LeishMan Recommendations for Treatment of Cutaneous and Mucosal Leishmaniasis in Travelers, 2014

Blum, Johannes; Buffet, Pierre; Visser, Leo G.; Harms, Gundel; Bailey, Margaret; Caumes, Éric; Clerinx, Jan; Thiel, Pieter P. A. M. van; Morizot, Gloria; Hatz, Christoph; Dorlo, Thomas P. C.; Lockwood, Diana N J

doi:10.1111/jtm.12089

Cited by 117 publications

(108 citation statements)

References 134 publications

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“…This was also concluded in several meta-analyses of clinical trials of CL and MCL (19,246). For patient management, 64% of studies indicated the use of species identification methods, even though in such cases it is also considered relevant (16,247).…”

Section: Methods In Different Study Typesmentioning

confidence: 99%

“…A variety of methods were used. In clinical practice, these countries deal with import leishmaniasis (15,16,248), and as the site of infection in such cases is not always known, globally applicable typing strategies are needed. Neither minicircles nor rDNA provides such a possibility, so other methods must be sought.…”

Section: Methods In Different Geographical Areasmentioning

confidence: 99%

“…Verification of the Leishmania species is considered a must in any report describing clinical data and is a valuable aid in day-to-day patient management (16,19,76,(245)(246)(247). To this end, singlegene sequencing is the most practical method in a clinical (human or animal) context.…”

Section: Recommendationsmentioning

confidence: 99%

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Species Typing in Dermal Leishmaniasis

2015

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SUMMARY Leishmania is an infectious protozoan parasite related to African and American trypanosomes. All Leishmania species that are pathogenic to humans can cause dermal disease. When one is confronted with cutaneous leishmaniasis, identification of the causative species is relevant in both clinical and epidemiological studies, case management, and control. This review gives an overview of the currently existing and most used assays for species discrimination, with a critical appraisal of the limitations of each technique. The consensus taxonomy for the genus is outlined, including debatable species designations. Finally, a numerical literature analysis is presented that describes which methods are most used in various countries and regions in the world, and for which purposes.

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Section: Methods In Different Study Typesmentioning

confidence: 99%

Section: Methods In Different Geographical Areasmentioning

confidence: 99%

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Species Typing in Dermal Leishmaniasis

2015

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“…Side effects of antimonial treatment include nausea, vomiting, diarrhea, fatigue, pancreatitis, cytopenias, and reversible electrocardiogram (ECG) changes. Cure rates can range widely from 52 to 95 % with antimonials [47][48][49]. Oral miltefosine may also be used in both Old World and New World disease as it has been shown to be efficacious and relatively well tolerated compared with antimonials [50].…”

Section: Treatmentmentioning

confidence: 99%

Got the Travel Bug? A Review of Common Infections, Infestations, Bites, and Stings Among Returning Travelers

2016

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The popularity of international travel continues to increase among Americans, even though they often experience subsequent illness on return from their journey. The pathogens responsible are not necessarily endemic to the destination itself but are often the result of poor sanitary conditions or activities engaged in while away. Skin disease ranks third among all medical concerns in returning travelers. This review addresses the pathogenesis, epidemiology, clinical presentation, and treatment of the most common skin diseases in returning travelers: insect bites and bedbugs, cutaneous larva migrans, scabies, tungiasis, myiasis, leishmaniasis, viral exanthems, and marine envenomation. Primary care physicians and dermatologists should be familiar with these illnesses and a general approach to their evaluation and management. Key PointsArthropod assault and infestation is a source of significant morbidity in returning travelers.Travelers should bring proper protective clothing and insect repellant when travelling to areas where biting insects are vectors of disease.Travelers should be acquainted with the hazards associated with local marine life if they plan on entering the water.

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“…in the subgenera Viannia and Leishmania; Ͼ1 species may be found in the same geographic region. Species identification often has clinical relevance, such as implications regarding whether and which treatment is indicated and whether and how to monitor for potential sequelae of the infection (e.g., mucosal leishmaniasis, which is typically caused by New World species in the Viannia subgenus, particularly, but not only, by Leishmania [Viannia] braziliensis in certain geographic regions) (1,(3)(4)(5)(6)(7).…”

mentioning

confidence: 99%

Detection and Differentiation of Leishmania spp. in Clinical Specimens by Use of a SYBR Green-Based Real-Time PCR Assay

et al. 2017

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Leishmaniasis in humans is caused by Leishmania spp. in the subgenera Leishmania and Viannia. Species identification often has clinical relevance. Until recently, our laboratory relied on conventional PCR amplification of the internal transcribed spacer 2 (ITS2) region (ITS2-PCR) followed by sequencing analysis of the PCR product to differentiate Leishmania spp. Here we describe a novel real-time quantitative PCR (qPCR) approach based on the SYBR green technology (LSG-qPCR), which uses genus-specific primers that target the ITS1 region and amplify DNA from at least 10 Leishmania spp., followed by analysis of the melting temperature (T m ) of the amplicons on qPCR platforms (the Mx3000P qPCR system [Stratagene-Agilent] and the 7500 real-time PCR system [ABI Life Technologies]). We initially evaluated the assay by testing reference Leishmania isolates and comparing the results with those from the conventional ITS2-PCR approach. Then we compared the results from the real-time and conventional molecular approaches for clinical specimens from 1,051 patients submitted to the reference laboratory of the Centers for Disease Control and Prevention for Leishmania diagnostic testing. Specimens from 477 patients tested positive for Leishmania spp. with the LSG-qPCR assay, specimens from 465 of these 477 patients also tested positive with the conventional ITS2-PCR approach, and specimens from 10 of these 465 patients had positive results because of retesting prompted by LSG-qPCR positivity. On the basis of the T m values of the LSG-qPCR amplicons from reference and clinical specimens, we were able to differentiate four groups of Leishmania parasites: the Viannia subgenus in aggregate; the Leishmania

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LeishMan Recommendations for Treatment of Cutaneous and Mucosal Leishmaniasis in Travelers, 2014

Abstract: In this article, the authors provide practical treatment recommendations for imported CL and ML in Europe, drawn up from the review by the European experts.

Cited by 117 publications

References 134 publications

Species Typing in Dermal Leishmaniasis

Species Typing in Dermal Leishmaniasis

Got the Travel Bug? A Review of Common Infections, Infestations, Bites, and Stings Among Returning Travelers

Detection and Differentiation of Leishmania spp. in Clinical Specimens by Use of a SYBR Green-Based Real-Time PCR Assay

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