Fabrication of Multiresponsive Bioactive Nanocapsules through Orthogonal Self‐Assembly

Abstract

Multifunctional self-assembled systems present platforms for fundamental research and practical applications, providing tunability of structure, functionality and stimuli response. Pragmatic structures for biological applications have multiple design requirements, including control of size, stability and environmental response. Here we present the fabrication of multifunctional nanoparticle-stabilized capsules (NPSCs) using a set of orthogonal supramolecular interactions. In these capsules, fluorescent proteins are attached to quantum dots through polyhistidine coordination. These anionic assemblies interact laterally with cationic gold nanoparticles that are anchored to the fatty acid core through guanidinium-carboxylate interactions. The lipophilic core then provides a reservoir for hydrophobic endosome-disrupting agents, generating a system featuring stimuli-responsive payload release into the cytosol with fluorescent monitoring.

Fabrication of Functional Nanofibers Through Post‐Nanoparticle Functionalization

“…Beyond the properties provided by the particles, these functionalized materials provide scaffolds for further supramolecular functionalization. Supra­molecular assembly has been used to generate nanocomposites for protein delivery, gene delivery, and sensing, demonstrating the biofriendly nature of the strategy. CdSe/ZnS core–shell QDs ( λ max = 555 nm) featuring TTMA functionality (Scheme ) were synthesized as previously reported .…”

Rational Design of Nanocarriers for Intracellular Protein Delivery

“…Furthermore, epidermal growth factor (EGF), a target protein could be loaded onto the AuNP-(His-GST)-aptamer system with BIM protein together, showing significantly uptake in mice and growth inhibition of xenograft tumors without detectable systemic toxicity. [161,162] The AuNPs were first bonded with a tetrapeptide, His-Lys-Arg-Lys (HKRK), which interacted with carboxylates of the oil to pin the AuNPs on the capsule surface. [159] The DNA layer provided terminal amine groups used to conjugate the Ni 2+ and protein chelator.…”

Inorganic/Organic Multilayer Capsule Composition for Improved Functionality and External Triggering

“…[52,53] Because usually situation is complicated for many practical applications, for example, different environmental changes may occur at the same time, where multifunctional materials are required to overcome challenges that cannot be solved by the individual components. [52,53] Because usually situation is complicated for many practical applications, for example, different environmental changes may occur at the same time, where multifunctional materials are required to overcome challenges that cannot be solved by the individual components.…”