The dependence of signal-to-noise ratio on number of scans in covariance spectroscopy

Qian, Yi; Shen, Ming; Amoureux, Jean‐Paul; Noda, Isao; Hu, Bingwen

doi:10.1016/j.ssnmr.2014.02.002

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…It cannot be applied to proteins, contrary to the covariance method, which is another way to decrease the acquisition time of HOMCOR spectra in liquid [ 29–33 ] or solid samples. [ 34–37 ]…”

Section: Discussionmentioning

confidence: 99%

“…It cannot be applied to proteins, contrary to the covariance method, which is another way to decrease the acquisition time of HOMCOR spectra in liquid [29][30][31][32][33] or solid samples. [34][35][36][37] Thirdly, this method better applies to the case of narrow resonances, which are fully resolved. It should not be used with broad resonances resulting from distribution of surroundings or quadrupolar nuclei.…”

Section: Discussionmentioning

confidence: 99%

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Hadamard acquisition of ¹³C–¹³C 2‐D correlation NMR spectra

Paluch

Kupče

Trébosc

et al. 2019

Magnetic Reson in Chemistry

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We show that a multiselective excitation with Hadamard encoding is a powerful tool for 2-D acquisition of 13 C─ 13 C homonuclear correlations. This method is not designed to improve the sensitivity, but rather to reduce the experiment time, provided there is sufficient sensitivity. Therefore, it allows fast acquisition of such 2-D spectra in labeled molecules. The technique has been demonstrated using a U─ 13 C─ 15 N histidine hydrochloride monohydrate sample allowing each point of the build-up curves of the 13 C─ 13 C cross-peaks to be recorded within 4 min 35 s, which is very difficult with conventional methods. Using the U─ 13 C─ 15 N f-MLF sample, we have demonstrated that the method can be applied to molecules with 14 13 C resonances with a minimum frequency separation of 240 Hz.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hadamard acquisition of ¹³C–¹³C 2‐D correlation NMR spectra

Paluch

Kupče

Trébosc

et al. 2019

Magnetic Reson in Chemistry

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“…To plot the 2D [ 1 H- 15 N] HSQC ratios of PYK2 KFL 728–839 peak intensities at 10 µM divided by those at 100 µM, the intensity values were retrieved by exporting data height with signal-to-noise values using NMRFAM-SPARKY 74 . The intensity and signal-to-noise values for both concentrations of PYK2 KFL 728–839 were normalised according to the number of scans 75 , 76 .…”

Section: Methodsmentioning

confidence: 99%

PYK2 senses calcium through a disordered dimerization and calmodulin-binding element

et al. 2022

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Multidomain kinases use many ways to integrate and process diverse stimuli. Here, we investigated the mechanism by which the protein tyrosine kinase 2-beta (PYK2) functions as a sensor and effector of cellular calcium influx. We show that the linker between the PYK2 kinase and FAT domains (KFL) encompasses an unusual calmodulin (CaM) binding element. PYK2 KFL is disordered and engages CaM through an ensemble of transient binding events. Calcium increases the association by promoting structural changes in CaM that expose auxiliary interaction opportunities. KFL also forms fuzzy dimers, and dimerization is enhanced by CaM binding. As a monomer, however, KFL associates with the PYK2 FERM-kinase fragment. Thus, we identify a mechanism whereby calcium influx can promote PYK2 self-association, and hence kinase-activating trans-autophosphorylation. Collectively, our findings describe a flexible protein module that expands the paradigms for CaM binding and self-association, and their use for controlling kinase activity.

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“…For the characterization of solids, covariance processing has been used for the reconstruction of 13 C homonuclear 1Q–1Q correlation 2D spectra of crystalline aminoacids or peptides, microcrystalline proteins and spider threads . We have also shown that covariance allows processing 13 C homonuclear 2Q–1Q correlation 2D spectrum of microcrystalline protein (see Fig.…”

Section: Sparse Samplingmentioning

confidence: 99%

Fast acquisition of multidimensional NMR spectra of solids and mesophases using alternative sampling methods

Lesot

Kazimierczuk

Trébosc

et al. 2015

Magnetic Reson in Chemistry

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Unique information about the atom-level structure and dynamics of solids and mesophases can be obtained by the use of multidimensional nuclear magnetic resonance (NMR) experiments. Nevertheless, the acquisition of these experiments often requires long acquisition times. We review here alternative sampling methods, which have been proposed to circumvent this issue in the case of solids and mesophases. Compared to the spectra of solutions, those of solids and mesophases present some specificities because they usually display lower signal-to-noise ratios, non-Lorentzian line shapes, lower spectral resolutions and wider spectral widths. We highlight herein the advantages and limitations of these alternative sampling methods. A first route to accelerate the acquisition time of multidimensional NMR spectra consists in the use of sparse sampling schemes, such as truncated, radial or random sampling ones. These sparsely sampled datasets are generally processed by reconstruction methods differing from the Discrete Fourier Transform (DFT). A host of non-DFT methods have been applied for solids and mesophases, including the G-matrix Fourier transform, the linear least-square procedures, the covariance transform, the maximum entropy and the compressed sensing. A second class of alternative sampling consists in departing from the Jeener paradigm for multidimensional NMR experiments. These non-Jeener methods include Hadamard spectroscopy as well as spatial or orientational encoding of the evolution frequencies. The increasing number of high field NMR magnets and the development of techniques to enhance NMR sensitivity will contribute to widen the use of these alternative sampling methods for the study of solids and mesophases in the coming years.

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The dependence of signal-to-noise ratio on number of scans in covariance spectroscopy

Cited by 7 publications

References 21 publications

Hadamard acquisition of ¹³C–¹³C 2‐D correlation NMR spectra

Hadamard acquisition of ¹³C–¹³C 2‐D correlation NMR spectra

PYK2 senses calcium through a disordered dimerization and calmodulin-binding element

Fast acquisition of multidimensional NMR spectra of solids and mesophases using alternative sampling methods

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The dependence of signal-to-noise ratio on number of scans in covariance spectroscopy

Cited by 7 publications

References 21 publications

Hadamard acquisition of 13C–13C 2‐D correlation NMR spectra

Hadamard acquisition of 13C–13C 2‐D correlation NMR spectra

PYK2 senses calcium through a disordered dimerization and calmodulin-binding element

Fast acquisition of multidimensional NMR spectra of solids and mesophases using alternative sampling methods

Contact Info

Product

Resources

About

Hadamard acquisition of ¹³C–¹³C 2‐D correlation NMR spectra

Hadamard acquisition of ¹³C–¹³C 2‐D correlation NMR spectra