In situ antimicrobial activity on oral biofilm: essential oils vs. 0.2 % chlorhexidine

Quintas, Victor Gandra; Prada-López, Isabel; Prados-Frutos, Juan Carlos; Tomás, Inmaculada

doi:10.1007/s00784-014-1224-3

Cited by 32 publications

(61 citation statements)

References 40 publications

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“…8 The promising potential of natural substances against cariogenic microorganisms, and their application in the development of preventive and therapeutic products, has been explored in recent years. 9,10 There are already some commercially available formulations of mouthwashes and dentifrices containing natural substances. 11,12 Previous studies have reported the effects of natural substances on microbial growth, polysaccharides synthesis and dental biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

Oliveira¹,

Borges²,

Brighenti

et al. 2017

Braz. oral res.

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The objective of this study was to evaluate the effects of Cymbopogon citratus essential oil and its main compound (citral) against primary dental colonizers and caries-related species. Chemical characterization of the essential oil was performed by gas chromatography/mass spectroscopy (GC/MS), and the main compound was determined. Antimicrobial activity was tested against Actinomyces naeslundii, Lactobacillus acidophilus, S. gordonii, S. mitis, S. mutans, S. sanguinis and S. sobrinus. Minimum inhibitory and bactericide concentrations were determined by broth microdilution assay for streptococci and lactobacilli reference, and for clinical strains. The effect of the essential oil on bacterial adhesion and biofilm formation/disruption was investigated. Negative (without treatment) and positive controls (chlorhexidine) were used. The effect of citral on preformed biofilm was also tested using the same methodology. Monospecies and microcosm biofilms were tested. ANOVA or Kruskal-Wallis tests were used (α=0.05). Cytotoxicity of the essential oil to human keratinocytes was performed by MTT assay. GC/MS demonstrated one major component (citral). The essential oil showed an inhibitory effect on all tested bacterial species, including S. mutans and L. acidophilus. Essential oil of C. citratus (10X MIC) reduced the number of viable cells of lactobacilli and streptococci biofilms (p < 0.05). The essential oil inhibited adhesion of caries-related polymicrobial biofilm to dental enamel (p < 0.01). Citral significantly reduced the number of viable cells of streptococci biofilm (p < 0.001). The essential oil showed low cytotoxicity to human keratinocytes. Based on these findings, this study can contribute to the development of new formulations for products like mouthwash, against dental biofilms.

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Section: Introductionmentioning

confidence: 99%

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

Oliveira¹,

Borges²,

Brighenti

et al. 2017

Braz. oral res.

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show abstract

“…On the other hand, in vivo models vary from methods based on plaque re-growth assessments on short periods of time 17 or others using saliva [8][9][10]12,18,19 and/or dental plaque after a single application. [19][20][21] Fig. 1 -Scheme of the protocol followed by all volunteers from the randomization to the application of the different tests.…”

Section: Introductionmentioning

confidence: 99%

In situ neutralisation of the antibacterial effect of 0.2% Chlorhexidine on salivary microbiota: Quantification of substantivity

Quintas

Prada-López

Donos

et al. 2015

Archives of Oral Biology

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“…The use of this toothbrush rinse was also evaluated as an alternative treatment to disinfect toothbrushes. Several studies have evaluated substances that could be used as disinfectants for toothbrushes, seeking an easy, economical and safe use for disinfecting them and thereby maintaining oral health (Quintas et al, 2015;Nelson-Filho et al, 2011;Zarai et al, 2011;Juiz et al, 2010;Efstratiou et al, 2007;Mehta et al, 2007). The present study showed the in vitro and ex vivo effects of P. heptaphyllum mouthrinse against the gram positive bacteria group MS.…”

Section: Discussionmentioning

confidence: 99%

Stability and disinfecting proprieties of the toothbrush rinse of the essential oil of Protium heptaphyllum

Fernanda¹,

Otavio²,

Mirian³

et al. 2015

Afr. J. Pharm. Pharmacol.

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Protium heptaphyllum (PH) is rich in essential oil, has anti-inflammatory properties and has no toxic potential. However, data is unavailable about its antiseptic effect against bacteria that cause caries. This study aimed to evaluate the antiseptic effect of the toothbrush rinse of essential oil of PH as well as its chemical stability. The toothbrush rinse was prepared with 1% essential oil of PH. The minimum inhibitory concentration (MIC) of the essential oil and toothbrush rinse were evaluated against Streptococcus mutans (ATCC 25175™). The ex vivo study was double-blinded and randomised; the children were divided into three groups, each participating in a crossover design where all solutions (water, toothbrush rinse (1%) and chlorhexidine (0.12%) were used in all stages by different groups of children. The chemical composition of the essential oil and toothbrush rinse were analysed by gas chromatography coupled to mass spectrometry. The stability was evaluated at three time points. The essential oil and toothbrush rinse exhibited antimicrobial activity against S. mutans, MIC = 0.125 and 2.4 µg/ml. The toothbrush rinse showed the same effect as chlorhexidine on disinfecting the toothbrushes contaminated with mutans streptococci (pH = 57.3 ± 5.3%; chlorhexidine 55.5 ± 13.3%; water 39.4 ± 5.8%; p > 0.05). Chromatographic analysis showed that the essential oil contained monoterpenes as a major component, and the toothbrush rinse possessed the same constituents as the pure essential oil, except for α-terpineol. Storage did not cause chemical degradation of the toothbrush rinse, but decreased the concentration of the chemical constituents. The toothbrush rinse of essential oil of P. heptaphyllum showed antiseptic properties and exhibited antimicrobial activity against mutans streptococci.

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In situ antimicrobial activity on oral biofilm: essential oils vs. 0.2 % chlorhexidine

Abstract: A single M-EO is an effective measure against the de novo biofilm, presenting a good alternative to clorhexidine such as a preoperative rinse, in periodontal procedures or post-treatment applications.

Cited by 32 publications

References 40 publications

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity

In situ neutralisation of the antibacterial effect of 0.2% Chlorhexidine on salivary microbiota: Quantification of substantivity

Stability and disinfecting proprieties of the toothbrush rinse of the essential oil of Protium heptaphyllum

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