Scaffold preferences of mesenchymal stromal cells and adipose-derived stem cells from green fluorescent protein transgenic mice influence the tissue engineering of bone

Wittenburg, Gretel; Flade, Viktoria; Garbe, Annette I.; Lauer, Günter; Labudde, Dirk

doi:10.1016/j.bjoms.2014.02.023

Cited by 9 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine if cells lose their dependence on FACT for viability upon differentiation, we isolated intestinal and adipose stem cells and grew them under conditions that either maintained their undifferentiated state or induced differentiation in vitro (Taha & Hedayati, 2010; Wittenburg et al , 2014; Lindeboom et al , 2018). For both types of cells, 4‐OHT administration was toxic only to undifferentiated cells.…”

Section: Resultsmentioning

confidence: 99%

FACT maintains nucleosomes during transcription and stem cell viability in adult mice

et al. 2022

View full text Add to dashboard Cite

Preservation of nucleosomes during replication has been extensively studied, while the maintenance of nucleosomes during transcription has gotten less attention. The histone chaperone FACT has a role in transcription elongation, although whether it disassembles or assembles nucleosomes during this process is unclear.To elucidate the function of FACT in mammals, we deleted the Ssrp1 subunit of FACT in adult mice. FACT loss is lethal, possibly due to the loss of the earliest progenitors in bone marrow and intestine, while more differentiated cells are not affected. Using cells isolated from several tissues, we show that FACT loss reduces the viability of stem cells but not of cells differentiated in vitro. FACT depletion increases chromatin accessibility in a transcriptiondependent manner in adipose mesenchymal stem cells, indicating that nucleosomes are lost in these cells during transcription in the absence of FACT. We also observe activation of interferon (IFN) signaling and the accumulation of immunocytes in organs sensitive to FACT loss. Our data indicate that FACT maintains chromatin integrity during transcription in mammalian adult stem cells, suggesting that chromatin transcription in stem cells and differentiated cells is different.

show abstract

Section: Resultsmentioning

confidence: 99%

FACT maintains nucleosomes during transcription and stem cell viability in adult mice

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Unchanged cellular phenotype is an important aspect of biocompatibility. The cells show scaffold preferences and, thus, the suitability of the material must be validated beforehand [42, 43]. The results of our study indicate that Hs27 cells grow well on both investigated surgical scaffolds and show apparently unchanged and typical fibroblast morphology.…”

Section: Discussionmentioning

confidence: 93%

Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for genetically-modified fibroblasts

et al. 2017

View full text Add to dashboard Cite

Recent advances in gene delivery into cells allow improved therapeutic effects in gene therapy trials. To increase the bioavailability of applied cells, it is of great interest that transfected cells remain at the application site and systemic spread is minimized. In this study, we tested clinically used biodegradable poly(lactic acid-co-glycolic acid) (PLGA) scaffolds (Vicryl & Ethisorb) as transient carriers for genetically modified cells. To this aim, we used human fibroblasts and examined attachment and proliferation of untransfected cells on the scaffolds in vitro, as well as the mechanical properties of the scaffolds at four time points (1, 3, 6 and 9 days) of cultivation. Furthermore, the adherence of cells transfected with green fluorescent protein (GFP) and vascular endothelial growth factor (VEGF165) and also VEGF165 protein secretion were investigated. Our results show that human fibroblasts adhere on both types of PLGA scaffolds. However, proliferation and transgene expression capacity were higher on Ethisorb scaffolds most probably due to a different architecture of the scaffold. Additionally, cultivation of the cells on the scaffolds did not alter their biomechanical properties. The results of this investigation could be potentially exploited in therapeutic regiments with areal delivery of transiently transfected cells and may open the way for a variety of applications of cell-based gene therapy, tissue engineering and regenerative medicine.

show abstract

“…To determine if cells lose their dependence on FACT for viability upon differentiation, we isolated intestinal and adipose stem cells and grew them under conditions that either maintained their undifferentiated state or induced differentiation in vitro [31][32][33]. For both types of cells, 4-OHT administration was toxic only to undifferentiated cells.…”

Section: Stem Cells From Several Organs Cannot Expand In Vitro In the Absence Of Factmentioning

confidence: 99%

“…Next, we tested if cells lose dependence on FACT for viability upon differentiation. For this, we isolated intestinal and adipose stem cells and kept them in conditions which either support their undifferentiated state or induce in vitro differentiation using protocols described in literature (33)(34)(35). In both cases, 4-OHT administration was toxic to undifferentiated cells, while has no effect on differentiated cells.…”

mentioning

confidence: 99%

FACT-mediated maintenance of chromatin integrity during transcription is essential for mammalian stem cell viability

Goswami

Sandlesh

Stablewski

et al. 2021

Preprint

View full text Add to dashboard Cite

Replication and transcription machineries access DNA through unwrapping it from histone core. Preservation of nucleosome structure during replication is a focus of intensive research, while maintenance of nucleosomes during transcription is less studied. Histone chaperone FACT is involved in transcription elongation, although whether it opens nucleosomes for polymerase or protects core histones from loss during passage of polymerase is still unclear. We used conditional knockout model of Ssrp1, subunit of FACT complex, to deplete FACT in mice and monitored consequences of FACT loss to establish the functional relevance of FACT in mammals. FACT loss was lethal for mice at all ages due to failure of hematopoietic and intestinal tissues. In these tissues, only the progenitors completely vanished upon FACT loss, while number of some other cell types were changed up and down. Using isolated stem cells of several tissues we showed that FACT loss was toxic only for stem cells, but not for cells which were differentiated in vitro. Chromatin accessibility in stem cells was increased genome wide upon FACT depletion in transcription dependent manner. The most prominent response to the loss of chromatin integrity upon FACT depletion in cells was activation of interferon signaling, followed by accumulation of immune cells in sensitive organs. Thus, in undifferentiated mammalian cells FACT keeps chromatin stable during transcription elongation and this function of FACT is essential for viability of stem cells.

show abstract

Scaffold preferences of mesenchymal stromal cells and adipose-derived stem cells from green fluorescent protein transgenic mice influence the tissue engineering of bone

Cited by 9 publications

References 12 publications

FACT maintains nucleosomes during transcription and stem cell viability in adult mice

FACT maintains nucleosomes during transcription and stem cell viability in adult mice

Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for genetically-modified fibroblasts

FACT-mediated maintenance of chromatin integrity during transcription is essential for mammalian stem cell viability

Contact Info

Product

Resources

About