Purification and Partial Characterization of an Acidic α-Amylase from a Newly IsolatedBacillus subtilisZJ-1 that may be Applied to Feed Enzyme

Abstract

An amylase-producing strain was isolated from soy sauce and designated as Bacillus subtilis ZJ-1. Purification of α-amylase from B. subtilis ZJ-1 to homogeneity by ethanol fractionation, ultrafiltration, and Sephadex G-100 gel filtration resulted in recovery of 8.9% and a specific activity of 542.7 U/mg protein. The molecular mass was estimated to be 58 kD by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme reached its maximum activity at a pH of 5.0 and a temperature of 50°C. The enzyme remained at 89.4 ± 3.0% of its activity at 40°C. The enzyme retained 87.7 ± 3.7% and 63.4 ± 2.9% of its original activity at 40°C after a 60-min incubation in the presence of 5 mM CaCl2 at a pH of 5.0 and 4.0, respectively. These properties indicate that the novel enzyme has a theoretically high survival rate and excellent starch catalytic efficiency in the typical chicken gastrointestinal-tract environment (pH 3.5-7.0, 40°C). In addition, the enzyme remained at 78.4 ± 3.6% of its activity after a 5-min incubation at 80°C, which demonstrates that the enzyme could maintain a high survival rate in the pelleting process of feed production. The characteristics just described make this enzyme a good candidate for use as a chicken feed enzyme.

A Novel Raw Starch Hydrolyzing Thermostable α-Amylase Produced by Newly Isolated Bacillus mojavensis SO-10: Purification, Characterization and Usage in Starch Industries

“…Lin et al 33 suggested that enzyme was activated with Ca 2+ , while it was inhibited in the presence of Hg 2+ . The effect of various metal ions on purified α-amylase activity was reported by Mamo and Gessesse 34 , Aguloglu Fincan and Enez 20 , and Liu et al 35 . Our results are very close to their studies.…”

Computational Insights into the Selecting Mechanism of α-Amylase Immobilized on Cellulose Nanocrystals: Unveiling the Potential of α-Amylases Immobilized for Efficient Poultry Feed Hydrolysis

“…One of the most widely used feed additives in the poultry industry is α-amylase, which can improve starch digestibility and poultry performance . It has been reported that acidic α-amylase from Bacillus subtilis is a good candidate for use as a chicken feed enzyme because of its high thermostability during the pelleting process of feed production . In another study, α-amylase from A.…”

“…63 It has been reported that acidic α-amylase from Bacillus subtilis is a good candidate for use as a chicken feed enzyme because of its high thermostability during the pelleting process of feed production. 64 In another study, α-amylase from A. oryzae was used as a supplement for ruminant diets to improve animal productivity by modifying starch. 65 Additionally, α-amylase was added in combination with other enzymes in the poultry diet to increase the digestibility of feed.…”