Presencia del genotipo Beijing entre cepas del complejo Mycobacterium tuberculosis en dos centros de salud de la Región Metropolitana-Chile

Meza, Paulina; Balcells, María Elvira; Miranda, Carolina; Cifuentes, Marcela; Wozniak, Aniela; García, Patricia

doi:10.4067/s0716-10182014000100003

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…However, it might have remained undetected due to limitations in the study's sample representativeness. Subsequent nation-wide studies of M. tuberculosis lineages demonstrated the Beijing lineage in Colombia (10), where the first case of multidrug-resistant tuberculosis by a rare Beijing strain was reported, and more recently, in Chile (9,11), where Beijing strains were found in isolates from Santiago.…”

Section: Discussionmentioning

confidence: 99%

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Identification of the Mycobacterium tuberculosis Beijing lineage in Ecuador

et al. 2017

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Introducción. Los aislados de Mycobacterium tuberculosis pertenecientes al linaje Beijing son considerados especialmente virulentos, transmisibles y con mayor tendencia a la adquisición de resistencias. El linaje Beijing ha sido descrito en todo el mundo, sin embargo estudios al respecto son más escasos en Latinoamérica. El único estudio multinacional realizado en la region detectó una distribución heterogénea de este linaje, estando ausente en Chile, Colombia y Ecuador. Estudios nacionales posteriores ya identificaron aislados Beijing en Chile y Colombia.Objetivo. Analizar la presencia del linaje Beijing de M. tuberculosis en Ecuador, el único país en la región en el que aún permanece inédito.Materiales y métodos. Se analizó una muestra de conveniencia (2006-2012), obtenida de dos hospitales que cubrían poblaciones diferentes. El genotipado de los aislados de M. tuberculosis se realizó por 24-MIRU-VNTR. La asignación de linajes se realizó mediante comparación de los patrones genotípicos con los incluidos en la plataforma MIRU-VNTRplus y aquellos pertenecientes al linaje Beijing fueron confirmados mediante PCR alelo-específica.Resultados. Se identificó el primer aislado Beijing descrito en Ecuador, en un escenario epidemiológico inesperado, infectando a un paciente de la región andina, en una comunidad con escasa movilidad y alejada de las fronteras con los países limítrofes, Perú y Colombia, en los que ya se han identificado aislados de M. tuberculosis pertenecientes al linaje Beijing.Conclusiones. Este trabajo constituye la primera descripción de la presencia del linaje Beijing de M. tuberculosis en Ecuador, y sugiere un contexto epidemiológico singular que merece especial atención.

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Section: Discussionmentioning

confidence: 99%

“…In the remaining three countries (Chile, Colombia and Ecuador) no Beijing isolates were identified. Subsequent nationwide studies, however, found Beijing lineage isolates in Colombia and Chile (9)(10)(11). Ecuador is the only country among those considered in the study where this lineage had not been reported yet.…”

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confidence: 82%

Identification of the Mycobacterium tuberculosis Beijing lineage in Ecuador

et al. 2017

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“…In this context, the introduction of molecular epidemiology tools is a valuable asset to help understand local TB transmission dynamics. Two previous reports published locally have described genotyping of a small number of MTBC strains collected at the X th region in year 2006 and Metropolitan Region in 2013, and the most frequently identified spoligotypes were T1 (24%) and LAM (39.5%), respectively [ 10 , 11 ]. However, none of those reports included phylogenetic analysis or epidemiological data to distinguish local versus imported strains.…”

Section: Introductionmentioning

confidence: 99%

A First Insight on the Population Structure of Mycobacterium tuberculosis Complex as Studied by Spoligotyping and MIRU-VNTRs in Santiago, Chile

et al. 2015

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Tuberculosis (TB) remains a significant public health problem worldwide, but the ecology of the prevalent mycobacterial strains, and their transmission, can vary depending on country and region. Chile is a country with low incidence of TB, that has a geographically isolated location in relation to the rest of South American countries due to the Andes Mountains, but recent migration from neighboring countries has changed this situation. We aimed to assess the genotypic diversity of Mycobacterium tuberculosis complex (MTBC) strains in Santiago, Chile, and compare with reports from other Latin-American countries. We analyzed MTBC isolates from pulmonary tuberculosis cases collected between years 2008 and 2013 in Central Santiago, using two genotyping methods: spoligotyping and 12-loci mycobacterial interspersed repetitive unit-variable number of tandem repeats (MIRU-VNTRs). Data obtained were analyzed and compared to the SITVIT2 database. Mean age of the patients was 47.5 years and 61% were male; 11.6% were migrants. Of 103 strains (1 isolate/patient) included, there were 56 distinct spoligotype patterns. Of these, 16 strains (15.5%) corresponded to orphan strains in the SITVIT2 database, not previously reported. Latin American and Mediterranean (LAM) (34%) and T (33%) lineages were the most prevalent strains, followed by Haarlem lineage (16.5%). Beijing family was scarcely represented with only two cases (1.9%), one of them isolated from a Peruvian migrant. The most frequent clustered spoligotypes were SIT33/LAM3 (10.7%), SIT53/T1 (8.7%), SIT50/H3 (7.8%), and SIT37/T3 (6.8%). We conclude that LAM and T genotypes are the most prevalent genotypes of MTBC in Santiago, Chile, and together correspond to almost two thirds of analyzed strains, which is similar to strain distribution reported from other countries of Latin America. Nevertheless, the high proportion of SIT37/T3, which was rarely found in other Latin American countries, may underline a specific history or demographics of Chile related to probable human migrations and evolutions.

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“…Various phylogenetic lineages of the Mtb complex are distributed differently around the world [ 2 ]. In Latin America, both drug susceptible and drug resistant TB are mainly related to the Euro–American Lineage [ 26 , 27 , 28 , 29 ] and the Beijing strain has a minor impact, in contrast to what is reported in other regions. Drug resistance databases mostly rely on the genome H37Rv strain.…”

Section: Discussionmentioning

confidence: 99%

Genetic Identification and Drug-Resistance Characterization of Mycobacterium tuberculosis Using a Portable Sequencing Device. A Pilot Study

2020

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Clinical management of tuberculosis (TB) in endemic areas is often challenged by a lack of resources including laboratories for Mycobacterium tuberculosis (Mtb) culture. Traditional phenotypic drug susceptibility testing for Mtb is costly and time consuming, while PCR-based methods are limited to selected target loci. We herein utilized a portable, USB-powered, long-read sequencing instrument (MinION), to investigate Mtb genomic DNA from clinical isolates to determine the presence of anti-TB drug-resistance conferring mutations. Data analysis platform EPI2ME and antibiotic-resistance analysis using the real time ARMA workflow, identified Mtb species as well as extensive resistance gene profiles. The approach was highly sensitive, being able to detect almost all described drug resistance conferring mutations based on previous whole genome sequencing analysis. Our findings are supportive of the practical use of this system as a suitable method for the detection of antimicrobial resistance genes, and effective in providing Mtb genomic information. Future improvements in the error rate through statistical analysis, drug resistance prediction algorithms and reference databases would make this a platform suited for the clinical setting. The small size, relatively inexpensive cost of the device, as well as its rapid and simple library preparation protocol and analysis, make it an attractive option for settings with limited laboratory infrastructure.

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Presencia del genotipo Beijing entre cepas del complejo Mycobacterium tuberculosis en dos centros de salud de la Región Metropolitana-Chile

Cited by 10 publications

References 16 publications

Identification of the Mycobacterium tuberculosis Beijing lineage in Ecuador

Identification of the Mycobacterium tuberculosis Beijing lineage in Ecuador

A First Insight on the Population Structure of Mycobacterium tuberculosis Complex as Studied by Spoligotyping and MIRU-VNTRs in Santiago, Chile

Genetic Identification and Drug-Resistance Characterization of Mycobacterium tuberculosis Using a Portable Sequencing Device. A Pilot Study

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