Pharmacological blockade of the MaxiK channel attenuates experimental acute pancreatitis and associated lung injury in rats

Ren, Jiandong; Xing, Yong-Jun; Fan, Kun; Bian, Yu; Jin, Weihua; Jiang, Yan; Li, Jing; Xue-chai, WU; Wang, Shihua; Wu, Juan; Chen, Hua

doi:10.1016/j.intimp.2014.04.003

Cited by 8 publications

(4 citation statements)

References 28 publications

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“…Soluble heparan sulphate, a glycosaminoglycan associated with nucleated cellular components and extracellular matrices, was released by proteases digestion in the pancreas. The rapid enzymatic degradation of heparan sulphate activated TLR 4 in PMBCs and led to SAP aggravation, even resulted in death . The second possible explanation contributed to the inducible formation of Card9‐Bcl10 complex was observed in SAP patients, which may be responsible for the NF‐κB and MAPK activation in PMBCs.…”

Section: Discussionmentioning

confidence: 99%

The role of Card9 overexpression in peripheral blood mononuclear cells from patients with aseptic acute pancreatitis

Yang

Weng

Wang

et al. 2015

J Cellular Molecular Medi

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Activated mononuclear cells are an early event in the course of severe acute pancreatitis (SAP). To date, the molecular mechanism triggering peripheral blood mononuclear cells (PBMCs) is poorly understood. The aim of this paper was to determine the potential role of Card9 in SAP. We collected data from 72 subjects between January 2013 and June 2014. Subsequently, PBMCs were isolated on day 1, 3 and 5 of pancreatitis. Immunofluorescence staining, quantitative real‐time PCR, Western blotting, immunoprecipitation and ELISA were used to determine the role of Card9 in SAP. Microbial culture showed that SAP patients at the early period did not develop any bacteria and fungi infection. Card9 expression in SAP patients was higher than that in mild acute pancreatitis and volunteer healthy controls, up to the peak on day 1. The monocyte‐derived cytokines interleukin (IL)‐17, IL‐1β, IL‐6 and tumour necrosis factor‐α mediated by the induction of Card9 markedly increased in SAP patients compared with the control group. Furthermore, the inducible formation of Card9‐Bcl10 complex was found in PBMCs, which may be involved in nuclear factor kappa B (NF‐κB) and p38 activation in SAP. Receiver operating characteristic curve indicated that Card9 levels had a high sensitivity of 87.5% and specificity of 67.7%, showing the close correlation with SAP patients. Card9 overexpression was firstly found in aseptic SAP, which may be played an important role in NF‐κB and p38 activation in PBMCs. It also provided the new insights into therapeutic interventions by targeting monocytes activation in SAP patients.

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Section: Discussionmentioning

confidence: 99%

The role of Card9 overexpression in peripheral blood mononuclear cells from patients with aseptic acute pancreatitis

Yang

Weng

Wang

et al. 2015

J Cellular Molecular Medi

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“…The neutrophil infiltration and the increased levels of proinflammatory cytokines in the lungs aggravated the ALI. The currently available data concerning the model of SAP-associated ALI, including the increased pulmonary inflammatory cell infiltration, and alveolar wall thickening, coupled with the elevation of proinflammatory cytokines, was indicative of the development of ALI after sodium taurocholate induction [ 39 – 41 ]. Treatment with SSBE as demonstrated in the present study attenuated the pathological pulmonary injury scores, the wet/dry weight ratio, MPO activity, and proinflammatory cytokines in the lungs, indicating that SSBE reduced the severity of sodium taurocholate-induced lung injury.…”

Section: Discussionmentioning

confidence: 99%

Involvement of the PI3K/Akt/NF-κB Signaling Pathway in the Attenuation of Severe Acute Pancreatitis-Associated Acute Lung Injury by Sedum sarmentosum Bunge Extract

Jin

Liu

Chen

et al. 2017

BioMed Research International

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Sedum sarmentosum Bunge possesses excellent anti-inflammatory properties and was used in the treatment of inflammatory diseases. The aim of the present study was to investigate the efficiency of Sedum sarmentosum Bunge extract (SSBE) on severe acute pancreatitis-associated (SAP-associated) acute lung injury (ALI) in rats and to explore the underlying mechanisms. Here, we used a sodium taurocholate-induced SAP rat model to determine the role of SSBE in ALI. During the course of pancreatitis, the expressions of phosphorylated phosphoinositide 3-kinases (PI3K)/protein kinase B (Akt) and nuclear factor-kappa B (NF-κB) p65 in the lungs were upregulated. Meanwhile, a parallel increase in the levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in the lungs was observed after the induction of SAP. Treatment with SSBE significantly reduced the expression of p-Akt and p-p65 in the lungs and attenuated the severity of SAP-associated ALI compared to the SAP group at 12 h and 24 h. In summary, this study showed that SSBE has beneficial effects on SAP-associated ALI, probably through the PI3-K/Akt signaling pathways by suppressing the NF-κB activities.

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“…The mortality rate of AP is severe when the condition progress to lung injury followed by multiple organ dysfunctions . The intricate balance of cytokine production relates the degree of tissue damage …”

Section: Introductionmentioning

confidence: 99%

“…[2][3][4][5] The intricate balance of cytokine production relates the degree of tissue damage. [6][7][8] Though several mediators like tumour necrosis factoralpha (TNF-a), interleukin-1 beta (IL-1b) have been investigated for their prominent role in inflammation, other gaseous mediators which are also reported to play a modulatory role in inflammation like hydrogen sulfide (H 2 S), nitric oxide (NO) and carbon monoxide (CO) are less explored. [9,10] However, intrinsically synthesized H 2 S is extensively studied because of its part as a pro-inflammatory mediator.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of methylsulfonylmethane in caerulein-induced acute pancreatitis and associated lung injury in mice

Velusamy

Tamizhselvi

2018

Journal of Pharmacy and Pharmacology

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Pharmacological blockade of the MaxiK channel attenuates experimental acute pancreatitis and associated lung injury in rats

Cited by 8 publications

References 28 publications

The role of Card9 overexpression in peripheral blood mononuclear cells from patients with aseptic acute pancreatitis

The role of Card9 overexpression in peripheral blood mononuclear cells from patients with aseptic acute pancreatitis

Involvement of the PI3K/Akt/NF-κB Signaling Pathway in the Attenuation of Severe Acute Pancreatitis-Associated Acute Lung Injury by Sedum sarmentosum Bunge Extract

Protective effect of methylsulfonylmethane in caerulein-induced acute pancreatitis and associated lung injury in mice

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