Identification and Analysis of Differential miRNAs in PK-15 Cells after Foot-and-Mouth Disease Virus Infection

Zhang, Ke-Shan; Liu, Yongjie; Kong, Hanjin; Cheng, Wei-Wei; Shang, Youjun; Tian, Hong; Zheng, Haixue; Guo, Jianpeng; Liu, Xian-Tao

doi:10.1371/journal.pone.0090865

Cited by 7 publications

(4 citation statements)

References 51 publications

(56 reference statements)

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“…In viral disease research, miRNA profiling has become a topic of considerable interest as researchers search for cellular targets that can be exploited in order to combat infection. In the case of FMDV, the sole miRNA profiling study to date was an investigation of the differential expression of miRNAs in a porcine based cell line in response to infection with an isolate of FMDV serotype Asia1 [49]. The widespread utility of these findings is somewhat limited given that cell culture is not necessarily reflective of the molecular pathogenesis of the virus in an in vivo setting.…”

Section: Discussionmentioning

confidence: 99%

“…The previously reported miRNA profile of a porcine cell line infected with FMDV serotype Asia1 was obtained using high throughput sequencing [49], which represents the first time such a differential profiling experiment was performed for FMDV. The present study represents the first miRNA profiling experiment, albeit a limited small scale proof-of-concept study, using serum collected from uninfected and infected cattle.…”

Section: Discussionmentioning

confidence: 99%

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Proof-of-concept study: profile of circulating microRNAs in Bovine serum harvested during acute and persistent FMDV infection

Stenfeldt

Arzt

Smoliga

et al. 2017

Virol J

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BackgroundChanges in the levels of circulating microRNAs (miRNAs) in the serum of humans and animals have been detected as a result of infection with a variety of viruses. However, to date, such a miRNA profiling study has not been conducted for foot-and-mouth disease virus (FMDV) infection.MethodsThe relative abundance of 169 miRNAs was measured in bovine serum collected at three different phases of FMDV infection in a proof-of-concept study using miRNA PCR array plates.ResultsAlterations in specific miRNA levels were detected in serum during acute, persistent, and convalescent phases of FMDV infection. Subclinical FMDV persistence produced a circulating miRNA profile distinct from cattle that had cleared infection. bta-miR-17-5p was highest expressed during acute infection, whereas bta-miR-31 was the highest during FMDV persistence. Interestingly, miR-1281was significantly down-regulated during both acute and persistent infection. Cattle that cleared infection resembled the baseline profile, adding support to applying serum miRNA profiling for identification of sub-clinically infected FMDV carriers. Significantly regulated miRNAs during acute or persistent infection were associated with cellular proliferation, apoptosis, modulation of the immune response, and lipid metabolism.ConclusionsThese findings suggest a role for non-coding regulatory RNAs in FMDV infection of cattle. Future studies will delineate the individual contributions of the reported miRNAs to FMDV replication, determine if this miRNA signature is applicable across all FMDV serotypes, and may facilitate development of novel diagnostic applications.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-017-0743-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proof-of-concept study: profile of circulating microRNAs in Bovine serum harvested during acute and persistent FMDV infection

Stenfeldt

Arzt

Smoliga

et al. 2017

Virol J

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“…These studies emphasize that miR-29b can modulate the pathogenesis of bovine viral diarrhea virus using different mechanisms. Along the same lines, PK-15 cells were used to screen for differentially expressed miRNAs during foot-and-mouth disease virus (FMDV) infection ( 166 ). The analysis revealed that 172 known miRNAs and 72 putative miRNAs were differentially expressed, the majority of which were downregulated.…”

Section: Micrornas In the Host Response To Viral Infections Of Veterimentioning

confidence: 99%

MicroRNAs in the Host Response to Viral Infections of Veterinary Importance

2016

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The discovery of small regulatory non-coding RNAs has been an exciting advance in the field of genomics. MicroRNAs (miRNAs) are endogenous RNA molecules, approximately 22 nucleotides in length, that regulate gene expression, mostly at the posttranscriptional level. MiRNA profiling technologies have made it possible to identify and quantify novel miRNAs and to study their regulation and potential roles in disease pathogenesis. Although miRNAs have been extensively investigated in viral infections of humans, their implications in viral diseases affecting animals of veterinary importance are much less understood. The number of annotated miRNAs in different animal species is growing continuously, and novel roles in regulating host–pathogen interactions are being discovered, for instance, miRNA-mediated augmentation of viral transcription and replication. In this review, we present an overview of synthesis and function of miRNAs and an update on the current state of research on host-encoded miRNAs in the genesis of viral infectious diseases in their natural animal host as well as in selected in vivo and in vitro laboratory models.

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“…In the PK‐15 porcine kidney cell line, FMDV Asia 1 induced differential expression of 172 known, and 72 novel, miRNAs after 6 h, compared to non‐infected cells. Pathway annotation revealed that the miRNAs were abundant in cell death and immune response pathways (Zhang et al., ). In addition, another group working in China employed high‐throughput quantitative proteomics to analyse the global changes in protein expression in FMDV‐infected IBRS‐2 cells.…”

Section: Molecular Biologymentioning

confidence: 99%

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 7 - Pathogenesis and Molecular Biology

Robinson

Knight-Jones

Charleston

et al. 2016

Transbound Emerg Dis

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SummaryWe assessed research knowledge gaps in the fields of FMDV (foot-and-mouth disease virus) pathogenesis and molecular biology by performing a literature review and collecting research updates (2014) from 33 institutes from across the world. Findings were used to identify priority areas for future research. There have been important advances in FMDV pathogenesis; FMDV remains in lymph nodes of many recovered animals that otherwise do not appear persistently infected, even in species previously not associated with the carrier state. Whether virus retention helps maintain host immunity and/or virus survival is not known. Studies of FMDV pathogenesis in wildlife have provided insights into disease epidemiology, in endemic and epidemic settings. Many aspects of FMDV infection and virus entry remain unknown; however, at the cellular level, we know that expression level and availability of integrins (that permit viral entry), rate of clearance of infected cells and strength of anti-viral type I IFN (interferon) response are key determinants of tissue tropism. Extending findings to improved understanding of transmission requires a standardized approach and adoption of natural routes of infection during experimental study. There has been recognition of the importance of autophagosomes for FMDV entry into the cytoplasm following cell surface receptor binding, and that distinct internal cellular membranes are exploited for viral replication and immune evasion. New roles for viral proteins in blocking type I IFN production and downstream signalling have been identified facilitating research in anti-viral therapeutics. We know more about how infection affects cell protein expression, and research into molecular determinants of capsid stability has aided the development of stable vaccines. We have an expanding knowledge of viral and host molecular determinates of virulence and infectiousness, and of how phylogenetics may be used to estimate vaccine match and strain distribution. With ongoing advances, these areas could translate into significantly improved disease control.

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Identification and Analysis of Differential miRNAs in PK-15 Cells after Foot-and-Mouth Disease Virus Infection

Cited by 7 publications

References 51 publications

Proof-of-concept study: profile of circulating microRNAs in Bovine serum harvested during acute and persistent FMDV infection

Proof-of-concept study: profile of circulating microRNAs in Bovine serum harvested during acute and persistent FMDV infection

MicroRNAs in the Host Response to Viral Infections of Veterinary Importance

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 7 - Pathogenesis and Molecular Biology

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