Structural characterization of polysaccharides expressed by Burkholderia oklahomensis E0147

Synthesis of the Tetrasaccharide Repeating Unit of the β-Kdo-Containing Exopolysaccharide from Burkholderia pseudomallei and B. cepacia Complex

“…The residue was purified by silica gel flash chromatography (PE/EtOAc 1:0 to 8:2) to give 3 (951 mg, 86%) as a white amorphous solid along with its 2-OTBS regioisomer (136 mg, 12%) as a colorless oil. Analytical data for 3: (10). 1,5-Cyclooctadiene-bis(methyldiphenylphosphine)iridium(I) hexafluorophosphate (58 mg, 68 μmol, 0.03 equiv) was dissolved in anhydrous THF (11 mL), and the resulting red solution was degassed under Ar.…”

“…This EPS is thus seen as a promising target for the development of diagnostics and/or subunit vaccines against melioidosis. 8,9 Interestingly, this β-Kdo-containing EPS has also been isolated from the human pathogenic B. oklahomensis E0147 strain 10 as well as from four different species of the B. cepacia complex (Bcc), that is, B. ambifaria, B. cepacia, B. dolosa, and B. stabilis. 11,12 Cystic fibrosis patients are strongly susceptible to Bcc infections, known as the Cepacia syndrome, leading to rapid death in 20% of infected patients.…”

“…Furthermore, a monoclonal IgG (mAb 3015) with specificity for this EPS was isolated and exhibited no cross-reactivity with other Burkholderia species with the exception of the closely genetically related B. mallei , the causative agent of glanders. This EPS is thus seen as a promising target for the development of diagnostics and/or subunit vaccines against melioidosis. , Interestingly, this β-Kdo-containing EPS has also been isolated from the human pathogenic B. oklahomensis E0147 strain as well as from four different species of the B. cepacia complex (Bcc), that is, B. ambifaria , B. cepacia , B. dolosa , and B. stabilis . , Cystic fibrosis patients are strongly susceptible to Bcc infections, known as the Cepacia syndrome, leading to rapid death in 20% of infected patients. Noteworthily, vaccination is currently unavailable to prevent Bcc infections …”

Effect of temperature on Burkholderia pseudomallei growth, proteomic changes, motility and resistance to stress environments

“…B . pseudomallei encodes numerous virulence factors including cell-associated and secretory factors such as type 3 and type 6 secretion systems, proteinases, lipase, lipopolysaccharide, capsule, biofilm and flagella 8 . Many of these are regulated by bacterial and environmental factors including quorum-sensing systems 9 , 10 , VirAG two-component regulatory system 11 , acid 12 , salt stress 13 and nutritional availability 14 .…”