Elements of margin of safety, toxicity and action of sodium selenite in a lipopolysaccharide rat model

Forceville, Xavier; Touati, Samia; Toumelin, Philippe Le; Ducros, Véronique; Laporte, François; Chancerelle, Yves; Agay, Diane

doi:10.1016/j.jtemb.2014.03.010

Cited by 11 publications

(13 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the small differences between deficiency and toxic selenium excess, high availability of this element may negatively affect the cells. Among all known compounds of selenium, sodium selenite(IV) exhibits the highest toxicity [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Selenium on Morphological Changes in Candida utilis ATCC 9950 Yeast Cells

Kieliszek

Błażejak

Bzducha-Wróbel

et al. 2015

Biol Trace Elem Res

View full text Add to dashboard Cite

This paper presents the results of microscopic examinations of the yeast cells cultured in yeast extract–peptone–dextrose (YPD) media supplemented with sodium selenite(IV). The analysis of the morphological changes in yeast cells aimed to determine whether the selected selenium doses and culturing time may affect this element accumulation in yeast cell structures in a form of inorganic or organic compounds, as a result of detoxification processes. The range of characteristic morphological changes in yeasts cultivated in experimental media with sodium selenite(IV) was observed, including cell shrinkage and cytoplasm thickening of the changes within vacuole structure. The processes of vacuole disintegration were observed in aging yeast cells in culturing medium, which may indicate the presence of so-called ghost cells lacking intracellular organelles The changes occurring in the morphology of yeasts cultured in media supplemented with sodium selenite were typical for stationary phase of yeast growth. From detailed microscopic observations, larger surface area of the cell (6.03 μm2) and yeast vacuole (2.17 μm2) were noticed after 24-h culturing in the medium with selenium of 20 mg Se4+/L. The coefficient of shape of the yeast cells cultured in media enriched with sodium selenite as well as in the control YPD medium ranged from 1.02 to 1.22. Elongation of cultivation time (up to 48 and 72 h) in the media supplemented with sodium selenite caused a reduction in the surface area of the yeast cell and vacuole due to detoxification processes.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of Selenium on Morphological Changes in Candida utilis ATCC 9950 Yeast Cells

Kieliszek

Błażejak

Bzducha-Wróbel

et al. 2015

Biol Trace Elem Res

View full text Add to dashboard Cite

show abstract

“…The general toxicity is a threat. The toxicity is characterized by cardio-respiratory distress symptoms similar to those of sepsis [30,56]. In addition Na 2 SeO 3 cytotoxity seems increased in sepsis as observed in a rat lipopolysaccharide (LPS) model.…”

Section: Discussionmentioning

confidence: 97%

“…SOFA score, cardiac and respiratory SOFA scores, and plasma lactate also have a similar evolution. Cardio-respiratory distress is a critical point of acute Na 2 SeO 3 toxicity, which is concentration and dose dependent [30,56]. There is therefore no argument in favor of a toxic effect of continuous infusion of Na 2 SeO 3 at a dose corresponding to 4 mg Se the first day.…”

Section: Discussionmentioning

confidence: 99%

Delayed increase of plasma selenoproteins and absence of side effect induced by infusion of pharmacological dose of sodium selenite in septic shock: Secondary analysis of a multicenter, randomized controlled trial

Forceville

Laviolle²,

Gromadzińska³

et al. 2022

Journal of Trace Elements in Medicine and Biology

Self Cite

View full text Add to dashboard Cite

“…70 Nevertheless, so far there are no definitive data about toxicity limits of sodium selenite in the critically ill despite numerous RCTs performed in different ICU patient populations performed without sufficient preclinical data in humans. 71 Therefore, before new well-powered RCTs may be conducted, more well-designed pharmacokinetic and safety studies giving high-dose sodium selenite monotherapy, particularly in patients with renal dysfunction, and exploring long-term toxicity are warranted. In Table 1, data about the lowest observed adverse effect level (LOAEL) and the no observed adverse effect level (NOAEL) for selenium are shown.…”

Section: Pharmacokinetics and Safety Of Selenium In Icu Patientsmentioning

confidence: 99%

“…In addition, a single dose of 250 mg is necessary to induce acute toxicity, named selenosis 70 . Nevertheless, so far there are no definitive data about toxicity limits of sodium selenite in the critically ill despite numerous RCTs performed in different ICU patient populations performed without sufficient preclinical data in humans 71 . Therefore, before new well‐powered RCTs may be conducted, more well‐designed pharmacokinetic and safety studies giving high‐dose sodium selenite monotherapy, particularly in patients with renal dysfunction, and exploring long‐term toxicity are warranted.…”

Section: Pharmacokinetics and Safety Of Selenium In Icu Patientsmentioning

confidence: 99%

Pharmaconutrition With Selenium in Critically Ill Patients

2014

View full text Add to dashboard Cite

Selenium is a component of selenoproteins with antioxidant, anti-inflammatory, and immunomodulatory properties. Systemic inflammatory response syndrome (SIRS), multiorgan dysfunction (MOD), and multiorgan failure (MOF) are associated with an early reduction in plasma selenium and glutathione peroxidase activity (GPx), and both parameters correlate inversely with the severity of illness and outcomes. Several randomized clinical trials (RCTs) evaluated selenium therapy as monotherapy or in antioxidant cocktails in intensive care unit (ICU) patient populations, and more recently several meta-analyses suggested benefits with selenium therapy in the most seriously ill patients. However, the largest RCT on pharmaconutrition with glutamine and antioxidants, the REducing Deaths due to Oxidative Stress (REDOXS) Study, was unable to find any improvement in clinical outcomes with antioxidants provided by the enteral and parenteral route and suggested harm in patients with renal dysfunction. Subsequently, the MetaPlus study demonstrated increased mortality in medical patients when provided extra glutamine and selenium enterally. The treatment effect of selenium may be dependent on the dose, the route of administration, and whether administered with other nutrients and the patient population studied. Currently, there are few small studies evaluating the pharmacokinetic profile of intravenous (IV) selenium in SIRS, and therefore more data are necessary, particularly in patients with MOD, including those with renal dysfunction. According to current knowledge, high-dose pentahydrate sodium selenite could be given as an IV bolus injection (1000-2000 µg), which causes transient pro-oxidant, cytotoxic, and anti-inflammatory effects, and then followed by a continuous infusion of 1000-1600 µg/d for up to 10-14 days. Nonetheless, the optimum dose and efficacy still remain controversial and need to be definitively established.

show abstract

Elements of margin of safety, toxicity and action of sodium selenite in a lipopolysaccharide rat model

Abstract: Mechanisms of LPS and Na2SeO3 toxicity differ (i.e. NO, lactate). In septic shock 5H2O·Na2SeO3 toxicity increased, margin of safety decrease, but IP administration of dose considered as oxidant of 5H2O·Na2SeO3 showed beneficial effects.

Cited by 11 publications

References 58 publications

Effects of Selenium on Morphological Changes in Candida utilis ATCC 9950 Yeast Cells

Effects of Selenium on Morphological Changes in Candida utilis ATCC 9950 Yeast Cells

Delayed increase of plasma selenoproteins and absence of side effect induced by infusion of pharmacological dose of sodium selenite in septic shock: Secondary analysis of a multicenter, randomized controlled trial

Pharmaconutrition With Selenium in Critically Ill Patients

Contact Info

Product

Resources

About