Prevalence and molecular identification of Chlamydia abortus in commercial dairy goat farms in a hot region in Mexico

Campos-Hernández, Eleuterio; Vázquez-Chagoyán, Juan Carlos; Salem, Abdelfattah Z.M.; Saltijeral-Oaxaca, J. A.; Escalante-Ochoa, Cristina; López-Heydeck, Sandra M.; Jiménez, Roberto Montes de Oca

doi:10.1007/s11250-014-0585-6

Cited by 31 publications

(23 citation statements)

References 28 publications

(38 reference statements)

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“…Chlamydia has been detected in the GI tracts of animals [ 1 ] and humans [ 2 – 5 ]. However, the medical significance of the chlamydial infection in the GI tract remains unclear.…”

Section: Discussionmentioning

confidence: 99%

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The Chlamydia muridarum Organisms Fail to Auto-Inoculate the Mouse Genital Tract after Colonization in the Gastrointestinal Tract for 70 days

et al. 2016

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Chlamydia muridarum is known to colonize in the gastrointestinal tract for long periods of time, which has been hypothesized to serve as a reservoir for spreading to the genital tract. To test this hypothesis, a luciferase-expressing C. muridarum was used to establish a long-lasting infection in the mouse gastrointestinal tract following either intragastric or intrarectal inoculations. In vivo imaging revealed significant bioluminescent signals mainly in the mouse abdominal area throughout the experiments. Ex vivo imaging localized the signals to the mouse gastrointestinal tract, which was confirmed by monitoring the C. muridarum organisms in the mouse organs/tissues. Despite the long-lasting colonization in the gastrointestinal tract and active shedding of infectious organisms in the rectal swabs, the organisms did not cause any significant infection or pathology in the genital tract throughout the experiments, which was reproduced in multiple strains of mice and with an increased inoculation dose to the gastrointestinal tract. The above observations have demonstrated that the long-lasting C. muridarum organisms from the gastrointestinal tract are inefficient in auto-inoculating the genital tract, suggesting that the gastrointestinal tract Chlamydia may utilize an indirect mechanism to affect its pathogenicity in the genital tract.

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“…Chlamydia has been detected in the GI tracts of animals [ 1 ] and humans [ 2 – 5 ]. However, the medical significance of the chlamydial infection in the GI tract remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Chlamydia has been detected in the gastrointestinal (GI) tracts of animals [ 1 ] and humans [ 2 – 5 ]. Chlamydia muridarum is known to last for long periods of time in the mouse GI tract [ 6 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Chlamydia muridarum Organisms Fail to Auto-Inoculate the Mouse Genital Tract after Colonization in the Gastrointestinal Tract for 70 days

et al. 2016

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“…Additionally, C. abortus has also been reported as a zoonotic pathogen because it can also result in abortion in humans (Psarrakos et al 2011;Campos-Hernández et al 2014). Moreover, other human health disorders including sepsis during pregnancy and pelvic inflammatory disease have been considered to be associated with C. abortus infection (Rodolakis and Yousef Mohamad 2010;Wheelhouse and Longbottom 2012).…”

Section: Introductionmentioning

confidence: 99%

Herd-level prevalence and associated risk factors for Toxoplasma gondii, Neospora caninum, Chlamydia abortus and bovine viral diarrhoea virus in commercial dairy and beef cattle in eastern, northern and northeastern China

et al. 2015

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Although the seroprevalence of Toxoplasma gondii, Neospora caninum, Chlamydia abortus and bovine viral diarrhea virus infection in cattle have been reported in some areas in China, most of them were conducted with small number of cattle samples and very limited districts and neglected the assessment of herd management factors associated with herd-level prevalence of these pathogen infections. Thus, from September 2013 to December 2014, a large-scale seroprevalence study was conducted to determine the animal-level and herd-level seroprevalence and identify herd-level risk factors associated with these pathogen infections in 4487 cattle from 134 herds in five provinces (Heilongjiang, Jilin, Liaoning, Shandong, Hebei) and Inner Mongolia Autonomous Region of China. At animal level, the true prevalence of antibodies against T. gondii, N. caninum, C. abortus and bovine viral diarrhoea virus (BVDV) was 10.48, 17.14, 11.92 and 50.10%, respectively. At herd level, the true prevalence of antibodies against T. gondii, N. caninum, C. abortus and BVDV was 27.16, 29.10, 37.31 and 40.30%, respectively. Multivariate analysis of these characteristics showed that source of water and presence of felids were significantly associated with T. gondii infection in the studied cattle herds. Source of water was significantly associated with N. caninum infection in the studied cattle herds. While herd size and management system were significantly associated with BVDV infection in the studied cattle herds, this is the first report of herd-level prevalence and associated risk factors of T. gondii, N. caninum, C. abortus and BVDV infection in cattle in China.

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“…Large-scale screenings of both cervicovaginal and rectal swabs not only will provide information for treatment and follow-up but may also aid in our understanding of the exact relationships between the vaginal and rectal C. trachomatis organisms in humans. The frequent detection of Chlamydia abortus in multiple organs of large animals (31) indicates that the C. abortus organisms may also be able to spread systemically in large animals just as the C. muridarum organisms do in mice.…”

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confidence: 99%

In VivoandEx VivoImaging Reveals a Long-Lasting Chlamydial Infection in the Mouse Gastrointestinal Tract following Genital Tract Inoculation

et al. 2015

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Intravaginal infection with Chlamydia muridarum in mice can ascend to the upper genital tract, resulting in hydrosalpinx, a pathological hallmark for tubal infertility in women infected with C. trachomatis. Here, we utilized in vivo imaging of C. muridarum infection in mice following an intravaginal inoculation and confirmed the rapid ascent of the chlamydial organisms from the lower to upper genital tracts. Unexpectedly, the C. muridarum-derived signal was still detectable in the abdominal area 100 days after inoculation. Ex vivo imaging of the mouse organs revealed that the long-lasting presence of the chlamydial signal was restricted to the gastrointestinal (GI) tract, which was validated by directly measuring the chlamydial live organisms and genomes in the same organs. The C. muridarum organisms spreading from the genital to the GI tracts were detected in different mouse strains and appeared to be independent of oral or rectal routes. Mice prevented from orally taking up excretions also developed the long-lasting GI tract infection. Inoculation of C. muridarum directly into the upper genital tract, which resulted in a delayed vaginal shedding of live organisms, accelerated the chlamydial spreading to the GI tract. Thus, we have demonstrated that the genital tract chlamydial organisms may use a systemic route to spread to and establish a long-lasting infection in the GI tract. The significance of the chlamydial spreading from the genital to GI tracts is discussed.

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Prevalence and molecular identification of Chlamydia abortus in commercial dairy goat farms in a hot region in Mexico

Cited by 31 publications

References 28 publications

The Chlamydia muridarum Organisms Fail to Auto-Inoculate the Mouse Genital Tract after Colonization in the Gastrointestinal Tract for 70 days

The Chlamydia muridarum Organisms Fail to Auto-Inoculate the Mouse Genital Tract after Colonization in the Gastrointestinal Tract for 70 days

Herd-level prevalence and associated risk factors for Toxoplasma gondii, Neospora caninum, Chlamydia abortus and bovine viral diarrhoea virus in commercial dairy and beef cattle in eastern, northern and northeastern China

In VivoandEx VivoImaging Reveals a Long-Lasting Chlamydial Infection in the Mouse Gastrointestinal Tract following Genital Tract Inoculation

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