Effects of varying tissue sizes on the efficiency of baboon ovarian tissue vitrification

Lu, Xi-Lan; Yu, Jing; Zhang, Gang; Wei, Zengtao; Li, Juntao; Zhang, Jianmin

doi:10.1016/j.cryobiol.2014.05.006

Cited by 11 publications

(5 citation statements)

References 31 publications

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“…It has been reported that exposure of follicles to cryoprotectant agents could compromise their rates of survival (Rodrigues et al., ,b), even without freezing (Tsuribe, Gobbo, & da Cruz Landim‐Alvarenga, ). To improve vitrification, some modifications have been applied by researchers, which were the changes in the concentration and type of cryoprotectant (Herraiz et al., ; Lunardi et al., ; Yamaji et al., ), time (Ting, Yeoman, Lawson, & Zelinski, ), temperature (Mouttham & Comizzoli, ), the device used (Herraiz et al., ; Lunardi et al., ) and tissue size (Lu et al., ). Here, we noticed that follicular viability as a whole has been reduced in all of the groups after two weeks of tissue culture.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Mohammadzadeh

Safdarian

Amidi

et al. 2017

Reprod Domestic Animals

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The aim of this study was to evaluate the effects of the two types of media, namely minimum essential medium (αMEM) and Ham'sF10, supplemented with foetal bovine serum (FBS) or bovine serum albumin (BSA) in vitrification/warming solution on the quality and viability of sheep ovarian follicles. Vitrification method was applied for cryopreservation of sheep ovarian cortex using Ham'sF10 and αMEM supplemented with either BSA or FBS. There were five groups: Fresh, Ham'sF10+ BSA, Ham'sF10+ FBS, αMEM + BSA and αMEM + FBS. Samples were cultured for two weeks after warming. Viability and morphology of follicles and DNA fragmentation in follicles and in tissue stroma cells were analysed before vitrification/warming and following one and two weeks of culture. The Ham'sF10+ FBS and Ham'sF10+ BSA groups showed a significant decrease in follicular viability after one week of culture (p < .05 vs. Fresh). Following two weeks of culture, all groups revealed a considerable fall in the number of viable follicles (p < .05 vs. Fresh). There was an increase in DNA fragmentation of connective tissue cells but not in the follicles (p < .05). Our results showed the better application of αMEM supplemented with BSA as a vitrification solution in improvement of cryopreservation effects and maintenance of follicular survival.

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Section: Discussionmentioning

confidence: 99%

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Mohammadzadeh

Safdarian

Amidi

et al. 2017

Reprod Domestic Animals

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“…37, No. 2, June 2022 110 Prather, 2011), but the challenges to preserve genetic material from female is not sufficient to reflect its demands compared to other animal species, such as sheep (Fathi et al, 2011), goat (Rodrigues et al, 2004), cow (Kagawa et al, 2009), dog (Brandao et al, 2021, mouse (Salehnia et al, 2002;Terraciano et al, 2020), and non-human primate (Nyachieo et al, 2013;Lu et al, 2014). Successful establishment of cryopreservation system using female genetic material has many benefits in case of animal species especially valuable transgenic animal (Takeo et al, 2020) and endangered animal (Santos et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Fertility preservation in pig using ovarian tissues by vitrification method

Hwang

2022

J Anim Reprod Biotechnol

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Cryopreservation of porcine ovarian tissue by vitrification method is a promising approach to preserve genetic materials for future use. However, information is not enough and technology still remains in a challenge stage in pig. Therefore, the objective of present study was to determine possibility of vitrification method to cryopreserve porcine ovarian tissue and to confirm an occurrence of cryoinjuries. Briefly, cryoinjuries and apoptosis patterns in vitrified-warmed ovarian tissue were examined by histological evaluation and TUNEL assay respectively. In results, a damaged morphology of oocytes was detected among groups and the rate was significantly (p < 0.05) lower in vitrification group (25.8%) than freezing control group (67.7%), while fresh control group (6.6%) showed significantly (p < 0.05) lower than both groups. In addition, cryoinjury that form a wave pattern of tissues around follicles was found in the frozen control group, but not in the fresh control group as well as in the vitrification group. Apoptotic cells in follicle was observed only in freezing control group while no apoptotic cell was found in both fresh control and vitrification. Similarly, apoptotic patterns of tissues not in follicle were comparable between fresh control and vitrification groups while freezing control group showed increased tendency. Conclusively, it was confirmed that vitrification method has a prevention effect against cryoinjury and this method could be an alternative approach for cryopreservation of genetic material in pigs. Further study is needed to examine the viability of oocytes derived from vitrified-warmed ovarian tissue.

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“…The hormonal activity of thawed and cultured follicles were selected as an evaluation criteria for effectiveness of cryopreservation protocols. Steroidogenic activity of ovarian tissue and isolated follicles is know as an indicator of tissue viability [ 39 , 43 ]. In our study we found an enhanced P4 production during culture time and enhanced secretion of E2 with further decline.…”

Section: Discussionmentioning

confidence: 99%

Innovative multi-protectoral approach increases survival rate after vitrification of ovarian tissue and isolated follicles with improved results in comparison with conventional method

et al. 2018

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BackgroundIn recent years, autotransplantation of cryopreserved ovarian tissue became a promising approach to preserve female fertility. The slow freezing is the most effective technique which resulted in greater live birth incidence so far. Despite that, interest to vitrification of the ovarian tissue is swiftly growing, thereby undermining the necessity for further improvements in the technique. In present study, we evaluated possibilities to increase follicle survival rates adopting innovative multi-protectoral vitrification protocols, applied to the slivers of ovarian cortex or isolated early-antral follicles, frozen individually. These experimental protocols have been compared with with validated vitrification and slow freezing ones, clinically used for female fertility preservation.ResultsThe results showed that third tested variation of experimental vitrification protocol, with four cryoprotectants in relatively low concentrations and applied to pieces of ovarian tissue at 0 °C during equilibration, increased survival rate of ovine ovarian tissue and improved results in comparison with conventional vitrification method. This variation of experimental protocol showed significant increase in percentage of follicles with good morphology (69,3%) in comparison with only commercially available vitrification protocol for ovarian tissue (62,1%). Morphology results were confirmed by TUNEL assay. Analysis of estradiol and progesterone production by cultured individual follicles after freezing/thawing revealed that steroids secretion remained significantly higher after multi-protectoral vitrification and slow freezing protocol, when follicles after standard vitrification protocol demonstrated decline in steroidogenic activity.ConclusionsThe multi-protectoral approach represents a workable solution to improve vitrification outcome on ovarian tissue and isolated follicles. The reduction of individual cryoprotectants concentrations, while maintaining their sufficient cumulative level in the final freezing solution, helps to increase efficiency of the procedure. Moreover, equilibration with lower temperatures helped to decrease even further the toxic effects of cryoprotectants and preserve original quality of ovarian tissue. Therefore, multi-protectoral vitrification can be suggested as an improved method for the clinical cryopreservation of ovarian tissue.

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Effects of varying tissue sizes on the efficiency of baboon ovarian tissue vitrification

Cited by 11 publications

References 31 publications

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Fertility preservation in pig using ovarian tissues by vitrification method

Innovative multi-protectoral approach increases survival rate after vitrification of ovarian tissue and isolated follicles with improved results in comparison with conventional method

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