Optimization and evaluation of a bottom substrate denitrification tank for nitrate removal from a recirculating aquaculture system

Pungrasmi, Wiboonluk; Playchoom, Chonthicha; Powtongsook, Sorawit

doi:10.1016/s1001-0742(12)60248-4

Cited by 24 publications

(8 citation statements)

References 21 publications

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“…Aquaculture tanks were covered by a transparent plastic sheet to minimize oxygen diffusion from the atmosphere. Methanol was supplied as an external organic carbon source for denitrifiers at a chemical oxygen demand (COD): nitrate-N ratio of 5:1 [15] on day 61 of the experiment. More methanol was added if needed when residual nitrate was found.…”

Section: Biofilter Acclimation and Ras Operationmentioning

confidence: 99%

Effects of Salinity and Immobilization Period on the Nitrification and Denitrification Co-processes during Biofilter Acclimation in a Marine Recirculating Aquaculture System

Satanwat

Pungrasmi

Powtongsook

2019

J. of Wat. & Envir. Tech.

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Biological filtration plays an important role in a sustainable recirculating aquaculture system. To provide the appropriate conditions for biofilter acclimation, the effects of the salinity and acclimation period on nitrogen removal and microbial diversity were evaluated. Nitrification rate (NR) of the shrimp tank biofilter increased during the first 4 weeks and decreased thereafter. The highest NR was found in the 5 PSU salinity tank at week 2, whereas in the other salinities (15 and 25 PSU) the highest NR was observed at week 4. Meanwhile, the denitrification rate (DNR) increased significantly on week 8 and the highest DNRs were obtained after switching from an aerobic to an anoxic condition with methanol supplement. Under this anoxic condition the biofilter acclimated in seawater had the highest DNR. Changes in the microbial community during immobilization were monitored using Illumina MiSeq DNA sequencing. Proteobacteria and Bacteriodetes were the most abundant phyla found in this study. For nitrifiers, Nitrosomonadaceae were found from the first week of acclimation, while only one operational taxonomic unit (OTU) of Nitrospiraceae was observed. Under the anoxic condition at the end of the shrimp culture, two OTUs of Nitrospiraceae were found in both the 15 and 25 PSU tanks.

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Section: Biofilter Acclimation and Ras Operationmentioning

confidence: 99%

Effects of Salinity and Immobilization Period on the Nitrification and Denitrification Co-processes during Biofilter Acclimation in a Marine Recirculating Aquaculture System

Satanwat

Pungrasmi

Powtongsook

2019

J. of Wat. & Envir. Tech.

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“…Biological nitrogen removal technology is an efficient process for nitrogen removal from aquatic water in which probiotic bacteria convert nitrite-N into nitrate nitrogen (nitrate-N) or nitrogen gas. Moreover, due to lower energy consumption and operation cost, the technology is commonly used in aquaculture (Van Rijn, Tal & Schreier 2006;Pungrasmi, Playchoom & Powtongsook 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification and real-time qPCR quantification of a nitrite-N degrading bacterial strain in aquatic water

Pan

et al. 2016

Aquac Res

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Biological nitrogen removal technology using microbe is an efficient process for nitrogen removal from aquatic water. To determine the key of efficient application on how the effect of probiotics lasting, a nitrite nitrogen (nitrite‐N) degrading 8DO17 strain Pseudomonas was screened and a method for quantification was explored. Real‐time qPCR assays based on the 16S rRNA genes (16S rDNA) were used to quantify the probiotics. The results showed that (i) the nitrite‐N degradation rate of the 8DO17 strain was 99%; (ii) a wide spectrum of pH (7.0–9.0) and concentration of nitrite‐N (0.5–10 mg L−1) and the highest rate of nitrite‐N degradation near to 100% under optimum conditions (35°C, salinity 30, pH 7.5) was explored; (iii) no significant differences were found in the survival, total haemocyte count, antibacterial activities and bacteriolytic activity of the shrimp between the treatments and the control (P > 0.05); (iv) for the use of real‐time PCR based on 16S rDNA test, detection limit is 103 DNA copies and an excellent correlation coefficients (R² = 0.999) was obtained; and (v) in the application of real‐time PCR assay in four nitrite‐N degrading samples, highly significant positive correlation relation (P < 0.01) was found between log copy number of 16S rDNA and the rate of nitrite‐N degradation. The results suggest the real‐time PCR is a very rapid and sensitive technique for monitoring the dynamic changes and assessing the effect in practical application of the 8DO17 strain in aquatic water.

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“…The denitrifying bacteria are mostly heterotrophic facultative anaerobes; therefore, the presence of an organic carbon source is essential for implementation of this usually anaerobic or anoxic process. As an inexpensive and easily available source of carbon, methanol has been used and optimized for biological nitrate removal (Pungrasmi et al 2013, Timmermans & van Haute 1983). The immobilization of bacterial cells has been studied as a way to improve the performance of the denitrification process.…”

Section: Introductionmentioning

confidence: 99%

Use of Ion Exchange Beads as a Bacterial Carrier for Biodenitrification

Foglar

Šiljeg

Babić³

2016

Journal AWWA

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In this research, an exhausted ion exchange resin was used as a carrier of bacterial cells, and the resulting bio‐ion exchange resin beads were applied in batch and continuous‐flow experiments for monitoring nitrate removal. The continuous‐flow experiments investigated the effects of hydraulic retention times (HRTs) of 7–24 h on the course of denitrification; nitrate removal was achieved at HRTs longer than 9 h, with nitrate and organic removal efficiencies higher than 99 and 80%, respectively. Nitrate removal was then monitored continuously for 31 days. To investigate the influence of phosphate salts on denitrification performance, raw Cetina River water was used. The research also examined the effect of methanol addition on denitrification by decreasing the methanol‐to‐nitrogen mass ratio to 2.5:1. The obtained nitrate and organic removal efficiencies of 100 and 93%, respectively, suggest that the application of the resin beads was effective for continuous nitrate removal from surface water.

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Optimization and evaluation of a bottom substrate denitrification tank for nitrate removal from a recirculating aquaculture system

Cited by 24 publications

References 21 publications

Effects of Salinity and Immobilization Period on the Nitrification and Denitrification Co-processes during Biofilter Acclimation in a Marine Recirculating Aquaculture System

Effects of Salinity and Immobilization Period on the Nitrification and Denitrification Co-processes during Biofilter Acclimation in a Marine Recirculating Aquaculture System

Identification and real-time qPCR quantification of a nitrite-N degrading bacterial strain in aquatic water

Use of Ion Exchange Beads as a Bacterial Carrier for Biodenitrification

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