Mangiferin Has an Additive Effect on the Apoptotic Properties of Hesperidin in Cyclopia sp. Tea Extracts

Bartoszewski, Rafał; Hering, Anna; Marszałł, Michał Piotr; Hajduk, Justyna Stefanowicz; Bartoszewska, Sylwia; Kapoor, Niren; Kochan, Kinga; Ochocka, Renata

doi:10.1371/journal.pone.0092128

Cited by 51 publications

(42 citation statements)

References 47 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used TaqManOne-Step RT-PCR Master MixReagents (Applied Biosystems) as described previously (Bartoszewska et al, 2013; Bartoszewski et al, 2011; Bartoszewski et al, 2014) using the manufacturer’s protocol. The relative expressions were calculated using the comparative relative standard curve method (Larionov et al, 2005).…”

Section: Methodsmentioning

confidence: 99%

miR-200b downregulates Kruppel Like Factor 2 (KLF2) during acute hypoxia in human endothelial cells

Bartoszewski

Serocki

Janaszak-Jasiecka

et al. 2017

European Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

The role of microRNAs in controlling angiogenesis is recognized as a promising therapeutic target in both cancer and cardiovascular disorders. However, understanding a miRNA’s pleiotropic effects on angiogenesis is a limiting factor for these types of therapeutic approaches. Using genome-wide next-generation sequencing, we examined the role of an antiangiogenic miRNA, miR-200b, in primary human endothelial cells. The results indicate that miR-200b has complex effects on hypoxia-induced angiogenesis in human endothelia and importantly, that many of the reported miR-200b effects using miRNA overexpression may not be representative of the physiological role of this miRNA. We also identified the antiangiogenic KLF2 gene as a novel target of miR-200b. Our studies indicate that the physiological changes in miR-200b levels during acute hypoxia may actually have a proangiogenic effect through Klf2 downregulation and subsequent stabilization of HIF-1 signaling. Moreover, we provide a viable approach for differentiating direct from indirect miRNA effects in order to untangle the complexity of individual miRNA networks.

show abstract

Section: Methodsmentioning

confidence: 99%

miR-200b downregulates Kruppel Like Factor 2 (KLF2) during acute hypoxia in human endothelial cells

Bartoszewski

Serocki

Janaszak-Jasiecka

et al. 2017

European Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…RNA samples were stored at − 70 °C until use. For the measurement of CD39, CD73, ADA, ADORA1, ADORA2a, ADORA2b and ADORA3 mRNA expression, TaqManOne-Step RT-PCR Master MixReagents (Applied Biosystems, USA) were used as described previously [37,38] according to the manufacturer's protocol. The relative expressions were calculated using the comparative relative standard curve method [39].…”

Section: Gene Expressionmentioning

confidence: 99%

Nucleotide ecto-enzyme metabolic pattern and spatial distribution in calcific aortic valve disease; its relation to pathological changes and clinical presentation

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background Extracellular nucleotide metabolism contributes to chronic inflammation, cell differentiation, and tissue mineralization by controlling nucleotide and adenosine concentrations and hence its purinergic effects. This study investigated location-specific changes of extracellular nucleotide metabolism in aortic valves of patients with calcific aortic valve disease (CAVD). Individual ecto-enzymes and adenosine receptors involved were analyzed together with correlation with CAVD severity and risk factors. Results Nucleotide and adenosine degradation rates were adversely modified on the aortic surface of stenotic valve as compared to ventricular side, including decreased ATP removal (1.25 ± 0.35 vs. 2.24 ± 0.61 nmol/min/cm 2 ) and adenosine production (1.32 ± 0.12 vs. 2.49 ± 0.28 nmol/min/cm 2 ) as well as increased adenosine deamination (1.28 ± 0.31 vs. 0.67 ± 0.11 nmol/min/cm 2 ). The rates of nucleotide to adenosine conversions were lower, while adenosine deamination was higher on the aortic sides of stenotic vs. non-stenotic valve. There were no differences in extracellular nucleotide metabolism between aortic and ventricular sides of non-stenotic valves. Furthermore, nucleotide degradation rates, measured on aortic side in CAVD (n = 62), negatively correlated with echocardiographic and biochemical parameters of disease severity (aortic jet velocity vs. ATP hydrolysis: r = − 0.30, p < 0.05; vs. AMP hydrolysis: r = − 0.44, p < 0.001; valvular phosphate concentration vs. ATP hydrolysis: r = − 0.26, p < 0.05; vs. AMP hydrolysis: r = − 0.25, p = 0.05) while adenosine deamination showed positive correlation trend with valvular phosphate deposits (r = 0.23, p = 0.07). Nucleotide and adenosine conversion rates also correlated with CAVD risk factors, including hyperlipidemia (AMP hydrolysis vs. serum LDL cholesterol: r = − 0.28, p = 0.05; adenosine deamination vs. total cholesterol: r = 0.25, p = 0.05; LDL cholesterol: r = 0.28, p < 0.05; triglycerides: r = 0.32, p < 0.05), hypertension (adenosine deamination vs. systolic blood pressure: r = 0.28, p < 0.05) and thrombosis (ATP

show abstract

“…We used TaqMan One-Step RT-PCR Master Mix Reagents (Applied Biosystems, Foster City, CA, USA) as described previously (18)(19)(20) using the manufacturer's protocol (relative quantification; Applied Biosystems StepOnePlus Real-Time PCR System). The relative expressions were calculated using the comparative relative standard curve method (21).…”

Section: Isolation Of Rna and Mirnamentioning

confidence: 99%

The hypoxia‐inducible miR‐429 regulates hypoxia‐inducible factor‐1α expression in human endothelial cells through a negative feedback loop

et al. 2014

Self Cite

View full text Add to dashboard Cite

Hypoxia-inducible factors (HIFs) 1 and 2 are dimeric α/β transcription factors that regulate cellular responses to low oxygen. HIF-1 is induced first, whereas HIF-2 is associated with chronic hypoxia. To determine how HIF1A mRNA, the inducible subunit of HIF-1, is regulated during hypoxia, we followed HIF1A mRNA levels in primary HUVECs over 24 hours using quantitative PCR. HIF1A and VEGF A (VEGFA) mRNA, a transcriptional target of HIF-1, increased ∼ 2.5- and 8-fold at 2-4 hours, respectively. To determine how the mRNAs were regulated, we identified a microRNA (miRNA), miR-429, that destabilized HIF1A message and decreased VEGFA mRNA by inhibiting HIF1A. Target protector analysis, which interferes with miRNA-mRNA complex formation, confirmed that miR-429 targeted HIF1A message. Desferoxamine treatment, which inhibits the hydroxylases that promote HIF-1α protein degradation, stabilized HIF-1 activity during normoxic conditions and elevated miR-429 levels, demonstrating that HIF-1 promotes miR-429 expression. RNA-sequencing-based transcriptome analysis indicated that inhibition of miRNA-429 in HUVECs up-regulated 209 mRNAs, a number of which regulate angiogenesis. The results demonstrate that HIF-1 is in a negative regulatory loop with miR-429, that miR-429 attenuates HIF-1 activity by decreasing HIF1A message during the early stages of hypoxia before HIF-2 is activated, and this regulatory network helps explain the HIF-1 transition to HIF-2 during chronic hypoxia in endothelial cells.

show abstract

Mangiferin Has an Additive Effect on the Apoptotic Properties of Hesperidin in Cyclopia sp. Tea Extracts

Cited by 51 publications

References 47 publications

miR-200b downregulates Kruppel Like Factor 2 (KLF2) during acute hypoxia in human endothelial cells

miR-200b downregulates Kruppel Like Factor 2 (KLF2) during acute hypoxia in human endothelial cells

Nucleotide ecto-enzyme metabolic pattern and spatial distribution in calcific aortic valve disease; its relation to pathological changes and clinical presentation

The hypoxia‐inducible miR‐429 regulates hypoxia‐inducible factor‐1α expression in human endothelial cells through a negative feedback loop

Contact Info

Product

Resources

About