Dual track time‐of‐flight mass spectrometry for peptide quantification with matrix‐assisted laser desorption/ionization

Bae, Yong Jin; Moon, Jeong Hee; Kim, Myoung Soo

doi:10.1002/rcm.6845

Cited by 4 publications

(4 citation statements)

References 29 publications

(55 reference statements)

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“…A homebuilt MALDI-TOF instrument [4] was used in this work. It consists of an ion source with delayed extraction, a linear TOF analyzer, and a deflector.…”

Section: Methodsmentioning

confidence: 99%

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Gain Switching for a Detection System to Accommodate a Newly Developed MALDI-Based Quantification Method

Ahn

Hyeon

Kim

et al. 2017

J. Am. Soc. Mass Spectrom.

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In matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF), matrix-derived ions are routinely deflected away to avoid problems with ion detection. This, however, limits the use of a quantification method that utilizes the analyte-to-matrix ion abundance ratio. In this work, we will show that it is possible to measure this ratio by a minor instrumental modification of a simple form of MALDI-TOF. This involves detector gain switching. Graphical Abstract ᅟ.

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“…A homebuilt MALDI-TOF instrument [4] was used in this work. It consists of an ion source with delayed extraction, a linear TOF analyzer, and a deflector.…”

Section: Methodsmentioning

confidence: 99%

“…This is costly. An alternative is to provide two different tracks and detection systems, one for the matrixderived ions and the other for the analyte-derived ions [4]. This dual-track instrument is even more costly.…”

Section: Construction Of the Calibration Curve By Plotting I([a + H] + )/ I([m + H] + ) Versus C([a]mentioning

confidence: 99%

Gain Switching for a Detection System to Accommodate a Newly Developed MALDI-Based Quantification Method

Ahn

Hyeon

Kim

et al. 2017

J. Am. Soc. Mass Spectrom.

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show abstract

“…In our homebuilt MALDI-TOF, we avoid this problem either by using a two-channel analog-to-digital converter or by using detectors at two different locations. 12 Such instruments are not commercially available. In this work, we present modifications of our original method which do not require matrix ion signals and hence can be used in unmodified commercial instruments and evaluate their performance.…”

Section: Ahmentioning

confidence: 99%

“…This results in a loss of information on the matrix ion abundances. In our homebuilt MALDI‐TOF, we avoid this problem either by using a two‐channel analog‐to‐digital converter or by using detectors at two different locations . Such instruments are not commercially available.…”

Section: Introductionmentioning

confidence: 99%

Use of Commercial MALDI‐TOFs for Analyte Quantification by a Newly Invented Method

Park

Yoon

Moon

et al. 2016

Bulletin Korean Chem Soc

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As part of the recently developed quantification method using matrix-assisted laser desorption ionization, matrix ion abundances are used to control the effective temperature, to construct a calibration curve, and to check the reliability of the quantification results. This method has not been used with commercial instruments owing to the difficulties in measuring the matrix ion abundances. In this work, we present a modification of each of the above guidelines to allow for quantification with commercial instruments. The modified method works with commercial instruments, which is partial proof for the validity of the method.

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Tracing the Origin of the Anomalous Matrix Suppression in Matrix‐assisted Laser Desorption Ionization and Extending the Linear Dynamic Range During Analyte Quantification

Song

Hyeon

Kim

et al. 2018

Bulletin Korean Chem Soc

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In the matrix‐assisted laser desorption ionization‐based quantification method developed in this laboratory, a linear calibration curve is constructed by plotting the analyte‐to‐matrix ion abundance ratio as a function of the analyte concentration. As the analyte concentration increases, however, the curve deviates from linearity. Previously, we observed that the occurrence of this deviation correlated with the decrease in the matrix ion signal, or matrix suppression. In this work, we found that saturation of the detector was one of the factors responsible for the above phenomena. As we decreased the detector gain, the upper limit of the linear dynamic range increased by as much as one order of magnitude. Even after a substantial decrease in the detector gain, however, the nonlinearity remained at a high analyte concentration, which we cannot account for at present. The method developed in this work can be useful for the quantitative imaging of analytes in heavily contaminated samples.

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Dual track time‐of‐flight mass spectrometry for peptide quantification with matrix‐assisted laser desorption/ionization

Abstract: We devised the dual track MALDI-TOF MS method to avoid detector saturation and demonstrated that the quantification and identification of peptides can be performed simultaneously.

Cited by 4 publications

References 29 publications

Gain Switching for a Detection System to Accommodate a Newly Developed MALDI-Based Quantification Method

Gain Switching for a Detection System to Accommodate a Newly Developed MALDI-Based Quantification Method

Use of Commercial MALDI‐TOFs for Analyte Quantification by a Newly Invented Method

Tracing the Origin of the Anomalous Matrix Suppression in Matrix‐assisted Laser Desorption Ionization and Extending the Linear Dynamic Range During Analyte Quantification

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