Remote‐controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment

Nejdl, Lukáš; Kudr, Jiří; Číhalová, Kristýna; Chudobová, Dagmar; Žůrek, Michal; Žalud, Luděk; Kopecny, L.; Burian, Frantisek; Ruttkay-Nedecký, Branislav; Křížková, Soňa; Konečná, Marie; Hynek, David; Kopel, Pavel; Prášek, Jan; Adam, Vojtěch; Kizek, René

doi:10.1002/elps.201300576

Cited by 26 publications

(18 citation statements)

References 64 publications

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“…Nevertheless, they require expensive instrumentation, experienced operators, and the analyses are time-consuming. On the contrary, electrochemistry offers superior features like portability, easy use, low price, miniaturization, and high sensitivity [19,20,21,22,23]. The great advantage of electroanalysis is also the possibility of electrode surface modification [24].…”

Section: Introductionmentioning

confidence: 99%

Improved Electrochemical Detection of Zinc Ions Using Electrode Modified with Electrochemically Reduced Graphene Oxide

et al. 2016

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Increasing urbanization and industrialization lead to the release of metals into the biosphere, which has become a serious issue for public health. In this paper, the direct electrochemical reduction of zinc ions is studied using electrochemically reduced graphene oxide (ERGO) modified glassy carbon electrode (GCE). The graphene oxide (GO) was fabricated using modified Hummers method and was electrochemically reduced on the surface of GCE by performing cyclic voltammograms from 0 to −1.5 V. The modification was optimized and properties of electrodes were determined using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The determination of Zn(II) was performed using differential pulse voltammetry technique, platinum wire as a counter electrode, and Ag/AgCl/3 M KCl reference electrode. Compared to the bare GCE the modified GCE/ERGO shows three times better electrocatalytic activity towards zinc ions, with an increase of reduction current along with a negative shift of reduction potential. Using GCE/ERGO detection limit 5 ng·mL−1 was obtained.

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Section: Introductionmentioning

confidence: 99%

Improved Electrochemical Detection of Zinc Ions Using Electrode Modified with Electrochemically Reduced Graphene Oxide

et al. 2016

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“…It can be used in rescue robotics [68] or experimental biology [69]. In future work, it could be developed with more sensors with data fusion [70], what would allow not only monitoring the oedema, but also find the centre of inflammation due to augmented reality [71].…”

Section: Discussionmentioning

confidence: 99%

Application of High-Resolution 3D Scanning in Medical Volumetry

Chromý¹

2016

International Journal of Electronics and Telecommunications

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Abstract-This paper deals with application of 3D scanning technology in medicine. Important properties of 3D scanners are discussed with emphasize on medical applications. Construction of medical 3D scanner according to these specifications is described and practical application of its use in medical volumetry is presented. Besides volumetry, such 3D scanner is usable for many other purposes, like monitoring of recovery process, ergonomic splint manufacturing or inflammation detection.3D scanning introduces novel volumetric method, which is compared with standard methods. The new method is more accurate compared to present ones. Principles of this method are discussed in paper and its accuracy is evaluated and experimentally verified.

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“…Sodium borohydride (1 g) dissolved in 3.5% ammonia (50 mL) was poured to stirred solution of iron(III) nitrate nonahydrate (7.48 g) dissolved in water (400 mL) [29]. The mixture was then heated at 100 °C for 2 h. The dark product was separated by magnet and washed several times with water.…”

Section: Methodsmentioning

confidence: 99%

Construction of an Acetylcholinesterase Sensor Based on Synthesized Paramagnetic Nanoparticles, a Simple Tool for Neurotoxic Compounds Assay

Kostelník

Kopel

Cegan

et al. 2017

Sensors

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Magnetic particles (MPs) have been widely used in biological applications in recent years as a carrier for various molecules. Their big advantage is in repeated use of immobilized molecules including enzymes. Acetylcholinesterase (AChE) is an enzyme playing crucial role in neurotransmission and the enzyme is targeted by various molecules like Alzheimer’s drugs, pesticides and warfare agents. In this work, an electrochemical biosensor having AChE immobilized onto MPs and stabilized through glutaraldehyde (GA) molecule was proposed for assay of the neurotoxic compounds. The prepared nanoparticles were modified by pure AChE and they were used for the measurement anti-Alzheimer’s drug galantamine and carbamate pesticide carbofuran with limit of detection 1.5 µM and 20 nM, respectively. All measurements were carried out using screen-printed sensor with carbon working, silver reference, and carbon auxiliary electrode. Standard Ellman’s assay was used for validation measurement of both inhibitors. Part of this work was the elimination of reversible inhibitors represented by galantamine from the active site of AChE. For this purpose, we used a lower pH to get the original activity of AChE after inhibition by galantamine. We also observed decarbamylation of the AChE-carbofuran adduct. Influence of organic solvents to AChE as well as repeatability of measurement with MPs with AChE was also established.

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Remote‐controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment

Cited by 26 publications

References 64 publications

Improved Electrochemical Detection of Zinc Ions Using Electrode Modified with Electrochemically Reduced Graphene Oxide

Improved Electrochemical Detection of Zinc Ions Using Electrode Modified with Electrochemically Reduced Graphene Oxide

Application of High-Resolution 3D Scanning in Medical Volumetry

Construction of an Acetylcholinesterase Sensor Based on Synthesized Paramagnetic Nanoparticles, a Simple Tool for Neurotoxic Compounds Assay

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