Expressional regulation of key hepatic enzymes of intermediary metabolism in European seabass (Dicentrarchus labrax) during food deprivation and refeeding

Viegas, Iván; Caballero‐Solares, Albert; Rito, João; Giralt, Marina; Pardal, Miguel Ângelo; Metón, Isidoro; Jones, John G.; Baanante, Isabel V.

doi:10.1016/j.cbpa.2014.04.004

Cited by 7 publications

(5 citation statements)

References 44 publications

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“…Total RNA extraction, reverse transcription, amplification by RT-PCR, RT-PCR product analysis and purification, transformation in competent cells, sequencing and design of specific oligonucleotides for quantitative real-time RT-PCR were conducted, as described previously, for GK and G6Pase ( 11 ) , and cytosolic alanine aminotransferase ( 20 ) . To our knowledge, no sequence was available for GDH, so for its partial sequencing, we followed the procedure described by Viegas and colleagues ( 11 , 20 ) . Amplification of GDH by RT-PCR was carried out using the pairs of primers designed from highly conserved regions of nucleotide sequences published in the GenBank for other fish species (Table 2).…”

Section: Methodsmentioning

confidence: 99%

Contribution of dietary starch to hepatic and systemic carbohydrate fluxes in European seabass (Dicentrarchus labraxL.)

et al. 2015

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In the present study, the effects of partial substitution of dietary protein by digestible starch on endogenous glucose production were evaluated in European seabass (Dicentrarchus labrax). The fractional contribution of dietary carbohydrates v. gluconeogenesis to blood glucose appearance and hepatic glycogen synthesis was quantified in two groups of seabass fed with a diet containing 30 % digestible starch (DS) or without a carbohydrate supplement as the control (CTRL). Measurements were performed by transferring the fish to a tank containing water enriched with 5 % 2 H 2 O over the last six feeding days, and quantifying the incorporation of 2 H into blood glucose and hepatic glycogen by 2 H NMR. For CTRL fish, gluconeogenesis accounted for the majority of circulating glucose while for the DS fish, this contribution was significantly lower (CTRL 85 (SEM 4) % v. DS 54 (SEM 2) %; P,0·001). Hepatic glycogen synthesis via gluconeogenesis (indirect pathway) was also significantly reduced in the DS fish, in both relative (CTRL 100 (SEM 1) % v. DS 72 (SEM 1) %; P, 0·001) and absolute terms (CTRL 28 (SEM 1) v. DS 17 (SEM 1) mmol/kg per h; P,0·001). A major fraction of the dietary carbohydrates that contributed to blood glucose appearance (33 (SEM 1) % of the total 47 (SEM 2) %) had undergone exchange with hepatic glucose 6-phosphate. This indicated the simultaneous activity of hepatic glucokinase and glucose 6-phosphatase. In conclusion, supplementation of digestible starch resulted in a significant reduction of gluconeogenic contributions to systemic glucose appearance and hepatic glycogen synthesis. Key words:2 H 2 O: Gluconeogenesis: Endogenous glucose production: Glucokinase: Starch utilisationIn aquaculture, substituting costly fishmeal with less expensive plant-derived carbohydrate (CHO) reduces feed input costs. The provision of dietary CHO to carnivorous fish such as the European seabass (Dicentrarchus labrax L.) may also have environmental benefits since CHO utilisation can potentially spare the catabolism of dietary amino acids to glucose and nitrogenous waste (1) . Dietary CHO influences growth, feed utilisation and deposition of nutrients according to species, quantity, origin and pre-treatment for improving digestibility (2 -4) . The effects of partial substitution of dietary protein by plant-derived CHO on food conversion efficiency and nutrient digestibility sources have been tested in seabass (5 -7) . In addition, its effects on energy retention and maintenance requirements (8) as well as the activity of key CHO-metabolising enzymes (6,9) have been studied. These studies have suggested that, on the one hand, carnivorous fish are able to up-regulate their capacity for hepatic glucose utilisation through the increased expression of gateway enzymes such as glucokinase when fed with a high-CHO diet. However, on the other hand, supplementation of fish feed with up to 30 % starch has not been shown to alter the overall protein efficiency ratio or nitrogen retention in seabass (7) . This is despite...

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Section: Methodsmentioning

confidence: 99%

Contribution of dietary starch to hepatic and systemic carbohydrate fluxes in European seabass (Dicentrarchus labraxL.)

et al. 2015

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“…alanine aminotransferase, ALT), better feed conversion ratio due to the increment of digestive enzyme activities, promotion of protein synthesis and enhancing synthesis and release of appetite and growth regulatory hormones (e.g. insulin-like growth factors, ghrelin, and leptin) (Gaylord and Gatlin, 2001;Ali et al, 2003;Won and Borski, 2013;Viegas et al, 2014;Bertucci et al, 2019). On the other hand, it has been demonstrated that feed deprivation may induce oxidative stress in fish by overproduction of reactive oxygen species (ROS) and decreasing the antioxidant reserves in tissues, especially in the liver that will, in turn, lead to disturbing the effective neutralization of ROS (Morales et al, 2004;Pascual et al, 2003;Pérez-Jiménez et al, 2007).…”

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Effects of a single-phase fasting period and subsequent re-feeding on compensatory growth, digestive enzyme activities, and antioxidant capacity of sobaity (Sparidentex hasta) and yellowfin seabream (Acanthopagrus latus)

Mozanzadeh

Safari

Ghaedi

et al. 2022

Annals of Animal Science

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An eight-week research was carried out to examine the influence of fasting (FA) and refeeding (RF) episodes on the compensatory growth responses (CGR) in sobaity (Sparidentex hasta, 10 g) and yellowfin seabreams (Acanthopagrus latus, 4.3 g) juveniles. Fish were fed with a commercial feed (contained 500 g kg−1 crude protein and 150 g kg−1 crude lipid) as following regimes: control (C, fish were fed three times every day), T1 (two weeks of feeding, one week of FA, and five weeks of RF), T2 (one week of feeding, two weeks of FA and five weeks of RF) and T3 (three weeks of FA and five weeks of RF). Two hundred and forty S. hasta juveniles were stocked into twelve 300-L tanks (20 fish tank−1), and 360 A. latus juveniles were allocated into other 12 tanks (30 fish tank−1). Each treatment was carried out in triplicates for each species, and each tank held only one of the species. The experiment was carried out for both species simultaneously. The weight and length of fish from the four groups were measured individually after the third week (after FA episode) and after eight weeks (after RF episode). After finishing the RF episode (eighth week), six fish of each tank were sacrificed with an overdose of 2-phenoxyethanol (1000 mg L−1), and the liver and the whole gut of the sacrificed fish were sampled, dissected, and then kept in a freezer (−80 °C) until further analyses. Survival rate was decreased in S. hasta juveniles with increasing the FA period mainly due to their cannibalistic behavior, which was triggered by starvation, but it was not affected in A. latus. The fasted groups in both species were significantly lost their weight after FA episodes. After five weeks of RF, S. hasta showed full compensatory growth response; meanwhile A. latus had a partial compensatory response (P<0.05). Hepatosomatic index value decreased after the FA period in both species, but it was restored to the normal level after RF phase. The activities of liver catalase, superoxide dismutase, glutathione-S-transferase, and glutathione peroxidase were increased in T2 group in S. hasta, but liver antioxidant enzymes were not affected in A. latus. In both species, the amount of the lipid peroxidation was significantly increased in the liver of fish groups subjected to T2 or T3 compared to T1 and control groups (P < 0.05). Liver alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in S. hasta fasted for two weeks were higher than the other groups. The activities of trypsin, chymotrypsin, α-amylase, and lipase in S. hasta fasted for a week (T1) were higher than control. In addition, the activities of trypsin and chymotrypsin in A. latus fasted for two or three weeks were lower than C and T1 groups. The activity of ALP was increased with increasing FA period in both species. The findings of the present study showed that single-phase FA episodes reduce survival and induce oxidative stress in S. hasta juveniles; meanwhile A. latus juveniles did not show complete compensatory growth after RF episode.

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“…The study has shown that the PK relative expression level of European seabass Dicentrarchus labrax in fasting for 21 days promotes a drastic 45-fold down-regulation in relation to the level in the fed seabass, which is similar to our research results. (Viegas et al, 2014). In this study (Fig.…”

Section: Discussionmentioning

confidence: 50%

Enzyme activities and gene expression in digestion and metabolism of Hyriopsis cumingii under different temperature and diet ration

Sun

Liu

et al. 2022

Preprint

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The traditional culture method of Hyriopsis cumingii is in urgent need of transformation, industrial aquaculture is a sustainable cultivation mode, and the control of environmental conditions and food is the basis and key. To evaluate combined effects of rations (high-ration, medium-ration, and low-ration) and temperatures (13 ± 0.2℃, 23 ± 0.2℃ and 33 ± 0.2℃) on digestion and metabolism of H. cumingii, a 3 × 3 factor comprehensive test was established. Levels of digestive enzymes like amylase (AMS), lipase (LPS), cellulase (CL), metabolic enzymes like glutamic pyruvate transaminase (GPT), glutamic oxaloacetate transaminase (GOT), pyruvate kinase (PK), hexokinase (HK), and metabolism-related genes like carnitine palmitoyltransferase-1 (CPT1), low-density lipoprotein receptor (LDLR) of mussels were analyzed during 21 days. Results showed levels of digestive and metabolic indicators remained stable in the medium-ration × 23℃ treatment, and were significantly (P < 0.05) higher than others on day 21. When exposed to low-ration, high-ration, 13℃ or 33℃, these biochemical indicators rose briefly and then significantly (P < 0.05) declined. Results suggested the medium-ration and 23℃ were the best diet quantity and temperature for H. cumingii. Short-term thermal, cold, semi-starving, and overfeeding treatments induced metabolic levels of mussels to meet the energy demand of anti-oxidative stress. However, long-term stress resulted in liver injury of H. cumingii, reduced the digestive and metabolic efficiency of the mussel, and affected its growth performance and health. This study has practical significance in exploring culture environment and feeding conditions, controlling breeding cost, and increasing the growth and yield in industrial aquaculture.

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Expressional regulation of key hepatic enzymes of intermediary metabolism in European seabass (Dicentrarchus labrax) during food deprivation and refeeding

Cited by 7 publications

References 44 publications

Contribution of dietary starch to hepatic and systemic carbohydrate fluxes in European seabass (Dicentrarchus labraxL.)

Contribution of dietary starch to hepatic and systemic carbohydrate fluxes in European seabass (Dicentrarchus labraxL.)

Effects of a single-phase fasting period and subsequent re-feeding on compensatory growth, digestive enzyme activities, and antioxidant capacity of sobaity (Sparidentex hasta) and yellowfin seabream (Acanthopagrus latus)

Enzyme activities and gene expression in digestion and metabolism of Hyriopsis cumingii under different temperature and diet ration

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