Biodegradable magnetic calcium phosphate nanoformulation for cancer therapy

Tang, Zhaomin; Zhou, Yangbo; Sun, Huili; Li, Dan; Zhou, Shaobing

doi:10.1016/j.ejpb.2014.01.003

Cited by 21 publications

(12 citation statements)

References 34 publications

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“…Our idea was to produce non-toxic magnetic particles with a surface able to bind DNA and to deliver it inside cells. 35 The presence of the polycation though did not eliminate the potential toxicity of the formulation. An outer biodegradable calcium phosphate layer was highly preferred to iron oxide surface modication with generally more efficient but cytotoxic polycations.…”

Section: Resultsmentioning

confidence: 98%

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Magnetically deliverable calcium phosphate nanoparticles for localized gene expression

et al. 2015

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Iron oxide doped tricalcium phosphate (Fe 2 O 3 @TCP) nanoparticles were designed as transfection vehicles and prepared by flame spray synthesis. Both components are known to be non-toxic and biocompatible.Calcium phosphate (CaP) facilitates DNA entry into cells without the need for toxic cationic mediators, while magnetic iron oxide allows for particle localization at a target site. Flame spray synthesis ensures easy and low-cost nanoparticle production in a reproducible way. Fe 2 O 3 @TCP nanoparticles, exhibiting DNA-binding capacity in the presence of CaCl 2 , were tested for transfection of a green fluorescent protein (GFP) encoding plasmid with Human Embryonic Kidney 293 (HEK 293) cells. Commercial magnetic agents, polyethylenimine (PEI) and standard calcium phosphate-mediated transfection were used for comparison. Transfection efficiency was estimated by GFP expression detected by fluorescence microscopy, while hoechst/ethidium homodimer-1 staining allowed the evaluation of method toxicity.We were able to efficiently transfect HEK 293 cells, and showed that Fe 2 O 3 @TCP particles and bound DNA can be concentrated in specific sites in a culture plate through the application of a magnetic field gradient to achieve localized transfection. While the commercial magnetic controls strongly affected cell growth and morphology, Fe 2 O 3 @TCP particles did not show marked toxicity and had only limited effects on cell proliferation. Overall performance in terms of transfection efficiency, cell proliferation and viability, were comparable to that of CaP and PEI, which lack magnetic targeting capability. The newly synthetized Fe 2 O 3 @TCP are, therefore, improved tools to deliver nucleic acids into cells and achieve spatial control of transfection. † Electronic supplementary information (ESI) available: Particle characterization methods; TEM micrograph of Fe 2 O 3 @TCP nanoparticles; particle density calculation; primary particle size and number of particle per aggregate calculations; procedure for preparation and transformation of chemically competent E. coli; DNA binding assay; microscopy; live/dead assay and cell counting procedure; statistical analysis. See

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Section: Resultsmentioning

confidence: 98%

“…34 An earlier attempt to produce magnetic CaP nanoparticles in a multi-step synthesis, comprising biomineralization of CaP on PEI-coated magnetic beads, has also been made. 35 The presence of the polycation though did not eliminate the potential toxicity of the formulation.…”

Section: Resultsmentioning

confidence: 98%

Magnetically deliverable calcium phosphate nanoparticles for localized gene expression

et al. 2015

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“…In addition, these nanoparticles can be functionalised with different functionalised groups, e.g., carboxyl, amines, biotin, polyethyleneimine and antibodies, allowing several methods of DNA or RNA attachment and, furthermore, suitable delivery of small interfering RNA or oligonucleotides under in vitro and in vivo conditions. One study carried out by Tang et al (2014) describes the preparation of magnetic calcium phosphate nano-formulations that were used for transfection of DNA [78]. In the same way, any other divalent metal cations, such as Mg +2 , Mn +2 and Ba +2 can form ionic complexes with the DNA (similar to Ca +2 ), providing stabilization to DNA structures.…”

Section: Non-viral Vectors For Gene Deliverymentioning

confidence: 99%

Successes and Challenges: Inhaled Treatment Approaches Using Magnetic Nanoparticles in Cystic Fibrosis

2020

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Magnetic nanoparticles have been largely applied to increase the efficacy of antibiotics due to passive accumulation provided by enhancing permeability and retention, which is essential for the treatment of lung infections. Recurring lung infections such as in the life-shortening genetic disease cystic fibrosis (CF) are a major problem. The recent advent of the CF modulator drug ivacaftor, alone or in combination with lumacaftor or tezacaftor, has enabled systemic treatment of the majority of patients. Magnetic nanoparticles (MNPs) show unique properties such as biocompatibility and biodegradability as well as magnetic and heat-medicated characteristics. These properties make them suitable to be used as drug carriers and hyperthermia-based agents. Hyperthermia is a promising approach for the thermal activation therapy of several diseases, including pulmonary diseases. The benefits of delivering CF drugs via inhalation using MNPs as drug carriers afford application of sufficient therapeutic dosages directly to the primary target site, while avoiding potential suboptimal pharmacokinetics/pharmacodynamics and minimizing the risks of systemic toxicity. This review explores the multidisciplinary approach of using MNPs as vehicles of drug delivery. Additionally, we highlight advantages such as increased drug concentration at disease site, minimized drug loss and the possibility of specific cell targeting, while addressing major challenges for this emerging field.

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“…Interaction of CPP with the endosomal membrane as well as the positive zeta potential of CPP-lipid nanoparticles could facilitate the transfer of siRNA into the cytoplasm. 65 …”

Section: Conjugation Of Lipid Nanoparticles With Cell-penetrating Pepmentioning

confidence: 99%

Cell-penetrating peptides and their analogues as novel nanocarriers for drug delivery

Jafari¹,

Dizaj²,

Adibkia³

2017

Bioimpacts

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Introduction: The impermeability of biological membranes is a major obstacle in drug delivery; however, some peptides have transition capabilities of biomembranes. In recent decades, cell-penetrating peptides (CPPs) have been introduced as novel biocarriers that are able to translocate into the cells. CPPs are biologically potent tools for non-invasive cellular internalization of cargo molecules. Nevertheless, the non-specificity of these peptides presents a restriction for targeting drug delivery; therefore, a peptidic nanocarrier sensitive to matrix metalloproteinase (MMP) has been prepared, called activatable cell-penetrating peptide (ACPP). In addition to the cell-penetrating peptide dendrimer (DCPP), other analogues of CPPs have been synthesized. Methods: In this study, the most recent literature in the field of biomedical application of CPPs and their analogues, ACPP and DCCP, were reviewed. Results: This review focuses on CPP and its analogues, ACPP and DCPP, as novel nanocarriers for drug delivery. In addition, nanoconjugates and bioconjugates of these peptide sequences are discussed. Conclusion: DCCP, branched CPPs, compared to linear peptides have advantages such as resistance to rapid biodegradation, high loading capacities and large-scale production capability.

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Biodegradable magnetic calcium phosphate nanoformulation for cancer therapy

Cited by 21 publications

References 34 publications

Magnetically deliverable calcium phosphate nanoparticles for localized gene expression

Magnetically deliverable calcium phosphate nanoparticles for localized gene expression

Successes and Challenges: Inhaled Treatment Approaches Using Magnetic Nanoparticles in Cystic Fibrosis

Cell-penetrating peptides and their analogues as novel nanocarriers for drug delivery

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