Interceptor effect of C60 fullerene on the in vitro action of aromatic drug molecules

Abstract

C60 fullerenes are spherical molecules com posed purely of carbon atoms. They inspire a particularly strong scientific interest because of their specific physico chemical properties and potential medical and nanotech nological applications. In this work we are focusing on studying the influence of the pristine C60 fullerene on bio logical activity of some aromatic drug molecules in human buccal epithelial cells. Assessment of the heterochromatin structure in the cell nucleus as well as the barrier function of the cell membrane was performed. The methods of cell microelectrophoresis and atomic force microscopy were also applied. A concentration-dependent restoration of the functional activity of the cellular nucleus after exposure to DNA-binding drugs (doxorubicin, proflavine and ethidium bromide) has been observed in human buccal epithelial cells upon addition of C60 fullerene at a concentration of -1СГ5 M. The results were shown to follow the framework of interceptor/protector action theory, assuming that noncovalent complexation between C60 fullerene and the drugs (i.e., hetero-association) is the major process responsible for the observed biological effects. An independent con firmation of this hypothesis was obtained via investigation of the cellular response of buccal epithelium to the coad ministration of the aromatic drugs and caffeine, and it is based on the well-established role of hetero-association in drug-caffeine systems. The results indicate that C60 fuller ene may reverse the effects caused by the aromatic drugs, thereby pointing out the potential possibility of the use of aromatic drugs in combination with C60 fullerene for regu lation of their medico-biological action.

Influence of C(60) fullerene on the ischemia-reperfusion injury in the skeletal muscle of rat limb: mechanokinetic and biochemical analysis

“…Thus, C 60 FAS is a polydisperse colloidal nanosystem. This result is in a good agreement with our previous probe microscopic data which directly correlate with C 60 fullerene bioactivity [28,29].…”

Hetero-association of aromatic molecules in aqueous solution

“…in reduction of the consequences of overdosing of drugs during chemotherapy [42,52] or in the production of antimutagenic effects in vivo [53][54][55]. The recent in vitro study of cellular effects of a mixture of doxorubicin with fullerene [56], complemented by physico-chemical investigation of their complexation in physiological media [57,58], has shown that the effect of hetero-association may potentially lie behind new applications of fullerene molecules as elements of combinational chemotherapy of cancer.…”

Water around fullerene shape amphiphiles: A molecular dynamics simulation study of hydrophobic hydration

“…solubility, aggregation, and solvation of fullerenes are directly related to their functions in biosystems. [8][9][10][11][12][13][14][15][16][17][18][19][20] In a liquid environment-polar or non-polar-the control over the generation of fullerene clusters is a challenging multiscale problem that simultaneously involves a hierarchy of processes on very broad time and length scales. [21][22][23][24][25][26][27][28] Thus, in-depth knowledge of the origin, magnitude, and sign of solvent-mediated interaction from the molecular standpoint is of vital importance for the investigation of aggregation processes which begin on a single molecular level with the formation of a contact fullerene pair.…”