Lithium carbonate teratogenic effects in chick cardiomyocyte micromass system and mouse embryonic stem cell derived cardiomyocyte – Possible protective role of myo-inositol

Qureshi, Wasay Mohiuddin Shaikh; Latif, M.L.; Parker, Terry L.; Pratten, Margaret K.

doi:10.1016/j.reprotox.2014.03.009

Cited by 10 publications

(3 citation statements)

References 45 publications

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“…We then tested other category D dugs with various therapeutic applications. Lithium carbonate (category D antidepressant), which inhibits the PKC signaling for its psychiatric medication purpose, appeared to inhibit phosphatidylinositol cycle and Wnt pathway activation, based on mEST assays ( Shaikh Qureshi et al., 2014 ). Exposure of lithium did not affect the contractile functions of the cardiac organoids but exhibited mild toxicity to the organoid formation measured by area ratio and FWHM ( Figure S5 B).…”

Section: Resultsmentioning

confidence: 99%

“…Especially, category D drugs (phenytoin, lithium, doxycycline, and tretinoin) showed diverse effects on developmental toxicity. Surprisingly, exposure of lithium only showed mild developmental toxicity of slight reduction in cardiac differentiation and organoid formation, although there has been a long debate of this drugs' developmental toxicity, especially resulting in congenital heart defects ( Hoberman et al., 1990 ; Shaikh Qureshi et al., 2014 ).…”

Section: Discussionmentioning

confidence: 99%

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Engineering spatial-organized cardiac organoids for developmental toxicity testing

et al. 2021

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Summary Emerging technologies in stem cell engineering have produced sophisticated organoid platforms by controlling stem cell fate via biomaterial instructive cues. By micropatterning and differentiating human induced pluripotent stem cells (hiPSCs), we have engineered spatially organized cardiac organoids with contracting cardiomyocytes in the center surrounded by stromal cells distributed along the pattern perimeter. We investigated how geometric confinement directed the structural morphology and contractile functions of the cardiac organoids and tailored the pattern geometry to optimize organoid production. Using modern data-mining techniques, we found that pattern sizes significantly affected contraction functions, particularly in the parameters related to contraction duration and diastolic functions. We applied cardiac organoids generated from 600 μm diameter circles as a developmental toxicity screening assay and quantified the embryotoxic potential of nine pharmaceutical compounds. These cardiac organoids have potential use as an in vitro platform for studying organoid structure-function relationships, developmental processes, and drug-induced cardiac developmental toxicity.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Engineering spatial-organized cardiac organoids for developmental toxicity testing

et al. 2021

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“…The most effective in vitro method for differentiation into cardiac muscle cells is by mimicking in vivo pathways that regulate the establishment of cardiac lineage during early development [ 65 ]. During early stages of cardiac differentiation, the cardiac mesoderm, can be induced by the temporal expression of KDR (Flk-1) [ 66 ].…”

Section: Ipsc Differentiation Into Cardiomyocytesmentioning

confidence: 99%

Induced Pluripotent Stem Cells and Their Use in Cardiac and Neural Regenerative Medicine

Skalová

Švadláková

Qureshi

et al. 2015

IJMS

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Stem cells are unique pools of cells that are crucial for embryonic development and maintenance of adult tissue homeostasis. The landmark Nobel Prize winning research by Yamanaka and colleagues to induce pluripotency in somatic cells has reshaped the field of stem cell research. The complications related to the usage of pluripotent embryonic stem cells (ESCs) in human medicine, particularly ESC isolation and histoincompatibility were bypassed with induced pluripotent stem cell (iPSC) technology. The human iPSCs can be used for studying embryogenesis, disease modeling, drug testing and regenerative medicine. iPSCs can be diverted to different cell lineages using small molecules and growth factors. In this review we have focused on iPSC differentiation towards cardiac and neuronal lineages. Moreover, we deal with the use of iPSCs in regenerative medicine and modeling diseases like myocardial infarction, Timothy syndrome, dilated cardiomyopathy, Parkinson’s, Alzheimer’s and Huntington’s disease. Despite the promising potential of iPSCs, genome contamination and low efficacy of cell reprogramming remain significant challenges.

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Evaluation of Bupropion Hydrochloride Developmental Cardiotoxic Effects in Chick Cardiomyocyte Micromass Culture and stem cell derived Cardiomyocyte Systems

Qureshi

Latif

Parker

et al. 2014

Birth Defects Research Pt B

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The use of antidepressant drug bupropion hydrochloride (BPN) during pregnancy results in increased cardiovascular anomalies. In this study, BPN developmental cardiotoxic effects in in vitro system were evaluated using chick cardiomyocyte micromass (MM) culture system and mouse embryonic stem cell derived cardiomyocyte (ESDC) system. In MM system, the cardiomyocyte contractile activity significantly decreased only at BPN 200 μM, while in ESDC system BPN concentration above 75 μM resulted in decreased contractile activity. The increase in drug concentration also affected the cardiomyocyte viability and total cellular protein content in both systems, but in ESDC system the cell viability failed to attain significant difference. The drug failed to induce reactive oxygen species production in both systems, but has affected the cardiac connexin43 expression especially in MM system. We observed that BPN showed developmental cardiotoxic effects irrespective of the stage of cardiac development in both in vitro systems.

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Lithium carbonate teratogenic effects in chick cardiomyocyte micromass system and mouse embryonic stem cell derived cardiomyocyte – Possible protective role of myo-inositol

Cited by 10 publications

References 45 publications

Engineering spatial-organized cardiac organoids for developmental toxicity testing

Engineering spatial-organized cardiac organoids for developmental toxicity testing

Induced Pluripotent Stem Cells and Their Use in Cardiac and Neural Regenerative Medicine

Evaluation of Bupropion Hydrochloride Developmental Cardiotoxic Effects in Chick Cardiomyocyte Micromass Culture and stem cell derived Cardiomyocyte Systems

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