Quorum quenching is an antivirulence strategy employed by endophytic bacteria

Kusari, Parijat; Kusari, Souvik; Lamshöft, Marc; Sezgin, Selahaddin; Spiteller, Michael; Kayser, Oliver

doi:10.1007/s00253-014-5807-3

Cited by 66 publications

(46 citation statements)

References 35 publications

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“…Similar studies looking at culturable bacterial endophytes identified nearly a dozen isolates from the Bacillus clade and two mycobacteria 1 . Of those Bacillus species, B. subtilis , B. lichenoformis and B. pumilis have been isolated as endophytes and have been shown to be beneficial to growth in other plant species 12– 14 .…”

Section: Introductionmentioning

confidence: 83%

“…However, many plant-associated microbes may provide benefits for plant cultivation in terms of growth stimulation, insect or microbial resistance, or may simply be neutral passengers 1– 3 . The microbiome of Cannabis leaves and flowers includes bacteria and fungi residing on the exterior surface of these tissues (epiphytes) as well as those residing within the plant tissues (endophytes).…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic analysis of medicinal Cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests

et al. 2016

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Background: The presence of bacteria and fungi in medicinal or recreational Cannabis poses a potential threat to consumers if those microbes include pathogenic or toxigenic species. This study evaluated two widely used culture-based platforms for total yeast and mold (TYM) testing marketed by 3M Corporation and Biomérieux, in comparison with a quantitative PCR (qPCR) approach marketed by Medicinal Genomics Corporation. Methods: A set of 15 medicinal Cannabis samples were analyzed using 3M and Biomérieux culture-based platforms and by qPCR to quantify microbial DNA. All samples were then subjected to next-generation sequencing and metagenomics analysis to enumerate the bacteria and fungi present before and after growth on culture-based media. Results: Several pathogenic or toxigenic bacterial and fungal species were identified in proportions of >5% of classified reads on the samples, including Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, Ralstonia pickettii, Salmonella enterica, Stenotrophomonas maltophilia, Aspergillus ostianus, Aspergillus sydowii, Penicillium citrinum and Penicillium steckii. Samples subjected to culture showed substantial shifts in the number and diversity of species present, including the failure of Aspergillus species to grow well on either platform. Substantial growth of Clostridium botulinum and other bacteria were frequently observed on one or both of the culture-based TYM platforms. The presence of plant growth promoting (beneficial) fungal species further influenced the differential growth of species in the microbiome of each sample. Conclusions: These findings have important implications for the Cannabis and food safety testing industries.

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Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis of medicinal Cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests

et al. 2016

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“…Such approaches are used to provide a holistic understanding of the distribution, biosynthesis, transport, and storage of target and/or selected non-target compounds with limited sample preparation [32]. For example, we used a combination of high-performance liquid chromatography-high-resolution mass spectrometry and MALDI-HRMS imaging to reveal that specific potent endophytic bacteria harbored in Cannabis sativa L. plants use quorum quenching as a possible antivirulence strategy to disrupt the cell-to-cell quorum-sensing signals in the biosensor strain, Chromobacterium violaceum [33]. More recently, using a similar approach, we revealed that the potent anticancer and cytotoxic compound maytansine is biosynthesized by the root-associated endophytic microorganisms of Putterlickia plants and is then further localized, typically within the root cortex, for a variety of ecological functions [34].…”

Section: Introductionmentioning

confidence: 99%

Spatial chemo-profiling of hypericin and related phytochemicals in Hypericum species using MALDI-HRMS imaging

et al. 2015

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Advanced analytical imaging techniques, including matrix-assisted laser desorption/ionization high-resolution mass spectrometry (MALDI-HRMS) imaging, can be used to visualize the distribution, localization, and dynamics of target compounds and their precursors with limited sample preparation. Herein we report an application of MALDI-HRMS imaging to map, in high spatial resolution, the accumulation of the medicinally important naphthodianthrone hypericin, its structural analogues and proposed precursors, and other crucial phytochemical constituents in the leaves of two hypericin-containing species, Hypericum perforatum and Hypericum olympicum. We also investigated Hypericum patulum, which does not contain hypericin or its protoforms. We focused on both the secretory (dark glands, translucent glands, secretory canals, laminar glands, and ventral glands) and the surrounding non-secretory tissues to clarify the site of biosynthesis and localization of hypericin, its possible precursors, and patterns of localization of other related compounds concomitant to the presence or absence of hypericin. Hypericin, pseudohypericin, and protohypericin accumulate in the dark glands. However, the precursor emodin not only accumulates in the dark glands but is also present outside the glands in both hypericin-containing species. In hypericin-lacking H. patulum, however, emodin typically accumulates only in the glands, thereby providing evidence that hypericin is possibly biosynthesized outside the dark glands and thereafter stored in them. The distribution and localization of related compounds were also evaluated and are discussed concomitant to the occurrence of hypericin. Our study provides the basis for further detailed investigation of hypericin biosynthesis by gene discovery and expression studies.

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“…Endophytic Bacillus isolates from Cannabis sativa quenched four different AHLs used by Chromobacterium violaceum in violacein production regulation (Kusari et al, 2014). Extracts of endophyte bacteria Bacillus and Enterobacter from Pterocarpus showed AHLs degrading ability when applied on biosensor strains (Rajesh, Ravishankar, 2014).…”

Section: Occurrence and Diversity Of Bacterial Endophytes In Agricultmentioning

confidence: 99%

Bacterial endophytes in agricultural crops and their role in stress tolerance: a review

Miliūtė

Buzaitė

Baniulis

et al. 2015

Zemdirbyste-Agriculture

197

112

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Bacterial endophytes are a class of endosymbiotic microorganisms widespread among plants that colonize intercellular and intracellular spaces of all plant compartments and do not cause plant disease or significant morphological changes. Plant and endophytic bacteria association includes vast diversity of bacterial taxa and plant hosts and in this review we present an overview of taxonomic composition of endophytes identified in common agricultural crops. Further, during the last decade, new aspects of the microbial diversity have emerged with application of new metagenomic analysis methods in studies of bacterial endophytes. Endophytic bacteria community structure is influenced by plant genotype, abiotic and biotic factors such as environment conditions, microbe -microbe interactions and plant -microbe interactions. Agricultural practices, such as soil tillage, irrigation, use of pesticides and fertilizers have a major effect on function and structure of soil and endophytic microbial populations. Therefore, the use of agricultural practices that maintain natural diversity of plant endophytic bacteria is becoming an important element of sustainable agriculture that could ensure plant productivity and quality of agricultural production. The diverse endophytic microbial communities play integral and unique role in the functioning of agroecosystems. Endophytic bacteria have been shown to have several beneficial effects on their host plant, including growth promoting activity, modulation of plant metabolism and phytohormone signalling that leads to adaptation to environmental abiotic or biotic stress. Use of endophytic bacteria presents a special interest for development of agricultural applications that ensure improved crop performance under cold, draught or contaminated soil stress conditions or enhanced disease resistance.

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Quorum quenching is an antivirulence strategy employed by endophytic bacteria

Cited by 66 publications

References 35 publications

Metagenomic analysis of medicinal Cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests

Metagenomic analysis of medicinal Cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests

Spatial chemo-profiling of hypericin and related phytochemicals in Hypericum species using MALDI-HRMS imaging

Bacterial endophytes in agricultural crops and their role in stress tolerance: a review

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