Disk-Shaped Amperometric Enzymatic Biosensor for in Vivo Detection of <scp>d</scp>-serine

Polcari, David; Kwan, Annie; Horn, Marion R. Van; Danis, Laurence; Pollegioni, Loredano; Ruthazer, Edward S.; Mauzeroll, Janine

doi:10.1021/ac404111u

Cited by 32 publications

(40 citation statements)

References 44 publications

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“…However, the results obtained with biosensors should be interpreted with caution. D-Serine biosensors also detect most neutral D-amino acids, including D-alanine, and might non-specifically oxidize other biological compounds [48]. Although D-alanine levels in the hippocampus are much lower than D-serine [49], the catalytic efficiency of DAO enzymes for D-alanine is about 40 times higher than of D-serine [50], so it is conceivable that DAO-based biosensors can detect trace amounts of D-alanine.…”

Section: D-serine Is Not a Gliotransmittermentioning

confidence: 99%

The Rise and Fall of the d -Serine-Mediated Gliotransmission Hypothesis

Wolosker

Balu

Coyle

2016

Trends in Neurosciences

159

152

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D-Serine modulates NMDA receptors and regulates synaptic plasticity, neurodevelopment, and learning and memory. However, the primary site of D-serine synthesis and release remains controversial, with some arguing that it is a “gliotransmitter” and others defining it as a “neuronal co-transmitter”. Results from several laboratories using different strategies now show that the D-serine’s biosynthetic enzyme, serine racemase (SR), is expressed almost entirely by neurons, with few astrocytes appearing to contain D-serine. Cell-selective suppression of serine racemase expression demonstrates that neuronal, rather than astrocytic D-serine, modulates synaptic plasticity. Here we propose an alternative conceptualization whereby astrocytes affect D-serine levels by synthesizing L-serine that shuttles to neurons to fuel the neuronal synthesis of D-serine.

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Section: D-serine Is Not a Gliotransmittermentioning

confidence: 99%

The Rise and Fall of the d -Serine-Mediated Gliotransmission Hypothesis

Wolosker

Balu

Coyle

2016

Trends in Neurosciences

159

152

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“…Pt disk microelectrodes, used as backbones for the biosensors, were fabricated using a previously reported procedure . Briefly, a soda‐lime glass capillary was pulled and a Pt wire (25 μm diameter) was inserted into the capillary.…”

Section: Methodsmentioning

confidence: 99%

“…We recently reported the use of an enzymatic amperometric biosensor to measure the evoked release of endogenous d ‐serine in the brain of stage 48 albino Xenopus laevis tadpoles . The biosensor architecture included a 25 μm diameter platinum disk microelectrode backbone, a permselective poly( meta ‐phenylenediamine) (PPD) polymer layer, and the highly active enzyme Rhodotorula gracilis d ‐amino acid oxidase (DAAO, EC 1.4.3.3).…”

Section: Introductionmentioning

confidence: 99%

Localized Detection of d‐Serine by using an Enzymatic Amperometric Biosensor and Scanning Electrochemical Microscopy

et al. 2017

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d‐Serine acts as an endogenous co‐agonist for N‐methyl‐d‐aspartate receptors at synapses, making it essential for proper brain development and function. This amino acid has also been linked to several neurodegenerative diseases such as Alzheimer's disease and dementia. Nevertheless, the primary site and mechanism of d‐serine release remains unclear. We recently demonstrated the use of an enzymatic amperometric biosensor for the in vivo quantification of endogenous d‐serine release in Xenopus laevis tadpoles. Herein, we investigate the effect of the permselective poly(meta‐phenylenediamine) electropolymerization conditions on the biosensor's response time and selectivity. Scanning electrochemical microscopy (SECM) is then used with the optimized biosensor to measure localized release of d‐serine from a model system. This SECM methodology, which provides high spatial and temporal resolution, could be useful to investigate the primary site and mechanism of d‐serine release in other biological samples.

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“…Enzymes can be attached to the electrode surface directly, by covalent bonding, or by entrapment of the enzyme in a polymer film over the electrode surface. Immobilized enzyme sensors for species-selective have been implemented in the SECM [82], for example an enzymatic amperometric biosensor was used to measure the release of endogenous D-serine in the brain of stage 48 albino Xenopus laevis tadpoles [83]. This electrode had an appropriate dimension (25 µm diameter), and gave good temporal resolution to be used as a probe in SECM [84].…”

Section: Enzyme Modified Probes For Chemical Imaging In Scanning Elecmentioning

confidence: 99%

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Lazenby

White

2018

Chemosensors

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This review discusses a broad range of recent advances (2013)(2014)(2015)(2016)(2017) in chemical imaging using electrochemical methods, with a particular focus on techniques that have been applied to study cellular processes, or techniques that show promise for use in this field in the future. Non-scanning techniques such as microelectrode arrays (MEAs) offer high time-resolution (<10 ms) imaging; however, at reduced spatial resolution. In contrast, scanning electrochemical probe microscopies (SEPMs) offer higher spatial resolution (as low as a few nm per pixel) imaging, with images collected typically over many minutes. Recent significant research efforts to improve the spatial resolution of SEPMs using nanoscale probes and to improve the temporal resolution using fast scanning have resulted in movie (multiple frame) imaging with frame rates as low as a few seconds per image. Many SEPM techniques lack chemical specificity or have poor selectivity (defined by the choice of applied potential for redox-active species). This can be improved using multifunctional probes, ion-selective electrodes and tip-integrated biosensors, although additional effort may be required to preserve sensor performance after miniaturization of these probes. We discuss advances to the field of electrochemical imaging, and technological developments which are anticipated to extend the range of processes that can be studied. This includes imaging cellular processes with increased sensor selectivity and at much improved spatiotemporal resolution than has been previously customary.

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Disk-Shaped Amperometric Enzymatic Biosensor for in Vivo Detection of d-serine

Cited by 32 publications

References 44 publications

The Rise and Fall of the d -Serine-Mediated Gliotransmission Hypothesis

The Rise and Fall of the d -Serine-Mediated Gliotransmission Hypothesis

Localized Detection of d‐Serine by using an Enzymatic Amperometric Biosensor and Scanning Electrochemical Microscopy

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

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