumadinho Disaster

Silva,

Morais,

Silva

et al. 2024

FMAR

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The broke of a tailings dam from Vale S.A. destroyed a gigantic area killing 270 persons in Brumadinho/Brazil. Organizing activities after a mass disaster is a complex process that requires the involvement of many people and resources in the laboratory. To DNA identification of the victims, a daunting work had to be made by DNA laboratory staff in a collaborative effort with many partners. Efforts to implement good practice guidelines in Disaster Victim Identification (DVI) have revealed several important aspects that need to change in the forensic DNA laboratory. This article highlights the challenges of implementing DVI best practice guidelines in resource-poor settings, but with professionals from different sectors engaged in the same goal of briefly identifying victims and helping families and society.

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Population genetic study of a Peruvian population using human identification STRs

et al. 2020

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New CODIS core loci allele frequencies for 96,400 Brazilian individuals

Cited by 15 publications

References 12 publications

Research Article Resolving a human identification case for the Rio de Janeiro Police with massively parallel sequencing of mtDNA using a proposed pipeline

Research Article Resolving a human identification case for the Rio de Janeiro Police with massively parallel sequencing of mtDNA using a proposed pipeline

Challenges in Implementing DVI Best Practice Guidelines Due to the Brumadinho Disaster

Population genetic study of a Peruvian population using human identification STRs

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