Quantification of Nucleated Cells, CD34-Positive Cells and CFU-GM Colonies in Single Bone Marrow Samples and Bone Marrow Harvests Derived from Healthy Children

Abstract

Little is known regarding bone marrow (BM) cellularity, CD34+ fraction, and CFU-GM colony formation in relation to age and whether healthy children require a reference range distinct from healthy adults. We therefore analyzed a series of single BM aspirates from 45 healthy children who were evaluated as potential BM donors. Thirty-three of these children subsequently donated BM. We quantified the nucleated cell count, fraction of CD34+ cells, and number of CFU-GM colonies in single aspirates and BM harvests. Single aspirates displayed a mean nucleated cell count of 31.3 × 10(6) cells/mL, a mean fraction of 1.17% CD34+ cells, and a mean colony forming potential of 66.6 CFU-GM/10(5) cells. Harvests yielded the same number of nucleated cells but increased numbers of CD34+ cells and CFU-GM compared with single aspirates. The mean nucleated cell count in BM harvests was 31.1 × 10(6) /mL with a mean fraction of 1.95% CD34+ cells and a mean of 112.4 CFU-GM colonies/10(5) cells. The concentration of nucleated cells was elevated compared with reported adult counts, while CD34+ percentage and CFU-GM counts were similar. In this series of healthy children, the fraction of CD34+ cells, CFU-GM colonies, and nucleated cells decreased with age. We did not identify gender specific differences. To our knowledge, this represents the first comprehensive study of CD34+ cell fraction, CFU-GM counts, and nucleated cell numbers in the BM of healthy children. The findings provide valuable information for practical use for BM transplantation and contribute to the understanding of hematopoiesis from birth to adulthood.

Hematopoietic stem cell and mesenchymal stem cell population size in bone marrow samples depends on patient’s age and harvesting technique

“…Many studies show that regenerative potential of HSC declines. Beside studies describing decrease in HSCs number with age (Kuranda et al 2011;Schündeln et al 2014;Kresnik et al 2016), some other studies reported an increase in HSC number or in the number of HSC subpopulations with age (Kuranda et al 2011;Pang et al 2011). In our count, the concentration of hematopoietic CD45 dim /CD34 ?…”

Bone Marrow-Derived Progenitor Cells Are Functionally Impaired in Ischemic Heart Disease

“…For example, evidence has been collected for age-dependent deterioration of the proliferative capacity of BM-derived progenitor cells and in general the hematopoietic compartment [ 24 ]. In healthy subjects and/or CAD patients, age has been associated with impaired in vivo neovascularization, in vitro migration, and myeloid differentiation capacity of BM-MNC [ 14 , 40 ]. This age effect was also observed in our study population: a negative relation was found with both GM-CFU and BFU-E numbers; however, this bias was limited since our study cohorts did not differ with respect to age.…”

Bone marrow derived CD 34+ cells and leukocytes in 729 children and adults with non-malignant diseases

“…Furthermore, our ndings (higher cell counts in younger patients) support a previous study with a smaller sample size. 6 Autologous BM-derived cell-based therapies in regenerative medicine are on the rise. While it is generally known that BMA contains a mix of nucleated cells and other biologics such as growth factors and exosomes 13 , comprehensive information regarding normal ranges of leucocytes and/or CD 34 + cell counts in BMA and BMAC in a large series of patients of all age groups was missing.…”