Tissue-engineered cartilage for facial plastic surgery

Watson, Deborah; Reuther, Marsha S.

doi:10.1097/moo.0000000000000068

Cited by 17 publications

(17 citation statements)

References 39 publications

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“…Engineered tissue incorporated with cells and biomaterials has been spotlighted as an ideal graft due to the high regenerative efficiency resulting from the delivered cells. 23,32,33 However, to date, there are few studies on the development of engineered tissue with patient-specific design because of the difficulties in generating human-scale living tissue constructs. 34 As to the current limitations, the process presented here for generating patient-specific nasal cartilage is substantially different from the conventional approaches.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional printing of a patient-specific engineered nasal cartilage for augmentative rhinoplasty

Choi

Jung

et al. 2019

J Tissue Eng

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Autologous cartilages or synthetic nasal implants have been utilized in augmentative rhinoplasty to reconstruct the nasal shape for therapeutic and cosmetic purposes. Autologous cartilage is considered to be an ideal graft, but has drawbacks, such as limited cartilage source, requirements of additional surgery for obtaining autologous cartilage, and donor site morbidity. In contrast, synthetic nasal implants are abundantly available but have low biocompatibility than the autologous cartilages. Moreover, the currently used nasal cartilage grafts involve additional reshaping processes, by meticulous manual carving during surgery to fit the diverse nose shape of each patient. The final shapes of the manually tailored implants are highly dependent on the surgeons’ proficiency and often result in patient dissatisfaction and even undesired separation of the implant. This study describes a new process of rhinoplasty, which integrates three-dimensional printing and tissue engineering approaches. We established a serial procedure based on computer-aided design to generate a three-dimensional model of customized nasal implant, and the model was fabricated through three-dimensional printing. An engineered nasal cartilage implant was generated by injecting cartilage-derived hydrogel containing human adipose-derived stem cells into the implant containing the octahedral interior architecture. We observed remarkable expression levels of chondrogenic markers from the human adipose-derived stem cells grown in the engineered nasal cartilage with the cartilage-derived hydrogel. In addition, the engineered nasal cartilage, which was implanted into mouse subcutaneous region, exhibited maintenance of the exquisite shape and structure, and striking formation of the cartilaginous tissues for 12 weeks. We expect that the developed process, which combines computer-aided design, three-dimensional printing, and tissue-derived hydrogel, would be beneficial in generating implants of other types of tissue.

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Section: Discussionmentioning

confidence: 99%

Three-dimensional printing of a patient-specific engineered nasal cartilage for augmentative rhinoplasty

Choi

Jung

et al. 2019

J Tissue Eng

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“…Nasal chondrocytes (NCs) (Bujia, 1995;Kafienah et al, 2002;Richmon et al, 2005) have been used to engineer cartilaginous constructs both in vitro and in vivo (Asawa et al, 2009;Candrian et al, 2008;Chia et al, 2005;Fulco et al, 2014;Hellingman et al, 2011;Malda et al, 2004;Richmon et al, 2005;Rotter et al, 2001Rotter et al, , 2002Scotti et al, 2012;Tsaryk et al, 2017;Twu et al, 2014;Watson and Reuther, 2014) and have recently been explored for nucleus pulposus tissue regeneration (Tsaryk et al, 2015;Tsaryk et al, 2017). However, the response of nasal chondrocytes to oxygen concentration and growth factors in maintaining phenotype and optimal NP-like matrix formation remains largely unknown in 3D culture.…”

Section: Introductionmentioning

confidence: 99%

In vitro extracellular matrix accumulation of nasal and articular chondrocytes for intervertebral disc repair

Vedicherla

Buckley

2017

Tissue and Cell

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Chondrocyte based regenerative therapies for intervertebral disc repair such as Autologous Disc Cell Transplantation (ADCT, CODON) and allogeneic juvenile chondrocyte implantation (NuQu®, ISTO Technologies) have demonstrated good outcomes in clinical trials. However concerns remain with the supply demand reconciliation and issues surrounding immunoreactivity which exist for allogeneic-type technologies. The use of stem cells is challenging due to high growth factor requirements, regulatory barriers and differentiation towards a stable phenotype. Therefore, there is a need to identify alternative non-disc cell sources for the development and clinical translation of next generation therapies for IVD regeneration. In this study, we compared Nasal Chondrocytes (NC) as a non-disc alternative chondrocyte source with Articular Chondrocytes (AC) in terms of cell yield, morphology, proliferation kinetics and ability to produce key extracellular matrix components under 5% and 20% oxygen conditions, with and without exogenous TGF-β supplementation.Results indicated that NC maintained proliferative capacity with high amounts of sGAG and lower collagen accumulation in the absence of TGF-β supplementation under 5% oxygen conditions. Importantly, osteogenesis and calcification was inhibited for NC when cultured in IVD-like microenvironmental conditions. The present study provides a rationale for the exploration of nasal chondrocytes as a promising, potent and clinically feasible autologous cell source for putative IVD repair strategies.

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“…Autologous cartilage is preferred for grafting of cartilaginous defects over synthetic and allogenic grafts, as it avoids immune rejection risks and foreign body reactions. 1 Several research groups are moving toward tissue engineering autologous tracheal and bronchial cartilage. [2][3][4][5][6][7][8][9][10] In these studies, involving sheep, mouse, rat, and human tissue, progress has been made in decellularizing tracheal scaffolds, optimizing bioreactor conditions, investigating novel methods of tissue assembly, and determining the most appropriate cell sources.…”

mentioning

confidence: 99%

“…Autologous cartilage is preferred for grafting of cartilaginous defects over synthetic and allogenic grafts, as it avoids immune rejection risks and foreign body reactions. 1…”

mentioning

confidence: 99%

Compositional Assessment of Human Tracheal Cartilage by Infrared Spectroscopy

Linkov

Hanifi

Yousefi

et al. 2018

Otolaryngol.--head neck surg.

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Objectives To assess the potential of infrared fiber-optic spectroscopy to evaluate the compositional properties of human tracheal cartilage. Study Design Laboratory-based study. Methods Twenty human cadaveric distal tracheas were harvested (age range 20-78 years; 6 females, 14 males) for compositional analysis. Histologic staining, Fourier transform infrared imaging spectroscopy data on collagen and proteoglycan (PG) content, and near-infrared (NIR) fiber-optic probe spectroscopic data that reflect protein and water content were evaluated. NIR fiber-optic probe data were also obtained from the proximal trachea in 4 human cadavers (age range 51-65 years; 2 females, 2 males) in situ for comparison to distal trachea spectral data. Results In the distal trachea cohort, the spectroscopic-determined ratio of PG/amide I, indicative of the relative amount of PG, was significantly higher in the tissues from the younger group compared to the older group (0.37 ± 0.08 vs 0.32 ± 0.05, P = .05). A principal component analysis of the NIR spectral data enabled separation of spectra based on tracheal location, likely due to differences in both protein and water content. The NIR-determined water content based on the 5200-cm peak was significantly higher in the distal trachea compared to the proximal trachea ( P < .001). Conclusions Establishment of normative compositional values and further elucidating differences between the segments of trachea will enable more directed research toward appropriate compositional end points in regenerative medicine for tracheal repair.

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Tissue-engineered cartilage for facial plastic surgery

Cited by 17 publications

References 39 publications

Three-dimensional printing of a patient-specific engineered nasal cartilage for augmentative rhinoplasty

Three-dimensional printing of a patient-specific engineered nasal cartilage for augmentative rhinoplasty

In vitro extracellular matrix accumulation of nasal and articular chondrocytes for intervertebral disc repair

Compositional Assessment of Human Tracheal Cartilage by Infrared Spectroscopy

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